Controlled ultrasonic micro-dissection of thin tissue sections
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In order to obtain sufficient quantities of pure populations of cells or a single cell from surrounding tissue for analytical investigation, we have developed an ultrasonic microdissection system. The system utilizes a vision-based method for detecting the contact between the microdissection needle tip and a target surface. A multilayer stack piezoelectric actuator is employed to generate ultrasonic vibrations for histological isolation. Automated micro-dissection is also realized using visual feedback and vision-based control. Experimental results on tumor tissue sections show that the system has a high dissection accuracy and efficiency and is able to realize dissecting arbitrary shapes in specified locations on a tissue sample. Furthermore, effects in variations of vibration amplitude and frequency of ultrasonic micro-dissection as well as needle insertion depths on micro-dissection accuracy and speed were evaluated.
KeywordsPiezoelectric actuator Ultrasonic vibration Depth control Tissue microdissection Parameter selection
This work was financially supported by National Natural Science of China (Grant No. 61233010), Instrument Development Major Program of National Natural Science of China (Grant No. 61327811), Jiangsu Natural Science Founds for Distinguished Young Scholar (Grant No. BK2012005), and Qing Lan Project of Jiangsu Province.