Cytochrome P450, CYP93A1, as defense marker in soybean
- 108 Downloads
Cytochrome P450, CYP93A1, is involved in the synthesis of the phytoalexin glyceollin in soybean (Glycine max L. Merr). The gene encoding CYP93A1 has been used as defense marker in soybean cell cultures, however, little is known regarding how this gene is expressed in the intact plant. To further understand the tissue-specific role of CYP93A1 in soybean defense, we analyzed the expression of this gene in mechanically damaged leaves and stems. In leaves, CYP93A1 was constitutively expressed; its expression did not change in response to mechanical damage. In stems, however, expression of CYP93A1 was induced as quickly as 4 h after mechanical damage and remained upregulated for at least 48 h. The induction of CYP93A1 was associated with the synthesis of glyceollins. In comparison to several other defense-related genes encoding cysteine protease inhibitors L1 and R1 and storage proteins vspA and vspB, CYP93A1 was the most strongly induced by stem wounding. The induction of CYP93A1 was observed only locally, not systemically. Similar stem expression patterns were consistently observed among three different soybean genotypes. The strong induction of CYP93A1 in mechanically damaged stems suggests an important role in the soybean stem defense response; therefore, this study expands the use of CYP93A1 as a defense response marker to stems, not just soybean cell cultures.
Additional key wordscysteine protease inhibitors glyceollin jasmonic acid mechanical damage storage proteins
cysteine proteinase inhibitor
translational elongation factor 1 subunit beta
vegetative storage protein
Unable to display preview. Download preview PDF.
- Botella, M.A., Xu, Y., Prabha, T.N., Zhao, Y., Narasimhan, M.L., Wilson, K.A., Nielsen, S.S., Bressan, R.A., Hasegawa, P.M.: Differential expression of soybean cysteine proteinase inhibitor genes during development and in response to wounding and methyl jasmonate. — Plant Physiol. 112: 1201–1210, 1996.CrossRefPubMedPubMedCentralGoogle Scholar
- Dafoe, N.J., Thomas, J.D., Shirk, P.D., Legaspi, M.E., Vaughan, M.M., Huffaker, A., Teal, P.E., Schmelz, E.A.: European corn borer (Ostrinia nubilalis) induced responses enhance susceptibility in maize. - PLoS ONE 8: 2013.Google Scholar
- Howe, G.A., Jander, G.: Plant immunity to insect herbivores. — Annu. Rev. Phytopathol. 59: 41–66, 2008.Google Scholar
- Neter, J., Wasserman, W., Kutner, M.H.: Applied Linear Regression Models. 2nd Ed. - Mcgraw-Hill, Irwin - Homewood 1989.Google Scholar
- Schmutz, J., Cannon, S.B., Schlueter, J., Ma, J., Mitros, T., Nelson, W., Hyten, D.L., Song, Q., Thelen, J.J., Cheng, J., Xu, D., Hellsten, U., May, G.D., Yu, Y., Sakurai, T., Umezawa, T., Bhattacharyya, M.K., Sandhu, D., Valliyodan, B., Lindquist, E., Peto, M., Grant, D., Shu, S., Goodstein, D., Barry, K., Futrell-Griggs, M., Abernathy, B., Du, J., Tian, Z., Zhu, L., Gill, N., Joshi, T., Libault, M., Sethuraman, A., Zhang, X.-C., Shinozaki, K., Nguyen, H.T., Wing, R.A., Cregan, P., Specht, J., Grimwood, J., Rokhsar, D., Stacey, G., Shoemaker, R.C., Jackson, S.A.: Genome sequence of the palaeopolyploid soybean. — Nature 463: 178–183, 2010.CrossRefPubMedGoogle Scholar
- Zavala, J.A., Casteel, C.L., Nabity, P.D., Berenbaum, M.R., DeLucia, E.H.: Role of cysteine proteinase inhibitors in preference of japanese beetles (Popillia japonica) for soybean (Glycine max) leaves of different ages and grown under elevated CO2. — Oecologia 161: 35–41, 2009.CrossRefPubMedGoogle Scholar