A self-contained, programmable microfluidic cell culture system with real-time microscopy access
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Utilizing microfluidics is a promising way for increasing the throughput and automation of cell biology research. We present a complete self-contained system for automated cell culture and experiments with real-time optical read-out. The system offers a high degree of user-friendliness, stability due to simple construction principles and compactness for integration with standard instruments. Furthermore, the self-contained system is highly portable enabling transfer between work stations such as laminar flow benches, incubators and microscopes. Accommodation of 24 individual inlet channels enables the system to perform parallel, programmable and multiconditional assays on a single chip. A modular approach provides system versatility and allows many different chips to be used dependent upon application. We validate the system’s performance by demonstrating on-chip passive switching and mixing by peristaltically driven flows. Applicability for biological assays is demonstrated by on-chip cell culture including on-chip transfection and temporally programmable gene expression.
KeywordsProgrammable Cell culture Microfluidic Portable User-friendly Modular
This work was supported by Grant No. 2106-08-0018 “ProCell”, under the Programme Commission on Strategic Growth Technologies, the Danish Agency for Science, Technology and Innovation.
- A.J. Conde, D. Sabourin, P. Skafte-Pedersen and M. Dufva, Proceedings of the 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences (uTAS2011), 2–6 October 2011, Seattle, Washington, USA, eds. J.P. Landers, A. Herr, D. Juncker, N. Pamme, and J. Bienvenue, (CBMS, 2011) pp. 1621-1623 (2011)Google Scholar
- M. Hemmingsen, P. Skafte-Pedersen, D. Sabourin, R.F. Andersen, A.L. Sørensen, P. Collas and M. Dufva, Proceedings of the 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences (uTAS2011), 2–6 October 2011, Seattle, Washington, USA, eds. J.P. Landers, A. Herr, D. Juncker, N. Pamme, and J. Bienvenue, (CBMS, 2011) pp. 834–836 (2011)Google Scholar
- Y.-K. Lee, P. Tabeling, C. Shih and C.-M. Ho, Proceedings of the ASME International Mechanical Engineering Congress and Exposition, November 5–10, 2000, Orlando, Florida, USA, pp. 505–511 (2000)Google Scholar
- S. Lindström, M. Eriksson, T. Vazin, J. Sandberg, J. Lundeberg, J. Frisén and H. Andersson-Svahn, PLOS ONE 4, -, (2009)Google Scholar
- M. Liu and Y.-C. Tai, Biomedical Microdevices, 1–11, (2010)Google Scholar
- D. Sabourin, D. Snakenborg, M. Dufva, Microfluidics and Nanofluidics 9, 87–93 (2010a)Google Scholar
- D. Sabourin, P. Skafte-Pedersen, V. Coman, M. Hemmingsen, J. Petersen, J.P. Kutter, J. Emneus, D. Snakenborg and M. Dufva, Proceedings of the 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences (uTAS2010), 3–7 October 2010, Groningen, The Netherlands, eds. S. Verporte, H. Andersson, J. Emneus, and N. Pamme (CBMS, 2010) pp. 166–168 (2010b)Google Scholar
- J.H. Yeon, J.-K. Park, Biochip Journal 1, 17–27 (2007)Google Scholar