Sub-population analysis of deformability distribution in heterogeneous red blood cell population
We present a method for sub-population analysis of deformability distribution using single-cell microchamber array (SiCMA) technology. It is a unique method allowing the correlation of overall cellular characteristics with surface and cytosolic characteristics to define the distribution of individual cellular characteristics in heterogeneous cell populations. As a proof of principle, reticulocytes, the immature sub-population of red blood cells (RBC), were recognized from RBC population by a surface marker and different characteristics on deformability between these populations were characterized. The proposed technology can be used in a variety of applications that would benefit from the ability to measure the distribution of cellular characteristics in complex populations, especially important to define hematologic disorders.
KeywordsSingle cell analysis Deformability distribution Sub-population analysis Microchamber array
This work was supported by the US National Institutes of Health grants 5R21HL092535-02, 1R24TW008822-01, and 1R43GM090515-01, and by the Converging Research Center Program funded by the Ministry of Education, Science and Technology (Project No. 2012K001442).
- M.R. Clark, N. Mohandas, S.B. Shohet, Osmotic gradient ektacytometry: comprehensive characterization of red cell volume and surface maintenance. Blood 61, 899 (1983)Google Scholar
- I. Doh, W.C. Lee, Y. Cho, A.P. Pisano, F.A. Kuypers, Deformation measurement of individual cells in large populations using a single-cell microchamber array chip. Appl Phys Lett 173702, 10–13 (2012)Google Scholar
- A.M. Dondorp et al., Red blood cell deformability as a predictor of anemia in severe falciparum malaria. Am J Trop Med Hyg 60, 733–7 (1999)Google Scholar
- S. Shin, Y. Ku, M. Park, J. Suh, Measurement of red cell deformability and whole blood viscosity using laser-diffraction slit rheometer. Rheology 16, 85–90 (2004)Google Scholar