A comparative study of nano-scale coatings on gold electrodes for bioimpedance studies of breast cancer cells
- 454 Downloads
The relative sensitivity of standard gold microelectrodes for electric cell-substrate impedance sensing was compared with that of gold microelectrodes coated with gold nanoparticles, carbon nanotubes, or electroplated gold to introduce nano-scale roughness on the surface of the electrodes. For biological solutions, the electroplated gold coated electrodes had significantly higher sensitivity to changes in conductivity than electrodes with other coatings. In contrast, the carbon nanotube coated electrodes displayed the highest sensitivity to MDA-MB-231 metastatic breast cancer cells. There was also a significant shift in the peak frequency of the cancer cell bioimpedance signal based on the type of electrode coating. The results indicate that nano-scale coatings which introduce varying degrees of surface roughness can be used to modulate the frequency dependent sensitivity of the electrodes and optimize electrode sensitivity for different bioimpedance sensing applications.
KeywordsGold nanoparticles Carbon nanotubes Surface roughness Bioimpedance ECIS Breast cancer
We would like to thank Mr. Stephen McCartney from the Nanoscale Characterization and Fabrication Laboratory (NCFL) at the Institute for Critical Technology and Applied Science (ICTAS) for his guidance in obtaining the scanning electron microscope images shown here. We would also like to thank Mr. Hamza Shakeel for his support with electroplating the devices. This work was supported by the National Science Foundation under ECCS award number 0925945.
- M.J.P. Biggs, R.G. Richards, N. Gadegaard, R.J. McMurray, S. Affrossman, C.D.W. Wilkinson, R.O.C. Oreffo, M.J. Dalby, Interactions with nanoscale topography: adhesion quantification and signal transduction in cells of osteogenic and multipotent lineage. J. Biomed. Mater. Res. Part A 91A(1), 195–208 (2009)CrossRefGoogle Scholar
- A. Han, L. Yang, A.B.. Frazier, Quantification of the heterogeneity in breast cancer cell lines using whole-cell impedance spectroscopy. Clin. Cancer Res. 13(1), 139–143 (2007)Google Scholar