Nano-opto-mechanical characterization of neuron membrane mechanics under cellular growth and differentiation
- 258 Downloads
We designed and fabricated silicon probe with nanophotonic force sensor to directly stimulate neurons (PC12) and measured its effect on neurite initiation and elongation. A single-layer pitch-variable diffractive nanogratings was fabricated on silicon nitride probe using e-beam lithography, reactive ion etching and wet-etching techniques. The nanogratings consist of flexure folding beams suspended between two parallel cantilevers of known stiffness. The probe displacement, therefore the force, can be measured through grating transmission spectrum. We measured the mechanical membrane characteristics of PC12 cells using the force sensors with displacement range of 10 μm and force sensitivity 8 μN/μm. Young’s moduli of 425 ± 30 Pa are measured with membrane deflection of 1% for PC12 cells cultured on polydimethylsiloxane (PDMS) substrate coated with collagen or laminin in Ham’s F-12K medium. In a series of measurements, we have also observed stimulation of directed neurite contraction up to 6 μm on extended probing for a time period of 30 min. This method is applicable to measure central neurons mechanics under subtle tensions for studies on development and morphogenesis. The close synergy between the nano-photonic measurements and neurological verification can improve our understanding of the effect of external conditions on the mechanical properties of cells during growth and differentiation.
KeywordsMechanotransduction Cytomechanics PC12 Cell membrane Growth Differentiation Nanogratings Micro-electro-mechanical systems (MEMS) Force sensor
We would like to thank Microelectronics Research Center (MRC) and the Center for Nano and Molecular Science and Technology (CNM) at UT Austin for providing the microfabrication facilities. This study was supported in part by the National Science Foundation Nanoscale Exploratory Research Program (ECS-0609413).
- A.R. Bausch, F. Ziemann et al., Biophys. J. 75, 2038–2049 (1998)Google Scholar
- D.E. Discher, P. Janmey, et al., 310, 1139–1143 (2005)Google Scholar
- J.W. Goodman, Introduction to Fourier optics (Roberts & Co 2005)Google Scholar
- A. Gopal, Z. Luo, et al., Solid-state sensors, actuators and microsystems conference, 2007. TRANSDUCERS 2007. International, 1239–1242 (2007)Google Scholar
- J. Guck, R. Ananthakrishnan et al., Biophys. J. 81, 767–784 (2001)Google Scholar
- A.I. Lur’e Three-dimensional problems of the theory of elasticity (Interscience Publishers 1964)Google Scholar
- V. Nesterov, U. Brand. The nonlinear mechanical and elastrical properties of silicon 3D micro probes, euspen. (2004)Google Scholar
- M. Radmacher, M. Fritz et al., Biophys. J. 70, 556–567 (1996)Google Scholar
- S.D. Senturia Microsystem Design (Kluwer Academic Publishers 2001)Google Scholar
- X. Zhang, M.P. Scott, et al., J. Microelectro. Mech. Syst. 15 (2006)Google Scholar
- J. Zheng, P. Lamoureux et al., J. Neurosci. 11, 1117–1125 (1991)Google Scholar