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Biomedical Microdevices

, 19:12 | Cite as

A new phosphothreonine lyase electrochemical immunosensor for detecting Salmonella based on horseradish peroxidase/GNPs-thionine/chitosan

Article

Abstract

In the current study, a novel double-layer gold nanoparticles- electrochemical immunosensor electrode (DGN-EIE) immobilized with Salmonella plasmid virulence C (SpvC) antibody was developed. To increase the fixed quantity of antibodies and electrochemical signal, an electrochemical biosensing signal amplification system was utilized with gold nanoparticles-thionine-chitosan absorbing horseradish peroxidase (HRP). In addition, the SpvC monoclonal antibodies (derived from Balb/c mice) were prepared and screened with a high affinity to SpvC. To evaluate the quality of DGN-EIE, the amperometric I-t curve method was applied to determine Salmonella in PBS. The results showed that the response current had a good linear correlation with the bacterial quantity ranged from 1.0 × 101–5.0 × 104 cfu/mL. The lowest detection limit was found at 5 cfu/mL. Furthermore, the proposed immunosensor has been demonstrated with high sensitivity, good selectivity and reproducibility. Apparently, DGN-EIE may be a very useful tool for monitoring the bacteria.

Keywords

Phosphothreonine lyase Salmonella Electrochemical immunosensor Salmonella plasmid virulence C 

Notes

Acknowledgements

The work was supported by the National Natural Science Foundation of China (No. 31671857 and No. 31371773). In addition, we appreciate Shao-Feng lab for supplying SpvC recombinant plasmid.

Compliance with ethical standards

Conflict of interests

The authors declare that there is no conflict of interests regarding the publication of this paper.

Supplementary material

10544_2017_149_MOESM1_ESM.pdf (189 kb)
ESM 1 (PDF 189 kb)

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  1. 1.Biotechnology & Food Science CollegeTianjin University of CommerceTianjinChina
  2. 2.Tianjin Key Laboratory of Food BiotechnologyTianjinChina

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