Abstract
Alternatives for ossicular replacements were fabricated in order to overcome persisting rejections in middle ear prosthetization. Unlike the synthetic prostheses in fashion, we propose biological and biohybrid replacements containing extra cellular matrix (ECM) molecules to improve biointegration. In this study, ECM-containing devices shaped as Partial Ossicular Replacement Prostheses (PORPs) were fabricated reproducing the current synthetic models. Biological PORPs were obtained from human decellularized cortical bone allografts by computer numerically controlled ultraprecision micromilling. Moreover, porous PORP-like scaffolds were produced and cultured with osteoinduced human mesenchymal stromal cells to generate in vitro bone ECM within the scaffold porosity (biohybrid PORPs). The acoustic responses of such devices were investigated and compared to those of commercial prostheses. Results showed that biological PORPs transmit mechanical signals with appropriate frequencies, amplitudes, and with early extinction time. Although signal transmission in biohybrid PORPs showed insufficient amplitude, we believe that tissue engineered constructs represent the new challenge in ossiculoplasty.
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Acknowledgements
Regione Toscana (Italy) and Center for Excellence ENDOCAS (University of Pisa) (MIUR D.M. 17 Oct 2003, 193/2003) are gratefully acknowledged for funding this study. The authors wish to thank Dr. Antonios G. Mikos (Rice University, Houston, TX) and his research team for the technical support in scaffold fabrication and analysis. Many thanks are also due to Mr. Nicodemo Funaro (CRIM Lab, Scuola Superiore Sant’Anna, Pisa, Italy) for his fundamental contribution in allograft PORP fabrication. Paper number 003 of CUCCS-RRMR.
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Danti, S., Stefanini, C., D’Alessandro, D. et al. Novel biological/biohybrid prostheses for the ossicular chain: fabrication feasibility and preliminary functional characterization. Biomed Microdevices 11, 783–793 (2009). https://doi.org/10.1007/s10544-009-9293-9
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DOI: https://doi.org/10.1007/s10544-009-9293-9