Biologia Plantarum

, Volume 55, Issue 3, pp 572–576 | Cite as

Selection of co-transformed Dendrobium Sonia 17 using hygromycin and green fluorescent protein

  • C. S. Tee
  • M. Maziah
  • C. S. Tan
  • M. P. Abdullah
Brief Communication


Dendrobium Sonia 17 calluses were used for co-transformation study using particle bombardment. The bombarded transformed callus tissues were selected using half-strength MS medium containing 25 mg dm−3 hygromycin. Expression of green fluorescent protein (GFP) was observed in the callus and protocorm-like body (PLBs) tissues survived on the selection medium. The presence of green fluorescence protein (sgfp), hygromycin-B-phosphotransferase (hptII) and β-glucuronidase (uidA) genes in the transformed tissues were verified using PCR, Southern blot and dot blot analyses. Based on the results from PCR and expression of sgfp and uidA genes in the calluses and PLBs survived from hygromycin selection, we reported the co-transformation of sgfp, hptII and uidA genes into Dendrobium Sonia 17. GFP-expressing tissues were also observed in the regenerated transformed plantlets.

Additional key words

bombardment β-glucuronidase PCR phosphotransferase Southern blot 



green fluorescent protein




Murashige and Skoog


protocorm-like bodies


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The authors wish to thank Dr. J. Sheen (Boston, USA) for kindly providing the GFP constructs and Dr. S. Manickam (Universiti Putra Malaysia) for the pSMDFR plasmid. This research was supported by Malaysia government’s IRPA MOSTE- TOP-DOWN project # 01-02-04-T001.


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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • C. S. Tee
    • 1
    • 3
  • M. Maziah
    • 1
  • C. S. Tan
    • 2
  • M. P. Abdullah
    • 1
  1. 1.Department of Biochemistry, Faculty of Biotechnology and Biomolecular SciencesUniversiti Putra MalaysiaSerdang, SelangorMalaysia
  2. 2.Malaysian Agricultural Research and Development InstituteSerdang, SelangorMalaysia
  3. 3.Department of Biological Science, Faculty of ScienceUniversiti Tunku Abdul RahmanKampar, PerakMalaysia

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