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BrEXL6, a GDSL lipase gene of Brassica rapa, functions in pollen development

  • Original paper
  • Published:
Biologia Plantarum

Abstract

Multiple allele-inherited male sterility has been widely used by breeders of Brassica rapa L. ssp. pekinensis, but the molecular mechanisms of male sterility are not yet clear. In this study, we isolated the full-length cDNA of a new gene (not included in the Brassica database). This gene, comprising 1 054 bp, encodes a 39.99 kDa protein with a Gly-Asp- Ser-Leu (GDSL)-lipase domain that is a member of the lipolytic protein GDSL family. The sequence of candidate gene is the most similar to extracellular lipase 6 (EXL6) of Arabidopsis and was therefore designated BrEXL6 and submitted to NCBI (accession No. JX131630.1). Reverse transcription semi-quantitative PCR and Western blot analysis showed that BrEXL6 and its encoded protein were significantly more expressed in fertile buds than in sterile buds. Quantitative PCR and in situ hybridization showed that BrEXL6 was highly expressed in the anthers of fertile buds, especially anthers at the pollen-development stages, but only weakly expressed in other tissues and floral organs of fertile plants and whole sterile plants. These results suggest that BrEXL6 is a pollen development-related gene. The results of this study provide clues for understanding the mechanisms underlying multiple allele-inherited male sterility.

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Abbreviations

CMS:

cytoplasmic male sterility

ESTs:

expressed sequence tags

EXL:

extracellular lipase 6

GDSL:

Gly-Asp- Ser-Leu

GMS:

genic male sterility

ORF:

open reading frame

RT-sqPCR:

reverse-transcription semi-quantitative PCR

SSH:

suppression subtractive hybridization

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Author notes

  1. X. Xin

    Present address: Agricultural Products Quality Supervision and Administration Bureau, Baoding, 071000, China

Authors and Affiliations

  1. Department of Horticulture, Shenyang Agricultural University, Shenyang, 110866, P.R. China

    R. Ji, H. Wang, X. Xin, S. Peng & H. Feng

  2. Department of Biology, Chungnam National University, Daejeon, 305764, South Korea

    Y. Hur

  3. Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Hohhot, 010031, P.R. China

    Z. Li

Authors
  1. R. Ji
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  2. H. Wang
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  3. X. Xin
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  4. S. Peng
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  5. Y. Hur
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  6. Z. Li
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  7. H. Feng
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Corresponding author

Correspondence to H. Feng.

Additional information

Acknowledgments: This work was supported by the National Natural Science Foundation of China [grant Nos. 31272157, 30800748] and the Liaoning Province Natural Science Foundation of China [grant No. 2013020071]. The first two authors contributed equally to this work.

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Ji, R., Wang, H., Xin, X. et al. BrEXL6, a GDSL lipase gene of Brassica rapa, functions in pollen development. Biol Plant 61, 685–692 (2017). https://doi.org/10.1007/s10535-017-0735-6

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  • DOI: https://doi.org/10.1007/s10535-017-0735-6

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