Biomedical Microdevices

, 19:13 | Cite as

Investigation of osteogenic activity of primary rabbit periosteal cells stimulated by multi-axial tensile strain



Periosteum–derived cells was indicated to respond to mechanical force and have stem cell potential capable of differentiating into multiple tissue. Investigation of osteogenic activity under mechanical stimulation is important to understand the therapeutic conditions of fracture healing. In this work, a cell culture platform was developed for respectively providing isotropic and anisotropic axial strain. Primary rabbit periosteal cells were isolated and cultured in the chamber. Multi-axial tensile strain was received and osteogenic activity was investigated by mRNA expressions of CBFA1 and OPN. The highest mRNA expression was found in moderate strain (5-8%) under anisotropic axial strain. These results provided important foundation for further in vivo studies and development of tailor-made stretching rehabilitation equipment.


Periosteal cells Osteogenic activity Orthopedics Rehabilitation 



This work was supported by Chang Gung Memorial Hospital, Linkou branch, Taiwan (Project no. BMRPC05).


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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  1. 1.Bone and Joint Research Center, Department of Orthopedic SurgeryChang Gung Memorial Hospital, Tooyuan BranchTaoyuanTaiwan
  2. 2.Graduate Institute of Medical MechatronicsChang Gung UniversityTaoyuanTaiwan
  3. 3.Department of Mechanical EngineeringChang Gung UniversityTaoyuanTaiwan
  4. 4.Department of Radiation OncologyChang Gung Memorial Hospital, Linkou BranchTaoyuanTaiwan

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