Spatio-temporal distribution and methyl-esterification of pectic epitopes provide evidence of developmental regulation of pectins during somatic embryogenesis in Arabidopsis thaliana
The aim of the present study was to describe the occurrence of three pectic epitopes, recognized by JIM7, LM19, and LM5 antibodies, during somatic (SE) and zygotic (ZE) embryogenesis in Arabidopsis thaliana. The epitopes recognized by JIM7 and LM19 antibodies showed different distributions during SE stages. Moreover, in the early stages of somatic embryo development, a cytoplasmic occurrence of LM19 epitope was detected. Distribution of a pectic epitope recognized by LM5 antibody corresponded to a vascular system differentiation pattern. Occurrence of LM5 epitope was the same in both zygotic and somatic embryos and often restricted to newly synthesized walls of two adjacent cells. These data suggest that both low and high methyl-esterified pectins (recognized by LM19 and JIM7 antibodies, respectively) are developmentally regulated during SE stages and (1→4)-β-D-galactan epitope (recognized by LM5 antibody) may play a role in cell cytokinesis.
Additional key wordsgalactan immunofluorescence microscopy JIM7 LM19 and LM5 antibodies pectin methyl-esterification
immature zygotic embryo
Murashige and Skoog
shoot apical meristem
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- Bouton, S., Leboeuf, E., Mouille, G., Leydecker, M.-T., Talbotec, J., Granier, F., Lahaye, M., Höfte, H., Truong, H.-N.: QUASIMODO1 encodes a putative membrane-bound glycosyltransferase required for normal pectin synthesis and cell adhesion in Arabidopsis. — Plant Cell 14: 2577–2590, 2002.PubMedCrossRefGoogle Scholar
- Chapman, A., Blervacq, A.-S., Hendriks, T., Slomianny, C., Vasseur, J., Hilbert, J.-L.: Cell wall differentiation during early somatic embryogenesis in plants. II. Ultrastructural study and pectin immunolocalization on chicory embryos. — Can. J. Bot. 78: 824–831, 2000.Google Scholar
- Jarvis, M.C.: Structure and properties of pectin gels in plant cell walls. — Plant Cell Environ. 7: 153–164, 1984.Google Scholar
- Mohnen, D.: Biosynthesis of pectins and galactomannans. — In: Pinto, B.M. (ed.): Comprehensive Natural Products Chemistry. Vol. 3. Carbohydrates and Their Derivatives Including Tannins, Cellulose, and Related Lignins. Pp. 497–527. Elsevier, Oxford 1999.Google Scholar
- Ramirez, C., Chiancone, B. Testillano, P.S., Garcia-Fojeda, B., Germana, M.-A., Risueno, M.-C.: First embryogenic stages of Citrus microspore-derived embryos. — Acta Biol. cracov. Ser. Bot. 45: 53–58, 2003.Google Scholar
- Rose, J.K.C. (ed.): The Plant Cell Wall. — Blackwell, Oxford 2003.Google Scholar
- Satiat-Jeunemaitre, B., Hawes, C.: Immunocytochemistry for light microscopy. — In: Hawes, C., Satiat-Jeunemaitre, B. (ed.): Plant Cell Biology. A Practical Approach. Pp. 207–233. Oxford University Press, Oxford 2001.Google Scholar
- Siedlecka, A., Wiklund, S., Peronne, M.-A., Micheli, F., Leśniewska, J., Sethson, I., Edlund, U., Richard, L., Sundberg, B., Mellerowicz, E.J.: Pectin methyl esterase inhibits intrusive and symplastic cell growth in developing wood cells of Populus. — Plant Physiol. 146: 554–565, 2008.PubMedCrossRefGoogle Scholar
- Vitha, S., Baluska, F., Jasik, J., Volkmann, D., Barlow, P.W.: Steedman’s wax for F-actin visualization. — In: Staiger, C.J., Baluska, F., Volkmann, D., Barlow, P.W. (ed.): Actin: a Dynamic Framework for Multiple Plant Cell Function. Pp. 619–636. Kluwer Academic Publishers, Dordrecht 2000.CrossRefGoogle Scholar