Biochemistry (Moscow)

, Volume 70, Issue 10, pp 1095–1103 | Cite as

Effect of “External” Superoxide Anion on Apoptosis in Coleoptiles of Wheat Seedlings

  • A. A. Vorobjev
  • E. G. Smirnova
  • L. E. Bakeeva
  • L. S. Yaguzhinsky


A derivative of phthalic acid, dibutylphthalate (DBP), which has gametocidal effect at the concentration of ∼10−4 M, increased apoptosis in coleoptiles of wheat seedlings. This was associated with activation of chromatin margination and generation of mitochondria-containing vesicles. At the same concentration, DBP activated the release by the coleoptiles of superoxide anion into the environment. Lower (10−5 M) and higher (10− 3 M) concentrations of DBP virtually had no effect on either process. A probable mechanism of effect of the “ external” superoxide anion on apoptosis within the plant cell is discussed.

Key words

wheat seedlings apoptosis superoxide anion mitochondrial ultrastructure 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Jabs, T. (1999) Biochem. Pharmacol., 57, 231–245.PubMedCrossRefGoogle Scholar
  2. 2.
    Frahry, G., and Schopfer, P. (2001) Planta, 212, 175–183.PubMedCrossRefGoogle Scholar
  3. 3.
    Mahalingam, R., and Fedoroff, N. (2003) Physiol. Plant., 119, 56–68.CrossRefGoogle Scholar
  4. 4.
    Bakeeva, L. E., Kirnos, M. D., Aleksandrushkina, N. I., Kazimirchyuk, S. V., Shorning, B. Yu., Zamyatnina, V. A., Yaguzhinsky, L. S., and Vanyushin, B. F. (1999) FEBS Lett., 457, 122–125.PubMedCrossRefGoogle Scholar
  5. 5.
    Shorning, B. Yu., Smirnova, E. G., Yaguzinsky, L. S., and Vanyushin, B. F. (2000) Biochemistry (Moscow), 65, 1357–1361.CrossRefGoogle Scholar
  6. 6.
    Auclair, C., and Voisin, E. (1987) in CRC Handbook of Methods for Oxygen Radical Research (Greenwald, R. A., ed.) CRC Press, Inc., Boca Ration, Florida, pp. 123–132.Google Scholar
  7. 7.
    Smirnova, E. G., Lyubimov, Yu. I., Malinina, T. G., Lyubimova, E. Yu., Aleksandrushkina, N. I., Vanyushin, B. F., Kolesova, G. M., and Yaguzhinsky, L. S. (2002) Biochemistry (Moscow), 67, 1271–1275.CrossRefGoogle Scholar
  8. 8.
    Reynolds, E. (1963) J. Cell Biol., 17, 208–212.PubMedCrossRefGoogle Scholar
  9. 9.
    Turrens, J. F., and Boveris, A. (1980) Biochem. J., 191, 421–427.PubMedGoogle Scholar
  10. 10.
    Aleksandrushkina, N. I., Zamyatnina, V. A., Bakeeva, L. E., Seredina, A. V., Smirnova, E. G., Yaguzhinsky, L. S., and Vanyushin, B. F. (2004) Biochemistry (Moscow), 69, 285–294.CrossRefGoogle Scholar
  11. 11.
    Bakeeva, L. E., Zamyatnina, V. A., Shorning, B. Yu., Aleksandrushkina, N. I., and Vanyushin, B. F. (2001) Biochemistry (Moscow), 66, 850–859.Google Scholar
  12. 12.
    Lecourieux, D., Mazars, C., Pauly, N., Ranjeva, R., and Pugin, A. (2002) Plant Cell, 14, 2627–2641.PubMedCrossRefGoogle Scholar
  13. 13.
    Hara, Y., Wakamori, M., Ishii, M., Maeno, E., Nishida, M., Yoshida, T., Yamada, H., Shimizu, S., Mori, E., Kudoh, J., Shimizu, N., Kurose, H., Okada, Y., Imoto, K., and Mori, Y. (2002) Mol. Cell., 9, 163–173.PubMedCrossRefGoogle Scholar
  14. 14.
    Banfi, B., Tirone, F., Durussel, I., Knisz, J., Moskwa, P., Molnar, G. Z., Krause, K.-H., and Cox, J. A. (2004) J. Biol. Chem., 279, 18583–18591.PubMedGoogle Scholar
  15. 15.
    Tassani, V., Biban, C., Toninello, A., and Siliprandi, D. (1995) Biochem. Biophys. Res. Commun., 207, 661–667.PubMedCrossRefGoogle Scholar
  16. 16.
    Castilho, R. F., Kowaltowski, A. J., Meinicke, A. R., Bechara, E. J. H., and Vercesi, A. E. (1995) Free Rad. Biol. Med., 18, 479–486.PubMedGoogle Scholar
  17. 17.
    Li, P., Nijhawan, D., Budihardjo, I., Srinivasula, S. M., Ahmad, M., Alnemri, E. S., and Wang, X. (1997) Cell, 91, 479–489.PubMedCrossRefGoogle Scholar

Copyright information

© MAIK "Nauka/Interperiodica" 2005

Authors and Affiliations

  • A. A. Vorobjev
    • 1
  • E. G. Smirnova
    • 2
  • L. E. Bakeeva
    • 1
  • L. S. Yaguzhinsky
    • 1
  1. 1.Belozersky Institute of Physico-Chemical BiologyLomonosov Moscow State UniversityMoscowRussia
  2. 2.Institute of Agricultural BiotechnologyRussian Academy of Agricultural SciencesMoscowRussia

Personalised recommendations