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Biochemistry (Moscow)

, Volume 70, Issue 7, pp 822–832 | Cite as

Spectral Study of Interactions of 4,8,4′-Trimethylpsoralen and 4,4′-Dimethylangelicin Dyes with DNA

  • V. S. Sibirtsev
  • A. Yu. Tolmachev
  • M. V. Kovaleva
  • A. V. Garabadzhiu
  • V. F. Traven
Article

Abstract

Absorption and fluorescence spectra for six new synthetic dyes of 4,8,4′-trimethylpsoralen and 4,4′-dimethylangelicin derivatives containing various terminal substituents at 5′-position have been investigated in different environments using a wide range of the DNA/ligand concentrations. Various spectral and binding characteristics of the DNA-ligand systems have been determined. General principles characterizing mechanisms responsible for changes in the fluorescent properties of nucleotide-specific dyes have been proposed; they take into consideration chemical structure of the dyes, properties of the environment, and degree of sorption on substrate.

Key words

DNA fluorescence dyes spectral and binding properties 

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Copyright information

© MAIK “Nauka/Interperiodica” 2005

Authors and Affiliations

  • V. S. Sibirtsev
    • 1
  • A. Yu. Tolmachev
    • 2
  • M. V. Kovaleva
    • 3
  • A. V. Garabadzhiu
    • 3
  • V. F. Traven
    • 2
  1. 1.Mendeleyev Russian Research Institute for MetrologySt. PetersburgRussia
  2. 2.Mendeleyev University of Chemical Technology of RussiaMoscowRussia
  3. 3.St. Petersburg State Technological InstituteSt. PetersburgRussia

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