Effect of endophyte infection on chlorophyll a fluorescence in salinity stressed rice
- 498 Downloads
We have earlier reported that the endophyte infection can enhance photosynthetic capacity and antioxidant enzyme activities in rice exposed to salinity stress. Now, the changes in primary photochemistry of photosystem (PS) II induced by Na2CO3 stress in endophyte-infected (E+) and endophyte-uninfected (E-) rice seedlings were studied using chlorophyll a fluorescence (OJIP-test). Performance indices (PIABS and PITotal) of E- and E+ rice seedlings revealed the inhibitory effects of Na2CO3 on PS II connectivity (occurrence of an L-band), oxygen evolving complex (occurrence of a K-band), and on the J step of the induction curves, associated with an inhibition of electron transport from plastoquinone A (QA) to plastoquinone B (QB). In E+ seedlings, Na2CO3 effects on L and K bands were much smaller, or even negligible, and also there was no pronounced effect on the J step. Furthermore, the OJIP parameters indicated that 20 mM Na2CO3 had a greater influence on the photosystem (PS) II electron transport chain than did the 10 mM Na2CO3, and that changes were greater in E- than in E+. Endophyte infection was therefore deemed to enhance the photosynthetic mechanism of Oryza sativa exposed to salinity stress.
Additional key wordselectron transport chain Na2CO3 OJIP test Oryza sativa photosystem
Unable to display preview. Download preview PDF.
- Hakim, M.A., Juraimi, A.S., Begum, M., Hanafi, M.M., Ismail, M.R., Selamat, A.: Effect of salt stress on germination and early seedling growth of rice (Oryza sativa L.). — Afr. J. Biotechnol. 9: 1911–1918, 29, 2010.Google Scholar
- Jedmowski, C., Ashoub, A., Bruggemann, W.: Reactions of Egyptian landraces of Hordeum vulgare and Sorghum bicolor to drought stress, evaluated by the OJIP fluorescence transient analysis. — Acta Physiol. Plant. 35: 345–354, 2013.Google Scholar
- Kane, K.H.: Effects of endophyte infection on drought stress tolerance of Lolium perenne accessions from the Mediterranean region. — Environ. exp. Bot. 71: 337–344, 2011.Google Scholar
- Strasser, R., Srivastava, A., Tsimilli-Michael, M.: The fluorescence transient as a tool to characterize and screen photosynthetic samples. — In: Yunus, M., Pathre, U., Mohanty, P. (ed.): Probing Photosynthesis: Mechanisms, Regulation and Adaptation. Pp. 445–483. Taylor & Francis Publishers, London 2000.Google Scholar
- Strasser, R.J., Tsimilli-Michael, M., Qiang, S., Goltsev, V.: Simultaneous in vivo recording of prompt and delayed fluorescence and 820-nm reflection changes during drying and after rehydration of the resurrection plant Haberlea rhodopensis. — Biochim. biophys. Acta 1797: 1313–1326, 2010.PubMedCrossRefGoogle Scholar
- Venkatesh, J., Upadhyaya, C.P., Yu, J.W., Hemavathi, A., Kim, D.H., Strasser, R.J., Park, S.W.: Chlorophyll a fluorescence transient analysis of transgenic potato overexpressing D-galacturonic acid reductase gene for salinity stress tolerance. — Hort. Environ. Biotechnol. 53: 320–328, 2012.CrossRefGoogle Scholar
- Wang, Z.F., Li, C.J., Jin, W.J., Nan, Z.B.: Effect of Neotyphodium endophyte infection on salt tolerance of Hordeum brevisubulatum (Trin.) Link. — Acta agr. sin. 17: 88–92, 2009.Google Scholar
- Yusuf, M.A., Kumar, D., Rajwanshi, R., Strasser, R.J., Tsimilli-Michael, M., Sarin, N.B.: Overexpression of γ-tocopherol methyl transferase gene in transgenic Brassica juncea plants alleviates abiotic stress: Physiological and chlorophyll a fluorescence measurements. — Biochim. biophys. Acta 1797: 1428–1438, 2010.PubMedCrossRefGoogle Scholar