Investigation of osteogenic activity of primary rabbit periosteal cells stimulated by multi-axial tensile strain
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Periosteum–derived cells was indicated to respond to mechanical force and have stem cell potential capable of differentiating into multiple tissue. Investigation of osteogenic activity under mechanical stimulation is important to understand the therapeutic conditions of fracture healing. In this work, a cell culture platform was developed for respectively providing isotropic and anisotropic axial strain. Primary rabbit periosteal cells were isolated and cultured in the chamber. Multi-axial tensile strain was received and osteogenic activity was investigated by mRNA expressions of CBFA1 and OPN. The highest mRNA expression was found in moderate strain (5-8%) under anisotropic axial strain. These results provided important foundation for further in vivo studies and development of tailor-made stretching rehabilitation equipment.
KeywordsPeriosteal cells Osteogenic activity Orthopedics Rehabilitation
This work was supported by Chang Gung Memorial Hospital, Linkou branch, Taiwan (Project no. BMRPC05).
- C. De Bari, F. Dell'Accio, J. Vanlauwe, J. Eyckmans, I.M. Khan, C.W. Archer, E.A. Jones, D. McGonagle, T.A. Mitsiadis, C. Pitzalis, F.P. Luyten, Mesenchymal multipotency of adult human periosteal cells demonstrated by single-cell lineage analysis. Arthritis Rheum. 54, 1209–1221 (2006)CrossRefGoogle Scholar
- M. Ishijima, S.R. Rittling, T. Yamashita, K. Tsuji, H. Kurosawa, A. Nifuji, D.T. Denhardt, M. Noda, Enhancement of osteoclastic bone resorption and suppression of osteoblastic bone formation in response to reduced mechanical stress do not occur in the absence of osteopontin. J. Exp. Med. 193, 399–404 (2001)CrossRefGoogle Scholar
- K.F. Lei, Microfluidic systems for diagnostic applications: A review. JALA 17, 330–347 (2012)Google Scholar
- B. Mckibbin, The biology of fracture healing in long bones. J. Bone Joint Surg. 60B, 150–162 (1978)Google Scholar
- M. Samee, S. Kasugai, H. Kondo, K. Ohya, H. Shimokawa, S. Kuroda, Bone morphogenetic protein-2 (BMP-2) and vascular endothelial growth factor (VEGF) transfection to human periosteal cells enhances osteoblast differentiation and bone formation. J. Pharmacol. Sci. 108, 18–31 (2008)CrossRefGoogle Scholar
- Z. Sun, B.C. Tee, Molecular variations related to the regional differences in periosteal growth at the mandibular ramus. Bone Biol. 294, 79–87 (2011)Google Scholar
- N. Suzuki, Y. Yoshimura, Y. Deyama, K. Suzuki, Y. Kitagawa, Mechanical stress directly suppresses osteoclast differentiation in RAW264.7 cells. Int. J. Mol. Med 21, 291–296 (2008)Google Scholar
- E. Vogelin, N.F. Jones, J.I. Huang, J.H. Brekke, J.R. Lieberman, Healing of a critical-sized defect in the rat femur with use of a vascularized periosteal flap, a biodegradable matrix, and bone morphogenetic protein. J. Bone Joint Surg. Am. 87, 1323–1331 (2005)Google Scholar
- T. Yamate, H. Mocharla, Y. Taguchi, J.U. Igietseme, S.C. Manolagas, E. Abe, Osteopontin expression by osteoclast and osteoblast progenitors in the murine bone marrow: Demonstration of its requirement for osteoclastogenesis and its increase after ovariectomy. Endocrinology 138, 3047–3055 (1997)Google Scholar