Biologia Plantarum

, Volume 57, Issue 3, pp 401–409 | Cite as

In vitro flowering red miniature rose

  • S. Zeng
  • S. Liang
  • Y. Y. Zhang
  • K. L. Wu
  • J. A. Teixeira da Silva
  • J. Duan


Using aseptic plantlets obtained from stem node explants of hybrid red miniature rose (Rosa hybrida cv. Fairy Dance), the effects of shoot physiological status, medium ingredients, and culture thermoperiod on in vitro flowering were evaluated. Shoot height, subculture media for shoot multiplication, sucrose concentration, plant growth regulators (PGRs), mineral substances in media, and thermoperiod had a significant effect on the percentage of in vitro flowering. Shoots 3 ± 0.2 or 2 ± 0.2 cm in height cultured on Murashige and Skoog (MS) medium containing 2.0 mg dm−3 6-benzyladenine (BA), 0.2 mg dm−3 α-naphthaleneacetic acid (NAA), and 20 g dm−3 sucrose were more suitable for in vitro flowering than shoots 4 ± 0.2, or 5 ± 0.2 cm in height. The most suitable sucrose concentration for in vitro flowering was 50 g dm−3 and the most suitable PGRs were a combination of 3.0 mg dm−3 BA and 0.1 mg dm−3 NAA. Increasing the potassium nitrate to ammonium nitrate ratio or increasing the phosphate concentration in MS medium had a positive effect on in vitro flowering. The percentage of in vitro flowering was significantly higher at day/night temperature of 28/20 °C than at other constant temperatures. The percentage of in vitro flowering shoots reached 68.33 % despite the occurrence of abnormal flowers and some unique developmental patterns. It makes miniature rose a potentially new in vitro experimental platform for research on the molecular mechanisms of flowering ornamental plants.

Additional key words

abnormal flower auxins cytokinins minerals Rosa hybrida thermoperiod 





Duncan’s multiple range test


indole-3-butyric acid




Murashige and Skoog medium


α-naphthaleneacetic acid


plant growth regulator


photosynthetic photon flux density






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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • S. Zeng
    • 1
  • S. Liang
    • 1
    • 3
  • Y. Y. Zhang
    • 1
    • 3
  • K. L. Wu
    • 1
  • J. A. Teixeira da Silva
    • 4
  • J. Duan
    • 1
    • 2
  1. 1.Key Laboratory of South China Agricultural Plant Genetics and BreedingSouth China Botanical Garden, Chinese Academy of SciencesGuangzhouChina
  2. 2.Key Laboratory of Plant Resources Conservation and Sustainable UtilizationSouth China Botanical Garden, Chinese Academy of SciencesGuangzhouChina
  3. 3.University of Chinese Academy of SciencesBeijingChina
  4. 4.Faculty of Agriculture and Graduate School of AgricultureKagawa UniversityMiki-cho, KagawaJapan

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