Biologia Plantarum

, Volume 56, Issue 4, pp 775–779 | Cite as

The influence of the N- and C- terminal modifications of Potato virus X coat protein on virus properties

  • H. Hoffmeisterova
  • T. Moravec
  • H. Plchova
  • J. Folwarczna
  • N. Cerovska
Brief Communication


The Potato virus X (PVX)-based vector was used for the construction of N- and C-terminally modified PVX coat protein (XCP) chimeras. N-terminal XCP modifications do not influence the viral life cycle, whereas the simple XCP C-terminal fusion impedes the viral replication. We designed several C-terminally modified XCP chimeras and tested their viabilities in various Nicotiana benthamiana genotypes. Our results showed the negative impact of 3′-terminal modification of XCP on the chimera’s life cycle. To ensure chimeric constructs stability, the second copy of the last 60 nucleotides of XCP followed by the 3′-untranslated region (UTR) was added downstream of the recombinant sequence. Simultaneously, the first copy of the last 60 nucleotides of XCP was mutated in order to prevent recombination between the two identical sequences. The movement protein of Tobacco mosaic virus expressed in transgenic N. benthamiana plants positively affected the cell-to-cell spread of C-terminally modified XCP chimeras.

Additional key words

chimeric coat protein expression of recombinant protein Nicotiana benthamiana terminal fusion 



coat protein


Human papillomavirus type 16


epitope derived from the L2 CP of the HPV16 (aa 108–120)


N-terminally modified XCP


movement protein


Potato virus X




single-tailed particles


total soluble protein


untranslated region


virus-like particles


CP of the PVX

XCP-L2, XCP-L2-Δ, XCP-L2-mut

XCP with different C-terminal modifications


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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • H. Hoffmeisterova
    • 1
    • 2
  • T. Moravec
    • 1
  • H. Plchova
    • 1
  • J. Folwarczna
    • 1
    • 3
  • N. Cerovska
    • 1
  1. 1.Laboratory of Virology, Institute of Experimental BotanyAcademy of Sciences of the Czech RepublicPrague 6Czech Republic
  2. 2.Department of Crop ProtectionCzech University of Life Sciences PraguePrague 6Czech Republic
  3. 3.Department of Biochemistry, Faculty of ScienceCharles University in PraguePrague 2Czech Republic

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