Comparison of antioxidant responses to cadmium and lead in Bruguiera gymnorrhiza seedlings
- 163 Downloads
Seedlings of mangrove plant Bruguiera gymnorrhiza cultured in sand with Hoagland’s nutrient solution were treated with 1 to 30 mM Cd(NO3)2 or Pb(NO3)2 for 2 months. In all Cd/Pb treatments, the malondialdehyde content increased while the chlorophyll content declined. Peroxidase (POD) and superoxide dismutase (SOD) activities in roots increased at moderate Cd/Pb concentrations (1–10 mM), whereas decreased at higher concentrations (20–30 mM). Catalase (CAT) activity in roots was inhibited by 1–10 mM Cd but enhanced by 1–10 mM Pb. The activities of POD, SOD and CAT in leaves were less affected by Cd and Pb than in roots. A new SOD and three CAT isoenzymes were induced by Pb. In contrast, no additional SOD and CAT isoenzymes were induced by Cd.
Additional key wordsantioxidant enzymes catalase mangrove peroxidase superoxide dismutase
polyacrylamide gel electrophoresis
reactive oxygen species
Unable to display preview. Download preview PDF.
This work was carried out with financial support from the Natural Science Foundation of Guangxi Province (GuiKeZi No. 0991022Z).
- Ding, B.L., Zhang, Z.L.: The Experimental Guide for Modern Plant Physiology. — Science Press, Beijing 1999.Google Scholar
- Wang, Y.D.: The Experimental Guide for Plant Science. — Higher Education Press, Beijing 2001.Google Scholar
- Zhang, J., Cui, S., Li, J., Kirkham, M.B.: Protoplasmic factors, antioxidant responses, and chilling resistance in maize. — Plant Physiol. Biochem. 33: 567–575, 1995.Google Scholar