Biomedical Microdevices

, 17:102 | Cite as

Sub-population analysis of deformability distribution in heterogeneous red blood cell population

  • Dong Woo Lee
  • Il Doh
  • Frans A. Kuypers
  • Young-Ho Cho


We present a method for sub-population analysis of deformability distribution using single-cell microchamber array (SiCMA) technology. It is a unique method allowing the correlation of overall cellular characteristics with surface and cytosolic characteristics to define the distribution of individual cellular characteristics in heterogeneous cell populations. As a proof of principle, reticulocytes, the immature sub-population of red blood cells (RBC), were recognized from RBC population by a surface marker and different characteristics on deformability between these populations were characterized. The proposed technology can be used in a variety of applications that would benefit from the ability to measure the distribution of cellular characteristics in complex populations, especially important to define hematologic disorders.


Single cell analysis Deformability distribution Sub-population analysis Microchamber array 



This work was supported by the US National Institutes of Health grants 5R21HL092535-02, 1R24TW008822-01, and 1R43GM090515-01, and by the Converging Research Center Program funded by the Ministry of Education, Science and Technology (Project No. 2012K001442).


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Dong Woo Lee
    • 1
  • Il Doh
    • 2
  • Frans A. Kuypers
    • 3
  • Young-Ho Cho
    • 4
  1. 1.Central R&D InstituteSamsung Electro-mechanics Co., Ltd.SuwonRepublic of Korea
  2. 2.Center for Medical MetrologyKorea Research Institute of Standards and Science (KRISS)DaejeonRepublic of Korea
  3. 3.Department of HematologyChildren’s Hospital Oakland Research InstituteOaklandUSA
  4. 4.Department of Bio and Brain EngineeringKorea Advanced Institute of Science and Technology (KAIST)DaejeonRepublic of Korea

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