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Exogenous sucrose can enhance tolerance of Arabidopsis thaliana seedlings to salt stress

  • Original Papers
  • Published:
Biologia Plantarum

Abstract

To investigate the physiological mechanisms of salt stress mitigated by exogenous sucrose, Arabidopsis thaliana seedlings grown on Murashige and Skoog medium were treated with 3 % (m/v) sucrose combined with 75, 150, and 225 mM NaCl for 3 d. Our results show that increased salinity significantly decreased the survival rate, fresh mass, content of proteins, chlorophyll a (Chl a), and chlorophyll b (Chl b), and activities of antioxidant enzymes, whereas enhanced the content of malondialdehyde. However, the treatment with sucrose significantly enhanced salt stress tolerance in the Arabidopsis seedlings by decreasing lipid peroxidation and increasing the activities of superoxide dismutase, peroxidase, and catalase, the content of proteins, Chl a, Chl b, anthocyanins, and the transcription of genes involved in anthocyanin biosynthesis. Thus, sucrose might reduce ROS-induced oxidative damage by enhancing activities of antioxidant enzymes and the content of anthocyanins, thereby preventing membrane peroxidation and denaturation of biomolecules.

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Abbreviations

APX:

ascorbate peroxidase

CAT:

catalase

Chl:

chlorophyll

DFR :

gene encoding dihydroflavonol 4-reductase

LDOX :

leucoanthocyanidin dioxygenase

MDA:

malondialdehyde

MS:

Murashige and Skoog

MYB :

gene encoding transcription factor

NBT:

nitroblue tetrazolium

POD:

peroxidase

ROS:

reactive oxygen species

SOD:

superoxide dismutase

TBA:

thiobarbituric acid

TT8 :

gene encoding transparent testa 8

References

  • Arnon, D.I.: Copper enzyme in isolated chloroplasts and polyphenoloxidase in Beta vulgaris. — Plant Physiol. 24: 1–15, 1949.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Bradford, M.M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. — Anal. Biochem. 72: 248–254, 1976.

    Article  PubMed  CAS  Google Scholar 

  • Cakmak I., Marschner H.: Magnesium deficiency and high light intensity on enhance activities of superoxide dismutase, peroxidase and glutathione reductase in bean leaves. — Plant Physiol. 98: 1222–1227, 1992.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Daiponmak, W., Theerakulpisut, P., Thanonkao, P., Vanavichit, A., Prathetha, P.: Changes of anthocyanin cyaniding-3-glucoside content and antioxidant activity in Thai rice varieties under salinity stress. — Science Asia 36: 286–291, 2010.

    Article  CAS  Google Scholar 

  • Das, P.K., Shin, D.H., Choi1, S.B., Park Y.: Sugar-hormone cross-talk in anthocyanin biosynthesis. — Mol. Cells 34: 501–507, 2010.

    Article  Google Scholar 

  • Flowers, T.J., Yeo, A.R.: Breeding for salinity resistance in crop plants: where next? — Aust. J. Plant Physiol. 22: 875–884, 1995.

    Article  Google Scholar 

  • Giannopolitis, C.N., Ries, S.K.: Superoxide dismutase. I. Occurrence in higher plants. — Plant Physiol. 59: 309–314, 1977.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Gould, K.S.: Nature’s Swiss army knife: the diverse protective roles of anthocyanins in leaves. — J. Biomed. Biotechnol. 5: 314–320, 2004.

    Article  Google Scholar 

  • Hossain, M.A., Kim, S., Kim, K.H., Lee, S.J., Lee H.: Flavonoid compounds are enriched in lemon balm (Melissa officinalis) leaves by a high level of sucrose and confer increased antioxidant activity. — Hortscience 44: 1907–1913, 2009.

    Google Scholar 

  • Lei, B., Huang, Y., Xie, J.J., Liu, Z.X., Zhen, A., Fan, M.L., Bie, Z.L.: Increased cucumber salt tolerance by grafting on pumpkin rootstock and after application of calcium. — Biol. Plant. 58: 179–184, 2014.

    Article  CAS  Google Scholar 

  • Li, J.T., Qiu, Z.B., Zhang, X.W., Wang L.S.: Exogenous hydrogen peroxide can enhance tolerance of wheat seedlings to salt stress. — Acta Physiol. Plant. 33: 835–842, 2011.

    Article  CAS  Google Scholar 

  • Loreti, E., Poggi, A., Novi, G., Alpi, A., Perata, P.: A genomewide analysis of the effects of sucrose on gene expression in Arabidopsis seedlings under anoxia. — Plant Physiol. 137: 1130–1138, 2005.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Meng, X., Yin, B., Feng, H.L., Zhang, S., Liang, X.Q., Meng, Q.W.: Overexpression of R2R3-MYB gene leads to accumulation of anthocyanin and enhanced resistance to chilling and oxidative stress. — Biol. Plant. 58: 121–130, 2014.

    Article  CAS  Google Scholar 

  • Miller, G., Suzuki, N., Ciftci-Yilmaz, S., Mittler, R.: Reactive oxygen species homeostasis and signaling during drought and salinity stresses. — Plant Cell Environ. 33: 453–467, 2010.

    Article  PubMed  CAS  Google Scholar 

  • Mittler, R.: Oxidative stress, antioxidants and stress tolerance. — Trends Plant Sci. 7: 405–410, 2002.

    Article  PubMed  CAS  Google Scholar 

  • Moustakas, M., Sperdouli, I., Kouna, T., Antonopoulou, C., Therios, I.: Exogenous proline induces soluble sugar accumulation and alleviates drought stress effects on photosystem II functioning of Arabidopsis thaliana leaves. — Plant Growth Regul. 65: 315–325, 2011.

    Article  CAS  Google Scholar 

  • Nakano, Y., Asada, K.: Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. — Plant Cell Physiol. 22: 867–880, 1981.

    CAS  Google Scholar 

  • Neill, S.O., Gould, K.S.: Anthocyanins in leaves: light attenuators or antioxidants ? — Funct. Plant Biol. 30: 865–873, 2003.

    Article  CAS  Google Scholar 

  • Oh, J.E., Kim, Y.H., Kim, J.H., Kwon, Y.R., Lee, H.: Enhanced level of anthocyanin leads to increased salt tolerance in Arabidopsis PAP1-D plants upon sucrose treatment. — J. korean Soc. appl. Biol. 1: 79–88, 2011.

    Google Scholar 

  • Ohto, M., Onai, K., Furukawa, Y., Aoki, E., Araki, T., Nakamura, K.: Effects of sugar on vegetative development and floral transition in Arabidopsis. — Plant Physiol. 127: 252–261, 2001.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Park, N.L., Xu, H., Li, X., Jang, I.H., Park, S.H., Ahn, G.H., Lim, Y.P., Kim, S.J., Park, S.U.: Anthocyanin accumulation and expression of anthocyanin biosynthetic genes in radish (Raphanus sativus). — J. Agr. Food Chem. 59: 6034–6039, 2011.

    Article  CAS  Google Scholar 

  • Predieri, S., Norman, H.A., Krizek, D.T.: Influence of UV-B radiation on membrane lipid composition and ethylene of evolution in ‘Doyenne d’Hiver’ pear shoots grown in vitro under different photosynthetic photon fluxes. — Environ. exp. Bot. 35: 151–160, 1995.

    Article  CAS  Google Scholar 

  • Qiu, Z.B., Li, Q., Bi, Z.Z., Yue, M.: Hydrogen peroxide acts as a signal molecule in CO2 laser pretreatment-induced osmotic tolerance in wheat seedlings. — Plant Soil Environ. 9: 403–408, 2011.

    Google Scholar 

  • Ramel, F., Sulmon, C., Cabello-Hurtado, F., Taconnat, L., Martin-Magniette, M.L., Renou, J.P., Amrani, A.E., Couée, I., Gouesbet, G.: Genome-wide interacting effects of sucrose and herbicide-mediated stress in Arabidopsis thaliana: novel insights into atrazine toxicity and sucroseinduced tolerance. — BMC Genom. 8: 450, 2007.

    Article  Google Scholar 

  • Ronchi, A., Farina, G., Gozzo, F., Tonelli, C.: Effects of a triazolic fungicide on maize plant metabolism: modifications of transcript abundance in resistance-related pathways. — Plant Sci. 130: 51–62, 1997.

    Article  CAS  Google Scholar 

  • Smeekens, S., Ma, J.K., Hanson, J., Rolland, F.: Sugar signals and molecular networks controlling plant growth. — Curr. Opin. Plant Biol. 13: 274–279, 2010.

    Article  PubMed  CAS  Google Scholar 

  • Solfanelli, C., Poggi, A., Loreti, E., Alpi, A., Perata, P.: Sucrose-specific induction of the anthocyanin biosynthetic pathway in Arabidopsis. — Plant Physiol. 140: 637–646, 2006.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Sperdouli, I., Moustakas, M.: Interaction of proline, sugars, and anthocyanins during photosynthetic acclimation of Arabidopsis thaliana to drought stress. — J. Plant Physiol. 169: 577–585, 2012.

    Article  PubMed  CAS  Google Scholar 

  • Sulmon, C., Gouesbet, G., Binet, F., Martin-Laurent, F., El Amrani, A., Couée, I.: Sucrose amendment enhances phytoaccumulation of the herbicide atrazine in Arabidopsis thaliana. — Environ. Pollut. 145: 507–515, 2007.

    Article  PubMed  CAS  Google Scholar 

  • Teng, S., Keurentjes, J., Bentsink, L., Koornneef, M., Smeekens, S.: Sucrose-specific induction of anthocyanin biosynthesis in Arabidopsis requires the MYB75/PAP1 gene. — Plant Physiol. 139: 1840–1852, 2005.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Wind, J., Smeekens, S., Hanson, J.: Sucrose: metabolite and signaling molecule. — Phytochemistry 71: 1610–1614, 2010.

    Article  PubMed  CAS  Google Scholar 

  • Xie, Z.X., Duan, L.S., Tian, X.L., Wang, B.M., Eneji, A.E., Li, Z.H.: Coronatine alleviates salinity stress in cotton by improving the antioxidative defense system and radicalscavenging activity. — J. Plant Physiol. 165: 375–384, 2008.

    Article  PubMed  CAS  Google Scholar 

  • Zhang, J.X., Kirham, M.B.: Drought stress-induced changes in activities of superoxide dismutase, catalase and peroxidase in wheat species. — Plant Cell Physiol. 35: 785–791, 1994.

    CAS  Google Scholar 

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Authors and Affiliations

  1. College of Life Science, Henan Normal University, Xinxiang, 453007, P.R. China

    Z. B. Qiu, Y. F. Wang, A. J. Zhu, F. L. Peng & L. S. Wang

Authors
  1. Z. B. Qiu
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  2. Y. F. Wang
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  3. A. J. Zhu
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  4. F. L. Peng
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  5. L. S. Wang
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Corresponding author

Correspondence to L. S. Wang.

Additional information

Acknowledgments: This research was supported by Project of Youth Back bone Teacher of Henan Province (2012GGJS-066) and the Henan Provincial Natural Science Foundation of China (142300410170). The first two authors equally contributed to this paper.

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Qiu, Z.B., Wang, Y.F., Zhu, A.J. et al. Exogenous sucrose can enhance tolerance of Arabidopsis thaliana seedlings to salt stress. Biol Plant 58, 611–617 (2014). https://doi.org/10.1007/s10535-014-0444-3

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  • DOI: https://doi.org/10.1007/s10535-014-0444-3

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