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Biologia Plantarum

, Volume 58, Issue 4, pp 611–617 | Cite as

Exogenous sucrose can enhance tolerance of Arabidopsis thaliana seedlings to salt stress

  • Z. B. Qiu
  • Y. F. Wang
  • A. J. Zhu
  • F. L. Peng
  • L. S. Wang
Original Papers

Abstract

To investigate the physiological mechanisms of salt stress mitigated by exogenous sucrose, Arabidopsis thaliana seedlings grown on Murashige and Skoog medium were treated with 3 % (m/v) sucrose combined with 75, 150, and 225 mM NaCl for 3 d. Our results show that increased salinity significantly decreased the survival rate, fresh mass, content of proteins, chlorophyll a (Chl a), and chlorophyll b (Chl b), and activities of antioxidant enzymes, whereas enhanced the content of malondialdehyde. However, the treatment with sucrose significantly enhanced salt stress tolerance in the Arabidopsis seedlings by decreasing lipid peroxidation and increasing the activities of superoxide dismutase, peroxidase, and catalase, the content of proteins, Chl a, Chl b, anthocyanins, and the transcription of genes involved in anthocyanin biosynthesis. Thus, sucrose might reduce ROS-induced oxidative damage by enhancing activities of antioxidant enzymes and the content of anthocyanins, thereby preventing membrane peroxidation and denaturation of biomolecules.

Additional key words

anthocyanins acorbate peroxidase chlorophyll catalase gene expression malondialdehyde NaCl peroxidase superoxide dismutase 

Abbreviations

APX

ascorbate peroxidase

CAT

catalase

Chl

chlorophyll

DFR

gene encoding dihydroflavonol 4-reductase

LDOX

leucoanthocyanidin dioxygenase

MDA

malondialdehyde

MS

Murashige and Skoog

MYB

gene encoding transcription factor

NBT

nitroblue tetrazolium

POD

peroxidase

ROS

reactive oxygen species

SOD

superoxide dismutase

TBA

thiobarbituric acid

TT8

gene encoding transparent testa 8

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Z. B. Qiu
    • 1
  • Y. F. Wang
    • 1
  • A. J. Zhu
    • 1
  • F. L. Peng
    • 1
  • L. S. Wang
    • 1
  1. 1.College of Life ScienceHenan Normal UniversityXinxiangP.R. China

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