Small animal imaging of cardiovascular disease using single photon emission tomography (SPECT) can be used to provide quantitative measurements of myocardial infarct. The purpose of this study was to demonstrate the accuracy of pinhole SPECT imaging with [99mTc]sestamibi for estimation of infarct size in a rat model of coronary artery disease. Nine rats had their left anterior descending artery ligated to induce a region of myocardial infarct. These animals were injected with 37 MBq [99mTc]sestamibi, and, 1 h later, scanned on a pinhole SPECT system for 30 min. The defect size measured with SPECT, which was dependent on a threshold applied to the short axis circumferential profiles, was compared against the gold standard triphenyltetrazolium chloride (TTC) staining. The size of the perfusion deficit measured using [99mTc]sestamibi SPECT compared very favorably with the TTC staining result, for threshold values in the range 50–70%. The optimum threshold was approximately 70%, giving an excellent correlation (R2=0.89, p<0.001). Estimation of infarct size by [99mTc] sestamibi SPECT yielded an excellent agreement with TTC staining. In conclusion, measurement of myocardial infarct with SPECT can be used to study the rat heart in vivo, and provides a quantitative measure of myocardial viability.
ischemia myocardium pinhole SPECT rat sestamibi triphenyltetrazolium chloride staining
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This research was supported by National Institutes of Health grants EB-002473 (RZ), and EB-001809 (PDA). DT is supported by American Heart Association grant 0425558U.
Acton PD, Choi SR, Plössl K and Kung HF (2002) Quantification of dopamine transporter in mouse brain using ultra-high resolution single photon emission tomography. Eur J Nucl Med 29:691–698CrossRefGoogle Scholar
Acton PD, Hou C, Kung MP, Plössl K, Keeney CL and Kung HF (2002) Occupancy of dopamine D2 receptors in the mouse brain measured using ultra-high resolution single photon emission tomography and [123I]IBF. Eur J Nucl Med 29:1507–1515CrossRefGoogle Scholar
Auricchio A, Acton PD, Hildinger M, Plössl K, Kung HF and Wilson JM (2003) In vivo quantitative non-invasive imaging of gene transfer with single-photon emission computerized tomography. Hum Gene Ther 14:255–261CrossRefPubMedGoogle Scholar
Kizana E and Alexander IE (2003) Cardiac gene therapy: therapeutic potential and current progress. Current Gene Therapy 3:418–451CrossRefGoogle Scholar
Timmermans F, De Sutter J and Gillebert TC (2003) Stem cells for the heart, are we there yet? Cardiology 100:176–185CrossRefPubMedGoogle Scholar
Ostrowski RP, Januszewski S, Kowalska Z and Kapuscinski A (2003) Effect of endothelin receptor antagonist bosentan on plasma leptin concentration in acute myocardial infarction in rats. Pathophysiology 9:249–256CrossRefPubMedGoogle Scholar
O’Conner MK, Hammell T and Gibbons RJ (1990). In vitro validation of a simple tomographic technique for estimation of percentage myocardium at risk using methoxyisobutyl isonitrile technetium 99 m (sestamibi). Eur J Nucl Med 17:69–76CrossRefPubMedGoogle Scholar
Wu MC, Gao DW, Sievers RE, Lee RJ, Hasegawa BH and Dae MW (2003). Pinhole single photon emission computed tomography for myocardial perfusion imaging of mice. J Am Coll Cardiol 42:576–582CrossRefPubMedGoogle Scholar
Liu Z, Kastis GA, Stevenson GD, Barrett HH, Furenlid LR, Kupinski MA et al (2002). Quantitative analysis of acute myocardial infarct in rat hearts with ischemia-reperfusion using a high resolution stationary SPECT system. J Nucl Med 43:933–939PubMedGoogle Scholar