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Biologia Plantarum

, Volume 58, Issue 1, pp 153–158 | Cite as

Production of triploid plants from endosperm cultures of Phlox drummondii

  • A. Razdan Tiku
  • M. K. Razdan
  • S. N. Raina
Brief Communication

Abstract

Triploid plants of ornamental Phlox drummondii Hook. were raised from cultures of endosperm excised from immature fruits having zygotic embryo at early dicotyledonous stage. Endosperm tissue was firstly cultured with the embryo on the Murashige and Skoog’s (MS) medium supplemented with 5 μM 6-benzylaminopurine (BAP) + 10 μM α-napthaleneacetic acid (NAA) for 7 d and recultured after the embryo was removed. A friable callus appeared two weeks after removal of the embryo and it became compact callus mass in another three weeks. Upon transfer of this 5-week-old callus to the MS medium with 10 μM BAP + 2.5 μM indole-3-acetic acid (IAA), maximum percentage of green nodular shoot buds appeared from which regenerated dwarf shoots. Elongation of the dwarf shoots, however, required transfer of the individual dwarf shoots excised from the callus on the fresh medium and best results achieved on medium with low concentration of IAA (0.5 μM) in presence of 10 μM BAP. The shoots were then rooted in vitro and plants subsequently established in pots containing soil. Over 70 % of plants were triploid with a chromosome number of 2n=3x=21. Size of stem, leaves, flowers, pollen, and stomata of these triploid plants were higher and the plants were more vigorous as compared to naturally occurring diploid plants. In particular, flowers showed bright colour with enlarged central eye adding to their ornamental value.

Additional key words

auxins callus cytokinins ex vitro transfer in vitro culture ploidy 

Abbreviations

BAP

6-benzylaminopurine

CH

casein hydrolysate

2,4-D

2,4-dichlorophenoxy acetic acid

IAA

indole-3-acetic acid

KIN

kinetin

NAA

α-napthaleneacetic acid

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  1. 1.Department of BotanyUniversity of DelhiDelhiIndia
  2. 2.Shyam Lal CollegeUniversity of DelhiShahdara, DelhiIndia

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