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Transcriptome analysis of an invasive weed Mikania micrantha

Abstract

As an initial step towards understanding the molecular mechanisms by which plants become invasive, we present here the first transcriptome analysis for an invasive weed Mikania micrantha. The analysis was based on the 75-nucleotide short reads data generated by the Illumina Genome Analyzer II system. A total of 31 131 unique sequences were assembled de novo based on 8.7 million filtered paired-end sequence reads for the transcriptome of an individual M. micrantha growing in the field. 73 % of the unique sequences showed significant similarity to existing proteins in the NCBI database, and 21 448 could be grouped based on gene ontology assignments. Of particular interest are the sequences that share homology with genes involved in genome evolution, plasticity, secondary metabolism and defense responses.

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Abbreviations

EST:

extensive expressed sequence tag

PCR:

polymerase chain reaction

GO:

Gene ontology

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Acknowledgments

The work was supported by National Natural Science Foundation of China (40876075, 31070290, 30730008 and 40976081), National Basic Research Program of China (2007CB815701, 2009CB119204), the Natural Science Foundation of Guangdong Province (8151027501000089), Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry, the Fundamental Research Funds for the Central Universities (09lgpy35), SKLBC09A06 and SKLBC09F06.

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Correspondence to Y. -L. Huang or S. -H. Shi.

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Huang, Y.L., Fang, X.T., Lu, L. et al. Transcriptome analysis of an invasive weed Mikania micrantha . Biol Plant 56, 111–116 (2012). https://doi.org/10.1007/s10535-012-0024-3

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Additional key words

  • gene ontology
  • Illumina Genome Analyzer II system
  • sequence annotation