Accelerating microfluidic immunoassays on filter membranes by applying vacuum
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This paper describes a vacuum-accelerated microfluidic immunoassay (we abbreviate it as VAMI) by sandwiching a filter membrane between a two-layer chip. A direct assay of IgG demonstrated that VAMI could simultaneously achieve higher sensitivity and require less time compared with conventional microfluidic immunoassays. We further applied VAMI to carry out a 3-step competitive assay (including antigen immobilization, competitive reaction and 2nd antibody reaction) for detecting the illegal food additive Sudan Red. A total assay time of 15 min with a limit of detection (LOD) of 1 ng ml-1 is achieved.
KeywordsMicrofluidics Cross array Immunoassay Vacuum Accelerating
We thank National Science Foundation of China (51073045, 21025520, 20890020, 90813032, 21105018), the Ministry of Science and Technology (2009CB930001, 2007CB714502,2011CB933201), Chinese Academy of Science (KJCX2-YW-M15) and Beijing Entry-Exit Inspection and Quarantine Bureau(2010IK267) to support for this work.