Abstract
This paper describes a vacuum-accelerated microfluidic immunoassay (we abbreviate it as VAMI) by sandwiching a filter membrane between a two-layer chip. A direct assay of IgG demonstrated that VAMI could simultaneously achieve higher sensitivity and require less time compared with conventional microfluidic immunoassays. We further applied VAMI to carry out a 3-step competitive assay (including antigen immobilization, competitive reaction and 2nd antibody reaction) for detecting the illegal food additive Sudan Red. A total assay time of 15 min with a limit of detection (LOD) of 1 ng ml-1 is achieved.
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Acknowledgment
We thank National Science Foundation of China (51073045, 21025520, 20890020, 90813032, 21105018), the Ministry of Science and Technology (2009CB930001, 2007CB714502,2011CB933201), Chinese Academy of Science (KJCX2-YW-M15) and Beijing Entry-Exit Inspection and Quarantine Bureau(2010IK267) to support for this work.
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Liu, Y., Yu, J., Du, M. et al. Accelerating microfluidic immunoassays on filter membranes by applying vacuum. Biomed Microdevices 14, 17–23 (2012). https://doi.org/10.1007/s10544-011-9581-z
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DOI: https://doi.org/10.1007/s10544-011-9581-z