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Transcriptome analysis device based on liquid phase detection by fluorescently labeled nucleic acid probes

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Abstract

Personalized medicine based on genetic information has been proposed as an attractive medical treatment. It is supported by the rapid development of the gene diagnosis utilizing on-chip analysis technology. This study reports characterizations of mRNA detection device by its hybridization with 2′-O-methyl oligoribonucleotide probe in a liquid phase, which eliminates time-consuming processes such as removing non-hybridized probes in conventional solid phase detection. In order to achieve a high sensitivity in the fluorescent detection of the target mRNA, we have optimized detection channel depth and width for a microfluidic device, and investigated polydimethyl siloxiane components and observation setup to minimize background noise. Adopting optimized channel dimensions and setup for the probe, the fluorescent intensity increased 3.3-fold at the lowest detectable concentration of 7.8 nM.

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Acknowledgements

PDMS components were supplied by Shin-Etsu Chemical Co., Ltd. This work was carried out under the 21st century COE program “Micro/Nano Science and Integrated Systems” supported by the Ministry of Education and Kyoto nanotechnology cluster supported by the Advanced Software Technology and Mechatronics Research Institute.

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Authors and Affiliations

  1. Department of Microsystem Technology, Ritsumeikan University, 1-1-1 Noji-higashi, Kusatsu, Shiga, Japan

    Ryuji Yokokawa, Soichiro Tamaoki & Susumu Sugiyama

  2. Department of Chemistry and Materials Technology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto, 606-8585, Japan

    Takashi Sakamoto & Akira Murakami

Authors
  1. Ryuji Yokokawa
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  2. Soichiro Tamaoki
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  3. Takashi Sakamoto
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  4. Akira Murakami
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  5. Susumu Sugiyama
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Correspondence to Ryuji Yokokawa.

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Yokokawa, R., Tamaoki, S., Sakamoto, T. et al. Transcriptome analysis device based on liquid phase detection by fluorescently labeled nucleic acid probes. Biomed Microdevices 9, 869–875 (2007). https://doi.org/10.1007/s10544-007-9100-4

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  • DOI: https://doi.org/10.1007/s10544-007-9100-4

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