Characterization and expression analysis of histone deacetylases family RPD3/HDA1 in Populus trichocarpa
- 212 Downloads
Histone deacetylases (HDACs) catalyze histone deacetylation and play an important role in suppression of gene transcription in multiple cellular processes. HDACs are widely distributed in eukaryotes, however, detailed characterrization of HDACs in woody plants is not available. In this study, the sequences of reduced potassium dependency 3/histone deacetylase 1 (RPD3/HDA1) family proteins in black cottonwood (Populus trichocarpa Torr. & A. Gray) were characterized and their expression patterns in response to cold and salt stresses were determined. The RPD3/HDA1 proteins had conserved HDAC domains and can be divided into three classes based on sequence similarity and phylogenetic analysis. The transcripts of the HDAC genes were detected in different amounts in leaves, stems, and roots. The expressions of HDAC genes HDA902, HDA903, HDA904, HDA909, and HDA912 were up-regulated in a cold stress. Interestingly, in a salt stress, most of the HDAC genes were down-regulated. These results indicate that the poplar HDAC genes were regulated by the cold and salt stresses, and the members of the RPD3/HDA1 family play a role in stress responses.
Additional key wordsblack cottonwood cold gene expression salinity
histone deacetylase 1
molecular evolutionary genetic analysis
National Center for Biotechnology Information
open reading frame
reduced potassium dependency 3
polymerase chain reaction
Unable to display preview. Download preview PDF.
- Nicholas, K.B., Nicholas, H.B., Jr.: GeneDoc: a tool for editing and annotating multiple sequence alignments. - Software distributed by the authors, 1997.Google Scholar
- Pandey, R., Muller, A., Napoli, C.A., Selinger, D.A., Pikaard, C.S., Richards, E.J., Bender, J., Mount, D.W., Jorgensen, R.A.: Analysis of histone acetyltransferase and histone deacetylase families of Arabidopsis thaliana suggests functional diversification of chromatin modification among multicellular eukaryotes. — Nucl. Acids Res. 30: 5036–5055, 2002.CrossRefPubMedPubMedCentralGoogle Scholar
- Rossi, V., Locatelli, S., Varotto, S., Donn, G., Pirona, R., Henderson, D.A., Hartings, H., Motto, M.: Maize histone deacetylase hda101 is involved in plant development, gene transcription, and sequence-specific modulation of histone modification of genes and repeats. — Plant Cell 19: 1145–1162, 2007.CrossRefPubMedPubMedCentralGoogle Scholar
- Zhu, J., Jeong, J.C., Zhu, Y., Sokolchik, I., Miyazaki, S., Zhu, J.K., Hasegawa, P.M., Bohnert, H.J., Shi, H., Yun, D.J., Bressan, R.A.: Involvement of Arabidopsis HOS15 in histone deacetylation and cold tolerance. — Proc. nat. Acad. Sci. USA 105: 4945–4950, 2008.CrossRefPubMedPubMedCentralGoogle Scholar