Use of a CD laser pickup head to fabricate microelectrodes in polymethylmethacrylate substrates for biosensing applications

  • Jehú López-Aparicio
  • Mathieu Hautefeuille
  • Sara Herrera-Domínguez
  • Adriana Razo-de-León
  • Mariel Cano-Jorge
  • Ixchetl Rojas-Benito
  • Mariana Centeno-Sierra
  • Tatiana Fiordelisio-Coll
  • Catalina Elizabeth Stern-Forgach
Article

Abstract

In this work, we report a simple fabrication method for microelectrodes on a polymethylmethacrylate substrate, using a low-cost laser platform based on a CD-DVD unit for direct rapid-prototyping. We used this laser microfabrication technique to etch any desired design on polymethylmethacrylate substrates to produce microchannels with controlled geometry, with a highly repeatable micron-scale resolution. Those shallow microchannels were then filled with a conductive paste of material of our choice that was converted into microelectrodes of desired shapes and geometries after drying. To validate our process, different geometries, sizes and materials were used as electrodes, and then tested for amperometry and impedance measurements. Development of these microelectrodes is motivated by their potential application in sensors and biosensors, such as glucose and cell counting, as demonstrated in this paper.

Keywords

Microelectrodes Microfabrication Biosensing 

Notes

Acknowledgments

The authors thank LaNSBioDyT, DGAPA-PAPIIT grant #TA100315, CONACyT #271573 and #246988. JL thanks Aaró n Cruz-Ramírez and Diego Zamarrón-Hernández for their technical support.

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  • Jehú López-Aparicio
    • 1
    • 2
  • Mathieu Hautefeuille
    • 1
    • 2
  • Sara Herrera-Domínguez
    • 1
    • 2
  • Adriana Razo-de-León
    • 1
    • 2
  • Mariel Cano-Jorge
    • 1
    • 2
  • Ixchetl Rojas-Benito
    • 1
    • 2
  • Mariana Centeno-Sierra
    • 1
    • 2
  • Tatiana Fiordelisio-Coll
    • 1
    • 2
  • Catalina Elizabeth Stern-Forgach
    • 1
    • 2
  1. 1.Facultad de CienciasUniversidad Nacional Autónoma de MéxicoCiudad de MéxicoMéxico
  2. 2.Laboratorio Nacional de Soluciones Biomiméticas para Diagnóstico y Terapia, Facultad de CienciasUNAMCiudad de MéxicoMéxico

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