Skip to main content
SpringerLink
Log in

Comparative Analysis of Transcription Profiles of Helicobacter pylori Clinical Isolates

  • Accelerated Publication
  • Published:
Biochemistry (Moscow) Aims and scope Submit manuscript

Abstract

The transcription profiles of four Helicobacter pylori clinical isolates (two cag-negative and two cag-positive) were compared in stationary growth phase using a cDNA-macroarray. The correlation coefficient value between total transcription profiles of clinical isolates H. pylori varied from 0.70 to 0.83. For 44 groups of genes (total number 66) belonging to various functional classes of H. pylori, the correlation coefficient value between these isolates exceeded 0.7, and for 14 groups the value exceeded 0.9. These groups included genes encoding components involved in cell division, adaptations to atypical conditions, electron transport, salvage of nucleosides and nucleotides, glycolysis/gluconeogenesis, folding and stabilization of proteins, translation factors, anaerobic metabolism, and amino acids and amine metabolism. Expression of 52 genes significantly differed between H. pylori clinical isolates. Some of these genes determine microorganism virulence. They include: cytotoxin-associated gene (cagA), genes encoding neutrophil-activating protein (napA), major flagellar protein (flaA), and vacuolizing cytotoxin (vacA), some genes encoding outer membrane proteins (omp), urease alpha and beta subunits (ureA and ureB), and some regulatory proteins, and genes encoding stress-related proteins, such as the chaperone and heat shock protein genes (groEL and dnaK).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

REFERENCES

  1. Sherbakov, P. L. (1999) in Helicobacter pylori: Revolution in Gastroenterology (Ivashkin, V. T., Megro, F., and Lapina, T. L., eds.) [in Russian], Triada-X, Moscow.

    Google Scholar 

  2. Poundeyr, R. E., and Ng, D. (1995) Aliment. Pharmacol. Ther., 9, S33–S39.

    Google Scholar 

  3. Pretolani, S., Bonvicini, R., and Gasbarrini, G. (1997) in Helicobacter pylori. An Atlas (Malrertheiner, P., Michetti, P., and Price, A., eds.) Science Press Limited, London, pp. 2.1–2.6.

    Google Scholar 

  4. Blaser, M. J., Perez-Perez, G. I., Kleanthous, H., Cover, T. L., et al. (1995) Cancer Res., 55, 2111–2115.

    CAS  PubMed  Google Scholar 

  5. Nomura, A., Stemmermann, G. N., Chyou, P. H., Kato, I., et al. (1991) New Engl. J. Med., 325, 1132–1136.

    CAS  PubMed  Google Scholar 

  6. Parsonnet, J., Friedman, G. D., Vandersteen, D. P., Chang, Y., et al. (1991) New Engl. J.Med., 325, 1127–1131.

    CAS  PubMed  Google Scholar 

  7. Peterson, W. L. (1991) New Engl. J. Med., 324, 1043–1048.

    CAS  PubMed  Google Scholar 

  8. Gerhard, M., Rad, R., Prinz, C., and Naumann, M. (2002) Helicobacter, 7, S17–S23.

    Google Scholar 

  9. Covacci, A., Telford, J. L., Del Giudice, G., Parsonnet, J., et al. (1999) Science, 284, 1328–1333.

    CAS  PubMed  Google Scholar 

  10. Blaser, M. J. (1996) Aliment. Pharmacol. Ther., 10, S73–S77.

    Google Scholar 

  11. Atherton, J. C., Cao, P., Peek, R. M. J., Tummuru, M. K., et al. (1995) J. Biol. Chem., 270, 17771–17777.

    CAS  PubMed  Google Scholar 

  12. Peek, R., Thompson, S., Donahue, J., Tham, K., et al. (1998) Proc. Assos. Am. Physicians, 110, 58–66.

    Google Scholar 

  13. Ilver, D., Arnquist, A., Ogren, J., Frick, I., et al. (1998) Science, 279, 373–377.

    Article  CAS  PubMed  Google Scholar 

  14. Xiang, Z., Censini, S., Bayeli, P. F., Telford, J. L., et al. (1995) Infect. Immun., 63, 94–98.

    CAS  PubMed  Google Scholar 

  15. Gerhard, M., Lehn, N., Neumayer, N., Boren, T., et al. (1999) Proc. Natl. Acad. Sci. USA, 96, 12778–12783.

    CAS  PubMed  Google Scholar 

  16. Alm, R. A., and Trust, T. J. (1999) Mol. Med., 77, 834–846.

    CAS  Google Scholar 

  17. Tomb, J. F., White, O., Kerlavage, A. R., et al. (1997) Nature, 388, 539–547.

    Article  CAS  PubMed  Google Scholar 

  18. Alm, R. A., Ling, L. S. L., Moir, D. T., et al. (1999) Nature, 397, 176–180.

    Article  PubMed  Google Scholar 

  19. Salama, N., Guillemin, K., McDaniel, T. K., Sherlock, G., et al. (2000) Proc. Natl. Acad. Sci. USA, 97, 14668–14673.

    CAS  PubMed  Google Scholar 

  20. Momynaliev, K. T., Smirnova, O. V., Kudryavtseva, L. V., and Govorun, V. M. (2003) Mol. Biol. (Moscow), 37, 625–633.

    CAS  Google Scholar 

  21. Chanto, G., Occhialini, A., Gras, N., Alm, R., et al. (2002) Microbiology, 148, 3671–3680.

    CAS  PubMed  Google Scholar 

  22. Ang, S., Lee, C. Z., Peck, K., Sindici, M., Matrubutham, U., Gleeson, M. A., and Wang, J. T. (2001) Infect. Immun., 69, 1679–1686.

    CAS  PubMed  Google Scholar 

  23. Wen, Y., Marcus, E. A., Matrubutham, U., Gleeson, M. A., Scott, D. R., and Sachs, G. (2003) Infect. Immun., 71, 5921–5939.

    CAS  PubMed  Google Scholar 

  24. Allan, E., Clayton, C. L., McLaren, A., Wallace, D. M., and Wren, B. W. (2001) Microbiology, 147, 2285–2292.

    CAS  PubMed  Google Scholar 

  25. Niehus, E., Gressmann, H., Ye, F., Schlapbach, R., Dehio, M., Dehio, C., Stack, A., Meyer, T. F., Suerbaum, S., and Josenhans, C. (2004) Mol. Microbiol., 52, 947–961.

    CAS  PubMed  Google Scholar 

  26. Contreras, M., Thiberge, J. M., Mandrand-Berthelot, M. A., and Labigne, A. (2003) Mol. Microbiol., 49, 947–963.

    CAS  PubMed  Google Scholar 

  27. Kim, N., Marcus, E. A., Wen, Y., Weeks, D. L., Scott, D. R., Jung, H. C., Song, I. S., and Sachs, G. (2004) Infect. Immun., 72, 2358–2368.

    CAS  PubMed  Google Scholar 

  28. Josenhans, C., Niehus, E., Amersbach, S., Horster, A., Betz, C., Drescher, B., Hughes, K. T., and Suerbaum, S. (2002) Mol. Microbiol., 43, 307–322.

    CAS  PubMed  Google Scholar 

  29. Thompson, L. J., Merrell, D. S., Neilan, A., Mitchell, H., Lee, A., and Falkow, S. (2003) Infect. Immun., 71, 2643–2655.

    CAS  PubMed  Google Scholar 

  30. Marais, A., Mendz, G. L., Hazell, S. L., and Megraud, F. (1999) Microb. Mol. Biol. Rev., 63, 642–674.

    CAS  Google Scholar 

  31. Taylor, D. E., Eaton, M., Chang, N., and Salama, S. M. (1992) J. Bacteriol., 174, 6800–6806.

    CAS  PubMed  Google Scholar 

  32. Van Doorn, N. E., Namavar, F., Kusters, J. G., van Rees, E. P., et al. (1998) FEMS Microbiol. Lett., 160, 145–150.

    PubMed  Google Scholar 

  33. Marshall, D. G., Coleman, D. C., Sullivan, D. J., Xia, H., et al. (1996) J. Appl. Bacteriol., 81, 509–517.

    CAS  PubMed  Google Scholar 

  34. Akopyants, N., Bukanov, N. O., Westblom, T. U., and Berg, D. E. (1992) Nucleic Acids Res., 20, 6221–6225.

    PubMed  Google Scholar 

  35. Akopyants, N., Fradkov, A., Diatchenko, L., Hill, J., et al. (1998) Proc. Natl. Acad. Sci. USA, 95, 13108–13113.

    CAS  PubMed  Google Scholar 

  36. Marshall, D. G., Dundon, W. G., Beesley, S. M., and Smyth, C. J. (1998) Microbiology, 144, 2925–2939.

    CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Research Institute for Physico-Chemical Medicine, ul. Malaya Pirogovskaya 1a, 119992, Moscow, Russia

    K. T. Momynaliev, S. I. Rogov, O. V. Selezneva, V. V. Chelysheva, T. A. Akopian & V. M. Govorun

Authors
  1. K. T. Momynaliev
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. I. Rogov
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. O. V. Selezneva
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. V. V. Chelysheva
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. T. A. Akopian
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. V. M. Govorun
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to K. T. Momynaliev.

Additional information

__________

Translated from Biokhimiya, Vol. 70, No. 4, 2005, pp. 467–475.

Original Russian Text Copyright © 2005 by Momynaliev, Rogov, Selezneva, Chelysheva, Akopian, Govorun.

Originally published in Biochemistry (Moscow) On-Line Papers in Press, as Manuscript BM04-268, March 27, 2005.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Momynaliev, K.T., Rogov, S.I., Selezneva, O.V. et al. Comparative Analysis of Transcription Profiles of Helicobacter pylori Clinical Isolates. Biochemistry (Moscow) 70, 383–390 (2005). https://doi.org/10.1007/s10541-005-0129-9

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10541-005-0129-9

Key words

Search

Navigation