Breast Cancer Research and Treatment

, Volume 101, Issue 2, pp 161–174 | Cite as

Lewis x Antigen Mediates Adhesion of Human Breast Carcinoma Cells to Activated Endothelium. Possible Involvement of the Endothelial Scavenger Receptor C-type Lectin

  • María Teresa Elola
  • Mariana Isabel Capurro
  • María Marcela Barrio
  • Peter J. Coombs
  • Maureen E. Taylor
  • Kurt Drickamer
  • José Mordoh
Preclinical Study


Lewis x (Lex, CD15), also known as SSEA-1 (stage specific embryonic antigen-1), is a trisaccharide with the structure Galβ(1–4)Fucα(1–3)GlcNAc, which is expressed on glycoconjugates in human polymorphonuclear granulocytes and various tumors such as colon and breast carcinoma. We have investigated the role of Lex in the adhesion of MCF-7 human breast cancer cells and PMN to human umbilical endothelial cells (HUVEC) and the effects of two different anti-Lex mAbs (FC-2.15 and MCS-1) on this adhesion. We also analyzed the cytolysis of Lex+-cells induced by anti-Lex mAbs and complement when cells were adhered to the endothelium, and the effect of these antibodies on HUVEC. The results indicate that MCF-7 cells can bind to HUVEC, and that MCS-1 but not FC-2.15 mAb inhibit this interaction. Both mAbs can efficiently lyse MCF-7 cells bound to HUVEC in the presence of complement without damaging endothelial cells. We also found a Lex-dependent PMN interaction with HUVEC. Although both anti-Lex mAbs lysed PMN in suspension and adhered to HUVEC, PMN aggregation was only induced by mAb FC-2.15. Blotting studies revealed that the endothelial scavenger receptor C-type lectin (SRCL), which binds Lex-trisaccharide, interacts with specific glycoproteins of M r␣∼␣28 kD and 10 kD from MCF-7 cells. The interaction between Lex+-cancer cells and vascular endothelium is a potential target for cancer treatment.


Lewis x (CD15), Monoclonal antibodies FC 2.15 and MCS 1, MCF 7, PMN, Scavenger receptor C type lectin 







Cluster of Differentiation


complement-dependent cytotoxicity


carcinoembryonic antigen


carcinoembryonic antigen-related cell adhesion molecule 1


dendritic cell


dendritic cell-specific [intercellular adhesion molecule]-3- grabbing nonintegrin




Fluorescence-activated cell sorting


fluorescein isothiocyanate




human umbillical vein endothelial cells


iodoacetic acid


intercellular adhesion molecule-3




Lewis x

Galβ1–4(Fucα1–3) GlcNAc

Lewis x


Lewis a

Galβ1–3(Fucα1–4) GlcNAc

Lewis a


Silayl-Lewis x

NeuAcα2–3 Galβ1– 4(Fucα1–3) GlcNAc

Silayl-Lewis x


monoclonal antibody


phosphate buffered saline


polymorphonuclear neutrophils




scavenger receptor C-type lectin


stage specific embryonic antigen 1


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This work has been supported by grants from Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT), Fundación Sales, Fundación para la Investigación y Prevención del Cancer (FUCA), Fundación Pedro F. Mosoteguy, Fundación María Calderón de la Barca, Argentina, the Wellcome Trust and the Biotechnology and Biological Sciences Research Council. JM and MTE are members of CONICET. We are grateful to the Obstetric Department, Hospital Rivadavia, Argentina, for providing umbilical cords from normal volunteers. We also acknowledge the Hematology and Obstetric Departments, Hospital Naval Pedro Mallo, Argentina, for the provision of blood/human serum, and umbilical cords, respectively, from normal volunteers.


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Copyright information

© Springer Science+Business Media B.V. 2006

Authors and Affiliations

  • María Teresa Elola
    • 1
  • Mariana Isabel Capurro
    • 2
  • María Marcela Barrio
    • 3
  • Peter J. Coombs
    • 4
  • Maureen E. Taylor
    • 4
  • Kurt Drickamer
    • 4
  • José Mordoh
    • 1
    • 3
  1. 1.Fundación Instituto LeloirBuenos AiresArgentina
  2. 2.Division of Molecular and Cell Biology, Sunnybrook and Women’s College Health Sciences Center and Department of Medical BiophysicsUniversity of TorontoOntarioCanada
  3. 3.Centro de Investigaciones Oncológicas – FUCABuenos AiresArgentina
  4. 4.Division of Molecular Biosciences, Biochemistry BuildingImperial CollegeLondon United Kingdom

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