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Biologia Plantarum

, Volume 56, Issue 3, pp 595–598 | Cite as

Imaging of early conifer embryogenic tissues with the environmental scanning electron microscope

  • V. Neděla
  • J. Hřib
  • B. Vooková
Brief Communication

Abstract

This article describes the usage of non-commercial environmental scanning electron microscope (ESEM) for the visualization of plant extracellular matrix in Abies alba and Abies numidica. Non sputter-coated samples free of using the common fixation technique observed at the relatively low humidity of the air environment with the pressure 550 Pa and the low temperature of the sample from −18 to −22°C give surprisingly very good results that show the natural structure of the tissues. This seems to be generally applicable. Moreover, a specially designed ionization detector of secondary electrons and a YAG:Ce3+ detector of backscattered electrons were used for better comparison.

Additional key words

Abies alba A. numidica extracellular matrix 

Abbreviations

SH medium

Schenk and Hildebrandt medium

AGPs

arabinogalactan proteins

ECM

extracellular matrix

ECMSN

extracellular matrix surface network

SEM

scanning electron microscope

ESEM

environmental SEM

SE

secondary electrons

BSE

back secondary electrons

YAG: Ce3+

yttrium aluminum garnet activated with trivalent cerium

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  1. 1.Institute of Scientific InstrumentsAcademy of Sciences of the Czech RepublicBrnoCzechoslovakia
  2. 2.Institute of Scientific InstrumentsAcademy of Sciences of the Czech RepublicBrnoCzechoslovakia
  3. 3.Czech Republic Institute of Plant Genetics and BiotechnologySASNitraSlovak Republic

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