Biologia Plantarum

, Volume 62, Issue 3, pp 451–461 | Cite as

Characterization and expression analysis of circadian clock genes in the diploid woodland strawberry Fragaria vesca

  • X. D. Chen
  • J. Wang
  • M. Z. Zhao
  • F. Zhao
Original paper


Strawberry is an economically important fruit crop worldwide. Circadian clock genes are endogenous timers that regulate a wide range of metabolic processes and consequently plant development. However, little is known about the circadian clock genes in strawberry. In the present work, we identified 12 primary circadian clock genes from the diploid woodland strawberry (Fragaria vesca L.) genome. Phylogenetic, conserved motif, and gene structure analyses revealed the evolutionary relationships of strawberry circadian clock genes with homologous genes from other species. Promoter analysis revealed different regulatory elements responding to abiotic and biotic stresses and phytohormones. We characterized the transcript patterns of strawberry circadian clock genes over a 48-h period. The expression patterns of seven circadian clock genes displayed circadian rhythms. We also examined the expression patterns of these genes in response to low-temperature stress and six of them showed an upregulated expression. Interestingly, most of these upregulated genes were highly expressed during the day. Our study reveals the characteristics of primary circadian clock components in diploid woodland strawberry and their responses to low-temperature stress and lays a foundation for future functional studies of these circadian clock genes during the growth and development of diploid woodland strawberry.

Additional key words

Arabidopsis thaliana cold stress gene expression phylogenetic analysis 



brother of lux arrhythmo


circadian clock-associated 1


CCA1 hiking expedition


early flowering


late elongated hypocotyl


lux arrhythmo


pseudo-response regulator


quantitative polymerase chain reaction




timing of CAB 1




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Supplementary material

10535_2018_793_MOESM1_ESM.pdf (1.6 mb)
Table 1 Suppl. Homologues of primary circadian clock genes in different plant species.


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Copyright information

© The Institute of Experimental Botany 2018

Authors and Affiliations

  1. 1.Institute of PomologyJiangsu Academy of Agricultural Sciences and Jiangsu Key Laboratory for Horticultural Crop Genetic ImprovementNanjingP.R. China

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