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Biologia Plantarum

, Volume 56, Issue 2, pp 395–400 | Cite as

Genetic variability in regenerated plants of Ungernia victoris

  • O. M. Bublyk
  • I. O. Andreev
  • K. V. Spiridonova
  • V. A. Kunakh
Brief Communication

Abstract

To determine the suitability of micropropagation techniques developed for conserving rare medicinal herb Ungernia victoris we estimated the genetic fidelity of plants produced through direct regeneration from the bulb scale segments and organogenesis from long-term callus culture. Average value of the Jaccard’s distances between explant-derived regenerants and maternal plants calculated from RAPD data was 0.5 %, while that of estimated between callus-derived regenerants and maternal cell line was 4.2 %; average distances between the objects among the explant-derived and callus-derived regenerants were 0.7 % and 2.5 %, respectively. The data obtained suggest that conditions for in vitro culture applied in this work provide relatively high genetic stability of the species upon the direct regeneration in vitro and regeneration from the long-term cultured callus.

Additional key words

micropropagation RAPD-analysis somaclonal variation tissue culture 

Abbreviations

NAA

1-naphtalene acetic acid

PCR

polymerase chain reaction

RAPD

random amplified polymorphic DNA

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Notes

Acknowledgements

Authors would like to thank Volodymyr Adonin for the technical help in English manuscript preparation.

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • O. M. Bublyk
    • 1
  • I. O. Andreev
    • 1
  • K. V. Spiridonova
    • 1
  • V. A. Kunakh
    • 1
  1. 1.Institute of Molecular Biology and GeneticsNAS of UkraineKyivUkraine

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