Biologia Plantarum

, Volume 57, Issue 3, pp 410–416 | Cite as

Spatio-temporal distribution and methyl-esterification of pectic epitopes provide evidence of developmental regulation of pectins during somatic embryogenesis in Arabidopsis thaliana

  • K. Sala
  • I. Potocka
  • E. Kurczynska


The aim of the present study was to describe the occurrence of three pectic epitopes, recognized by JIM7, LM19, and LM5 antibodies, during somatic (SE) and zygotic (ZE) embryogenesis in Arabidopsis thaliana. The epitopes recognized by JIM7 and LM19 antibodies showed different distributions during SE stages. Moreover, in the early stages of somatic embryo development, a cytoplasmic occurrence of LM19 epitope was detected. Distribution of a pectic epitope recognized by LM5 antibody corresponded to a vascular system differentiation pattern. Occurrence of LM5 epitope was the same in both zygotic and somatic embryos and often restricted to newly synthesized walls of two adjacent cells. These data suggest that both low and high methyl-esterified pectins (recognized by LM19 and JIM7 antibodies, respectively) are developmentally regulated during SE stages and (1→4)-β-D-galactan epitope (recognized by LM5 antibody) may play a role in cell cytokinesis.

Additional key words

galactan immunofluorescence microscopy JIM7 LM19 and LM5 antibodies pectin methyl-esterification 





immature zygotic embryo


Murashige and Skoog


cuticular pegs




shoot apical meristem


somatic embryogenesis


zygotic embryogenesis


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© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  1. 1.Laboratory of Cell Biology, Faculty of Biology and Environmental ProtectionUniversity of SilesiaKatowicePoland

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