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Biochemistry (Moscow)

, Volume 70, Issue 11, pp 1268–1273 | Cite as

Effects of Oxygen, Heavy Water, and Glycerol on Electron Transfer in the Acceptor Part of Rhodobacter sphaeroides Reaction Centers

  • P. P. Knox
  • M. S. Baptista
  • A. F. Uchoa
  • N. I. Zakharova
Article

Abstract

The kinetics of electron transfer between primary and secondary quinone acceptors of the photosynthetic reaction center (RC) of the purple bacterium Rhodobacter sphaeroides wild type was studied at the wavelengths 400 and 450 nm. It was shown that removing of molecular oxygen from RC preparations slowed down the fast phase of the process from 4–4.5 µsec to tens of microseconds. Similar effects were observed after the incubation of RC in heavy water for 72 h or glycerol addition (90% v/v) to RC preparations. The observed effects are interpreted in terms of the influence of these agents on the hydrogen bond system of the RC. The state of this system can determine the formation of different RC conformations that are characterized by different rates of electron transfer between quinone acceptors.

Key words

purple bacteria photosynthetic reaction center quinone acceptors electron transport oxygen heavy water glycerol 

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REFERENCES

  1. 1.
    Knox, P. P., Lukashev, E. P., Timofeev, K. N., and Seifullina, N. Kh. (2002) Biochemistry (Moscow), 67, 901–907.CrossRefGoogle Scholar
  2. 2.
    Okamura, M. Y., and Feher, G. (1992) Annu. Rev. Biochem., 61, 861–896.CrossRefPubMedGoogle Scholar
  3. 3.
    Lancaster, C. R. D., Michel, H., Honig, B., and Gunner, M. R. (1996) Biophys. J., 70, 2469–2492.PubMedGoogle Scholar
  4. 4.
    Miksovska, J., Maroti, P., Tandori, J., Schiffer, M., Hanson, D. K., and Sebban, P. (1996) Biochemistry, 35, 15411–15417.CrossRefPubMedGoogle Scholar
  5. 5.
    Abgaryan, G. A., Christophorov, L. N., Goushcha, A. O., Holzwarth, A. R., Kharkyanen, V. N., Knox, P. P., and Lukashev, E. P. (1998) J. Biol. Phys., 24, 1–17.CrossRefGoogle Scholar
  6. 6.
    Goushcha, A. O., Manzo, A. J., Scott, G. W., Christophorov, L. N., Knox, P. P., Barabash, Yu. M., Kapustina, M. T., Berezetska, N. M., and Kharkyanen, V. N. (2003) Biophys. J., 84, 1146–1160.PubMedGoogle Scholar
  7. 7.
    Okamura, M. Y., Paddock, M. L., Graige, M. S., and Feher, G. (2000) Biochim. Biophys. Acta, 1458, 148–163.PubMedGoogle Scholar
  8. 8.
    Pimenova, M. N., Grechushkina, N. N., Azova, L. G., Semenova, E. V., and Myl'nikova, S. I. (1983) Practical Handbook of Microbiology [in Russian], MGU Publishers, Moscow.Google Scholar
  9. 9.
    Zakharova, N. I., and Churbanova, I. Yu. (2000) Biochemistry (Moscow), 65, 149–159.Google Scholar
  10. 10.
    Clayton, R. K. (1981) Photosynthesis: Physical Mechanisms and Chemical Patterns, Cambridge University Press, London-New York.Google Scholar
  11. 11.
    Parson, W. W. (1969) Biochim. Biophys. Acta, 189, 384–396.PubMedGoogle Scholar
  12. 12.
    Wraight, C. A. (1979) Biochim. Biophys. Acta, 548, 309–327.PubMedGoogle Scholar
  13. 13.
    Tiede, D. M., Vazquez, J., Cordova, J., and Marone, P. A. (1996) Biochemistry, 35, 10763–10775.CrossRefPubMedGoogle Scholar
  14. 14.
    Bensasson, R., and Land, E. J. (1973) Biochim. Biophys. Acta, 325, 175–181.PubMedGoogle Scholar
  15. 15.
    Shinkarev, V. P., and Wraight, C. A. (1993) in The Photosynthetic Reaction Center, Vol. 1 (Deisenhofer, J., and Norris, J., eds.) Academic Press, San Diego, pp. 193–255.Google Scholar
  16. 16.
    Li, J., Gilroy, D., Tiede, D. M., and Gunner, M. R. (1998) Biochemistry, 37, 2818–2829.PubMedGoogle Scholar
  17. 17.
    Tiede, D. M., Gallo, D. M., and Hanson, D. K. (1997) Biophys. J., 72, A248.Google Scholar
  18. 18.
    Hienerwadel, R., Grzybek, S., Fogel, C., Kreutz, W., Okamura, M. Y., Paddock, M. L., Breton, J., Nabedryk, E., and Mantele, W. (1995) Biochemistry, 34, 2832–2843.PubMedGoogle Scholar
  19. 19.
    Scheiner, S., and Hillenbrand, E. A. (1985) Proc. Natl. Acad. Sci. USA, 82, 2741–2745.PubMedGoogle Scholar
  20. 20.
    Tandori, J., Hideg, E., Nagy, L., Maroti, P., and Vass, I. (2001) Photosynth. Res., 70, 175–184.CrossRefPubMedGoogle Scholar
  21. 21.
    Cioni, P., and Strambini, B. (2002) Biophys. J., 82, 3246–3253.PubMedCrossRefGoogle Scholar
  22. 22.
    Lobyshev, V. I., and Kalinichenko, L. P. (1978) Isotope Effects of D 2 O in Biological Systems [in Russian], Nauka, Moscow.Google Scholar
  23. 23.
    Scheiner, S., and Cuma, M. (1996) J. Am. Chem. Soc., 118, 1511–1521.CrossRefGoogle Scholar
  24. 24.
    Paschenko, V. Z., Korvatovskii, B. N., Logunov, S. L., Kononenko, A. A., Knox, P. P., Zakharova, N. I., Grishanova, N. P., and Rubin, A. B. (1987) FEBS Lett., 214, 28–34.CrossRefGoogle Scholar
  25. 25.
    Paschenko, V. Z., Gorokhov, V. V., Grishanova, N. P., Goryacheva, E. A., Korvatovsky, B. N., Knox, P. P., Zakharova, N. I., and Rubin, A. B. (1998) Biochim. Biophys. Acta, 1364, 361–372.PubMedGoogle Scholar
  26. 26.
    Paschenko, V. Z., Knox, P. P., Chamorovsky, S. K., Krasilnikov, P. M., Mamedov, M. D., Semenov, A. Yu., Zakharova, N. I., Renger, G., and Rubin, A. B. (2001) Bioelectrochemistry, 53/2, 233–241.CrossRefPubMedGoogle Scholar
  27. 27.
    Knox, P. P., Kononenko, A. A., and Rubin, A. B. (1980) Biofizika, 25, 239–241.Google Scholar

Copyright information

© MAIK "Nauka/Interperiodica" 2005

Authors and Affiliations

  • P. P. Knox
    • 1
  • M. S. Baptista
    • 2
  • A. F. Uchoa
    • 2
  • N. I. Zakharova
    • 1
  1. 1.Biology FacultyLomonosov Moscow State UniversityMoscowRussia
  2. 2.Departamento de Bioquimica, Instituto de QuimicaUniversidade de Sao Paulo, 748Sao PauloBrazil

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