The sodium-dependent di- and tricarboxylate transporter, NaCT, is not responsible for the uptake of D-, L-2-hydroxyglutarate and 3-hydroxyglutarate into neurons
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Concentrations of glutarate (GA) and its derivatives such as 3-hydroxyglutarate (3OHGA), D- (D-2OHGA) and L-2-hydroxyglutarate (L-2OHGA) are increased in plasma, cerebrospinal fluid (CSF) and urine of patients suffering from different forms of organic acidurias. It has been proposed that these derivatives cause neuronal damage in these patients, leading to dystonic and dyskinetic movement disorders. We have recently shown that these compounds are eliminated by the kidneys via the human organic anion transporters, OAT1 and OAT4, and the sodium-dependent dicarboxylate transporter 3, NaDC3. In neurons, where most of the damage occurs, a sodium-dependent citrate transporter, NaCT, has been identified. Therefore, we investigated the impact of GA derivatives on hNaCT by two-electrode voltage clamp and tracer uptake studies. None of these compounds induced substrate-associated currents in hNaCT-expressing Xenopus laevis oocytes nor did GA derivatives inhibit the uptake of citrate, the prototypical substrate of hNaCT. In contrast, D- and L-2OHGA, but not 3OHGA, showed affinities to NaDC3, indicating that D- and L-2OHGA impair the uptake of dicarboxylates into astrocytes thereby possibly interfering with their feeding of tricarboxylic acid cycle intermediates to neurons.
KeywordsPyruvate Carboxylase Glutaric Acid Xenopus Laevis Oocyte Excitatory Amino Acid Transporter Glutaric Aciduria Type
Glutaric aciduria type I is caused by deficiency of the enzyme glutaryl CoA-dehydrogenase (GCDH) in the metabolic pathway of lysine, hydroxylysine, and tryptophan, leading to an accumulation of glutaric acid (GA) and its derivative 3-hydroxy glutaric acid (3OHGA) (Funk et al. 2005; Harting et al. 2009; Hedlund et al. 2006; Strauss et al. 2010) in blood, cerebrospinal fluid, and urine. 2-Hydoxyglutaric (2OHGA) acidurias are characterized by the presence of elevated concentrations of either L-2OHGA or its enantiomer D-2OHGA and of α-ketoglutarate (αKG) in body fluids (Read et al. 2005; Seiijo-Martinez et al. 2005; Struys 2006; Struys et al. 2007). Whereas L-2OHGA aciduria is caused by mutations of the FAD-dependent L-2OHGA dehydrogenase (Rzem et al. 2004), the underlying metabolic defects of D-2OHGA aciduria are due to mutations of the enzyme D-2-hydroxyglutarate dehydrogenase (Struys et al. 2005). It is assumed that all GA derivatives are neurotoxins and cause neuronal damage.
We have recently identified the sodium-dependent dicarboxylate transporter 3 (NaDC3) and the organic anion transporter 1 (OAT1) to be responsible for the uptake of GA and its derivatives from the blood into renal proximal tubular cells (Hagos et al. 2008; Mühlhausen et al. 2008). Both transporters are also present in the human brain (Alebouyeh et al. 2003; Bleasby et al. 2006). Whereas OAT1 is involved in the efflux of various neurotransmitter metabolites from the cerebrospinal fluid to the blood across the choroid plexus (Alebouyeh et al. 2003), NaDC3 is restricted to astrocytes (Yodoya et al. 2006) and possibly the choroid plexus (Pajor et al. 2001). Besides NaDC3, another electrogenic sodium-dependent di- or tricarboxylate transporter, hNaCT, has been identified in the brain. NaCT is preferentially located in neurons, especially in the hippocampus, cerebellum, cerebral cortex and olfactory bulb (Inoue et al. 2002a, b; Wada et al. 2006; Yodoya et al. 2006). Since neuropathological findings observed in patients suffering from glutaric aciduria type 1 as well as from D- and L-2OHGA aciduria occur in neurons, we investigated the impact of GA derivatives on hNaCT expressed in Xenopus laevis oocytes.
Materials and methods
In vitro transcription of hNaCT- and hNaDC3-cRNA
Capped cRNA from the human NaCT (GenBank Accession No. AY151833) and human NaDC3 (GenBank Accession No. AF154121) was used as a template for cRNA synthesis. Plasmids were linearized with Not I and in vitro cRNA transcription was performed using the T7 mMessage mMaschine kit (Ambion, Austin, TX, USA) according to the manufactor’s instructions. The resulting cRNA was suspended in purified, RNAse-free water to a final concentration of 1 μg/μl.
A standard oocyte Ringer solution (ORi) was used for oocyte preparation, storage, and for the uptake as well as for the electrophysiologic measurements. ORi contained (in mM): 110 NaCl, 3 KCl, 2 CaCl2, 5 HEPES/Tris, adjusted to pH 7.5. Citrate, succinate, glutarate (GA), its derivatives, 3-hydroxyglutarate (3OHGA), D- (D-2OHGA), and L-2-hydroxyglutarate (L-2OHGA), and glutamate were added to ORi in the concentrations indicated in the figure legends and pH was adjusted to 7.5. All chemicals, including those for ORi, for oocyte preparation and storage, as well as for the uptake and electrophysiologic experiments were purchased from Sigma-Aldrich (Taufkirchen, Germany). 3OHGA was obtained from C. Mühlhausen (UKE, Hamburg, Germany).
Oocyte preparation and storage
Stage V and VI oocytes from Xenopus laevis (Nasco, Fort Atkinson, WI, USA) were separated by an overnight treatment with collagenase (Typ CLS II; Biochrom, Berlin, Germany), subsequent washings in calcium-free ORi and maintained at 16–18°C in ORi containing a calcium concentration of 2 mM. One day after removal from the frog, oocytes were injected with 23 nl cRNA coding either for hNaCT or hNaDC3, or an equivalent amount of water (mocks) and maintained at 16–18°C in ORi supplemented with 50 μM gentamycin and 2.5 mM sodium pyruvate. After 3–4 days of incubation with daily medium changes, oocytes were used for tracer uptake studies.
Uptake of [14C]citrate (14C[1,5]citric acid; GE Health Care, Freiburg, Germany) or [14C]glutarate (14C[1,5]glutaric acid; MP Biomedicals, Heidelberg, Germany) in hNaCT- or of [14C]succinate (14C[2,3]succinic acid; Perkin Elmer, Rodgau, Germany) in hNaDC3-expressing oocytes was assayed at room temperature. Inhibition of citrate uptake was determined by simultaneous application of 12 μM 14C-citrate and ORi containing 1 mM of GA, 3OHGA, D- or L-2OHGA, respectively, for 30 min. 14C-glutarate was used at a concentration of 20 μM. Inhibition of succinate uptake was determined by simultaneous application of 18 μM 14C-succinate plus 40 μM sodium succinate and ORi containing 1 mM glutamate. After incubation in the respective solutions, radioactivity was aspirated and the oocytes were washed twice in ice-cold ORi. Oocytes were dissolved by gently shaking for 2 h in 100 μl 1 N NaOH, neutralized with 100 μl 1 N HCl, and their 14C-contents were determined by liquid scintillation counting (Tricarb 2900TR; Perkin Elmer). Mocks were treated in a similar manner to serve as controls. Inhibition experiments were performed at least in duplicate with 8–10 oocytes for each experimental condition.
These studies were carried out 3–4 days after cRNA injection at room temperature. Oocytes were placed into a 0.5-ml chamber on the stage of a microscope and impaled under direct view with borosilicate glass microelectrodes filled with 3 M KCl (BioMedical Instruments, Zöllnitz, Germany). Current-voltage (I-V) recordings were performed using a two-electrode voltage clamp device (OC725A; Warner, Hambden, CT, USA) in the voltage clamp mode. Substrate-associated currents were obtained by subtraction of the currents in the presence from those in the absence of the substrate of interest.
Statistics and calculations
Data are provided as means ± SEM. Paired Student’s t test was used to show statistically significant difference of citrate uptake in the absence and presence of the GA derivatives or of succinate uptake in the absence and presence of glutamate. Statistical significance was set at p < 0.01. Michaelis-Menten constants (KM) for 3OHGA, D- and L-2OHGA were determined by SigmaPlot software (Systat Software, Point Richmond, CA, USA) using the Michaelis-Menten equation I = Imax · [S]/(KM + [S]), where I is the current, Imax is the maximum current observed at saturating substrate concentrations, KM is the substrate concentration at half-maximal current, and S is the substrate concentration.
To date, the brain is the only mammalian tissue from which NaCT has been cloned (Inoue et al. 2002a, b). Subsequent studies have shown that its expression is restricted to neurons (Wada et al. 2006; Yodoya et al. 2006). Therefore, we reasoned that hNaCT might be able to accept GA derivatives which were suggested to induce the neuronal damage observed in patients suffering from glutaric aciduria type 1 and D, L-hydroxyglutaric aciduria. However, these compounds neither induced substrate-associated currents nor did they inhibit the uptake of citrate, the prototypical substrate of hNaCT, in hNaCT-expressing oocytes. In addition, we found no interaction of hNaCT with αKG, a compound recently described to be taken up by neurons (Shank and Bennett 1993). Consequently, uptake of the GA derivatives into neurons by NaCT is not the cause of the observed neurodegeneration.
Particularly for glutaric aciduria type 1, it is suggested that glutaryl-CoA, GA, and 3OHGA accumulating in the synaptic cleft is involved in the pathogenesis of the disease (Kölker et al. 2004; Strauss et al. 2010). Because these compounds do not readily cross the blood-brain barrier, they accumulate within the brain (Kölker et al. 2002a, b; Junqueira et al. 2003, 2004; Porciuncula et al. 2004; Wajner et al. 2004; Gerstner et al. 2005; Sauer et al. 2006), where they may inhibit the delivery of neurometabolic precursors.
Due to the absence of pyruvate carboxylase (Cesar and Hamprecht 1995; Hassel 2001), neurons lack the capacity to perform de novo synthesis of tricarboxylic cycle constitutents (TCA) and therefore depend on extracellular sources of TCA intermediates to replenish intracellular pools of neurotransmitters, such as glutamate and γ-aminoisobutyrate (GABA). Astrocytes, which in contrast to neurons, express pyruvate carboxylase (Shank et al. 1985), play a major role in feeding TCA constituents to the neurons. Since NaCT is localized in neurons, we assumed that NaCT is responsible not only for the uptake of citrate but also for the uptake of other tri- and dicarboxylic acid derivatives into neurons. This, however, was not the case: GA and αKG were not accepted by NaCT.
The synaptic action of glutamate is terminated by its uptake into astrocytes and or into presynaptic neurons. Uptake of glutamate into astrocytes occurs by distinct excitatory amino acid transporters (EAATs) and possibly by low affinity sodium-dependent transport systems (Danboldt 2001; Holten et al. 2008). One such candidate transporter may be NaDC3, recently localized to astrocytes (Yodoya et al. 2006). However, glutamate neither induced inward currents nor inhibited succinate uptake in hNaDC3-expressing oocytes, and 1 mM glutamate reduced succinate uptake only by 19.9 ± 8.5 % (N.S.). Therefore, hNaDC3 can be excluded as a transporter facilitating glutamate uptake into astrocytes as opposed to recent speculation (Frizzo et al. 2004; Holten et al. 2008).
At –60 mV, hNaDC3 carries αKG and GA with high and the other GA derivatives with moderate affinity (Hagos et al. 2008). In the present study, we found that the affinities for D- and L-2OHGA increased whereas that of 3OHGA decreased at depolarized potentials. Besides KM, Imax was also influenced by the membrane potential. The different GA derivatives also differ in their Imax being largest for D-2OHGA, indicating that D-2OHGA can be taken up into astrocytes at high rates and may interfere with the uptake of αKG which is needed by astrocytes for the synthesis of glutamine (Peng et al. 1991) and other metabolic purposes.
Although our findings did not elucidate the mechanisms by which the GA derivatives induce the neuronal damage observed in the affected patients, we can definitely exclude NaCT as a transporter facilitating the uptake of D- and L-2OHGA as well as 3OHGA into neurons. The impaired uptake of αKG due to the efficient interactions of D- and L-2OHGA with NaDC3 could interfere with the astrocytes ability to produce TCA derivatives and to feed neurons with these compounds needed for synthesis of neurotransmitters. As, during brain development, energy requirements are increasing (Harting et al. 2009), the metabolic support of astrocytes may be limited due to accumulating GA derivatives in the synaptic cleft. This in turn may induce the observed neuronal damage due to shortage of fuels when the brain is in a most vulnerable phase as recently discussed by Strauss et al. (2010).
The hNaCT clone was a generous gift of V. Ganapathy, PhD, Medical College of Georgia, Augusta, USA. We also thank C. Mühlhausen, MD, Dept. Pediatrics, University Medical Center Hamburg-Eppendorf, Hamburg, Germany for the generous gift of 3OHGA. The authors are grateful for financial support by the Else Kröner-Fresenius-Stiftung (P22/07 // A19/07). The technical assistance of I. Markmann is gratefully acknowledged.
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