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Spatio-temporal distribution and methyl-esterification of pectic epitopes provide evidence of developmental regulation of pectins during somatic embryogenesis in Arabidopsis thaliana

  • Published:
Biologia Plantarum

Abstract

The aim of the present study was to describe the occurrence of three pectic epitopes, recognized by JIM7, LM19, and LM5 antibodies, during somatic (SE) and zygotic (ZE) embryogenesis in Arabidopsis thaliana. The epitopes recognized by JIM7 and LM19 antibodies showed different distributions during SE stages. Moreover, in the early stages of somatic embryo development, a cytoplasmic occurrence of LM19 epitope was detected. Distribution of a pectic epitope recognized by LM5 antibody corresponded to a vascular system differentiation pattern. Occurrence of LM5 epitope was the same in both zygotic and somatic embryos and often restricted to newly synthesized walls of two adjacent cells. These data suggest that both low and high methyl-esterified pectins (recognized by LM19 and JIM7 antibodies, respectively) are developmentally regulated during SE stages and (1→4)-β-D-galactan epitope (recognized by LM5 antibody) may play a role in cell cytokinesis.

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Abbreviations

HG:

homogalacturonan

IZE:

immature zygotic embryo

MS:

Murashige and Skoog

PEGs:

cuticular pegs

RG:

rhamnogalacturonan

SAM:

shoot apical meristem

SE:

somatic embryogenesis

ZE:

zygotic embryogenesis

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Authors and Affiliations

  1. Laboratory of Cell Biology, Faculty of Biology and Environmental Protection, University of Silesia, Jagiellońska 28, PL-40032, Katowice, Poland

    K. Sala, I. Potocka & E. Kurczynska

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  1. K. Sala
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  2. I. Potocka
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  3. E. Kurczynska
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Correspondence to K. Sala.

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Acknowledgements: This work was partly supported by grant N303 092 32/3176 from the Polish Ministry of Science and Higher Education. We thank Prof. Paul Knox (Centre for Plant Sciences, University of Leeds, UK) for the generous gift of antibodies.

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Sala, K., Potocka, I. & Kurczynska, E. Spatio-temporal distribution and methyl-esterification of pectic epitopes provide evidence of developmental regulation of pectins during somatic embryogenesis in Arabidopsis thaliana . Biol Plant 57, 410–416 (2013). https://doi.org/10.1007/s10535-013-0304-6

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  • DOI: https://doi.org/10.1007/s10535-013-0304-6

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