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Bioscience Reports

, Volume 26, Issue 1, pp 39–43 | Cite as

Antigenic Profile of Osteoblasts Present in Human Bone Tissue Sections

  • O.  García-Martínez
  • C. Reyes-Botella
  • O. Aguilera-Castillo
  • M.F. Vallecillo-Capilla
  • C. Ruiz
Article

Abstract

The antigenic profile of human osteoblasts was previously analyzed by our group using primary cultures as study samples. These studies suggested a novel functional approach to this cell population. Osteoblasts have a characteristic antigenic profile and share antigens in common with other cell populations that also originate in the bone marrow. Some of the detected antigens are constitutively expressed, while others are modulated by different factors and/or cytokines. The aim of the present study was to analyze the antigens present in osteoblasts in vivo, since the presence of certain biomolecules in fetal bovine serum may modulate the antigenic expression, compromising the results. For this purpose, human bone tissue sections were analyzed with a wide panel of mAbs and using the immunoperoxidase technique. CD10, CD44 and alkaline phosphatase antigens and IL-12, IL-18 and IFNγ cytokines were detected in osteoblasts in the bone tissue. However, CD80 and HLA-DR antigens were not found in all samples and when present their expression was weak. The expression of CD54 antigen was moderate or weak. These results allow data obtained by the primary culture of osteoblast-like cells to be endorsed.

Keywords

Osteoblasts Bone tissue Antigenic phenotype Immunohistochemistry 

References

  1. 1.
    Aubin JE, Liu F, Malaval L, Gupta AK (1995) Osteoblast and chondroblast differentiation. Bone 17:17S–83SCrossRefGoogle Scholar
  2. 2.
    Ducy P, Schinke T, Karsenty G (2000) The osteoblast: a sophisticated fibroblast under central surveillance. Science 289:1501–1504CrossRefPubMedGoogle Scholar
  3. 3.
    Canalis E, Delany AM (2002) Mechanisms of glucocorticoid action in bone. Ann NY Acad Sci 966:73–81PubMedCrossRefGoogle Scholar
  4. 4.
    Kessler S, Katler S, Mayr-Wohfart U, Puhl W, Gunther KP (2000) Stimulation of primary cultures with rh-TGFβ, rh-bFGF, rh-BMP2 and rx-BMP4 in an in vitro model. Orthopade 29:107–111PubMedGoogle Scholar
  5. 5.
    Reyes-Botella C, Vallecillo-Capilla MF, Ruiz C (2002) Effect of different growth factors on human cultured osteoblast-like cells. Cell Physiol Biochem 12:353–358CrossRefPubMedGoogle Scholar
  6. 6.
    Yanase T, Suzuki S, Goto K, Nombra M, Okabe T, Takayanagi R (2003) Aromatase in bone: roles of Vitamin D3 and androgens. J Steroid Biochem Mol Biol 86:393–397CrossRefPubMedGoogle Scholar
  7. 7.
    Reyes-Botella C, Montes MJ, Abadía-Molina AC, Vallecillo-Capilla MF, Ruiz C (1999) CD10 expression in cultured human osteoblast-like cells. Folia Biol (Praha) 45:257–260Google Scholar
  8. 8.
    Reyes-Botella C, Montes MJ, Vallecillo-Capilla MF, Olivares EG, Ruiz C (2000) Expression of molecules involved in antigen presentation and T cell activation by cultured human osteoblasts. J Periodontol 71:614–617CrossRefPubMedGoogle Scholar
  9. 9.
    Reyes-Botella C, Montes MJ, Vallecillo-Capilla MF, Olivares EG, Ruiz C (2002) Antigenic phenotype of cultured human osteoblast-like cells. Cell Physiol Biochem 12:359–364CrossRefPubMedGoogle Scholar
  10. 10.
    Saad B, Ciardelli G, Welti M, Uhlschmid GK, Neuenschwander P, Suter UW (1998) Degradable and highly porous polyesterurethane foam as biomaterial: effects and phagocytosis of degradation products in osteoblasts. J Biomed Mater Res 39:594–602CrossRefPubMedGoogle Scholar
  11. 11.
    Lohmann CH, Schwart Z, Köster G, Jahn U, Buchhorn GH, MacDougall MJ, Casasola D, Liu Y, Sylvia VL, Dean DD, Boyan BD (2000) Phagocytosis of wear debris by osteoblasts affects differentiation and local factor production in a manner dependent on particle composition. Biomaterial 21:551–561CrossRefGoogle Scholar
  12. 12.
    Vernes C, Roebuck KA, Chandrasekaran R, Dobai JG, Jacobs JJ, Glant TT (2000) Particulate wear debris activates protein tyrosine kinases and nuclear factor (B, which down-regulates type I collagen synthesis in human osteoblast. J Bone Miner Res 15:1756–1765CrossRefPubMedGoogle Scholar
  13. 13.
    Ruiz C, Pérez E, Vallecillo-Capilla MF, Reyes-Botella C (2003) Phagocytosis and allogeneic T cell stimulation by cultured human osteoblastic-like cells. Cell Physiol Biochem 13:309–314CrossRefPubMedGoogle Scholar
  14. 14.
    Nakamura H, Kenmotsu S, Sakai H, Ozawa H (1995) Localization of CD44, the hyaluronate receptor, on the plasma membrane of osteocytes and osteoclasts in rat tibiae. Cell Tissue Res 280:225–233PubMedGoogle Scholar
  15. 15.
    Groenewegen G, Buurman WA, Von Der Linden CJ (1985) Lymphokine dependence of in vivo expression of MHC class II antigens by endothelium. Nature 316:361–363CrossRefPubMedGoogle Scholar
  16. 16.
    Terai M, Kohno Y, Namba M, Unemiya T, Niwa K, Nakajima H, Mikata A (1990) Class II major histocompatibility antigen expression on coronary arterial endothelium in a patient with Kawasaki disease. Hum Pathol 21:231–234CrossRefPubMedGoogle Scholar
  17. 17.
    Majumdar MK, Keane-Moore M, Buyaner D, Hardy WB, Moorman MA, McIntosh KR, Mosca JD (2003) Characterization and functionality of cell surface molecules on human mesenchymal stem cells. J Biomed Sci 10:228–241PubMedCrossRefGoogle Scholar
  18. 18.
    Mucci A, Varesio L, Neglia R, Colombari B, Pastorino S, Blasi E (2003) Antifungal activity of macrophages engineered to produce IFNgamma: inducibility by picolinic acid. Med Microbiol Immunol (Berl) 192:71–78Google Scholar
  19. 19.
    Bost KL, Ramp WK, Nicholson NC, Bento JL, Marriott I, Hudson MC (1999) Staphylococcus aureus infection of mouse or human osteoblasts induced high levels of Interleukin-6 and Interleukin-12 production. J Infect Dis 180:1912–1920CrossRefPubMedGoogle Scholar
  20. 20.
    Trinchieri G, Gerosa F (1996) Immunoregulation by interleukin-12. J Leukoc Biol 59:505–511PubMedGoogle Scholar
  21. 21.
    O`Garra A (1998) Cytokines induce the development of functionally heterogeneous T helper cells subsets. Immunity 8:275–283CrossRefPubMedGoogle Scholar
  22. 22.
    Bachereau J, Briere F, Caux C, Davoust J, Lebeque S, Liu YJ, Pulendran B, Palucka K (2000) Immunobiology of dendritic cells. Ann Rev Immunol 18:767–811CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • O.  García-Martínez
    • 1
  • C. Reyes-Botella
    • 2
  • O. Aguilera-Castillo
    • 1
  • M.F. Vallecillo-Capilla
    • 2
  • C. Ruiz
    • 1
    • 3
    • 4
  1. 1.Department of Nursing, Physiology SectionUniversity of GranadaGranadaSpain
  2. 2.Department of StomatologyUniversity of GranadaGranadaSpain
  3. 3.Institute of NeurocienciasUniversity of GranadaGranadaSpain
  4. 4.Escuela Universitaria de Ciencias de la SaludGranadaSpain

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