Effects of leaf soluble sugars content and net photosynthetic rate of quince donor shoots on subsequent morphogenesis in leaf explants
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The effects of different growth conditions (ventilated and closed vessels, medium with 0, 15 and 30 g dm−3 sucrose) during proliferation of donor quince (Cydonia oblonga Mill.) shoots (stage I) on net photosynthetic rate and soluble sugars content were evaluated. In order to assess the influence of these physiological parameters on morphogenesis, leaf explants harvested from donor shoots were induced to form somatic embryos and adventitious roots under ventilated and closed Petri dishes (stage II). Natural ventilation and low sucrose contents (0–15 g dm−3) promoted the photosynthetic rate of quince shoots whereas biomass accumulation was the highest in those shoots cultured with 30 g dm−3 sucrose in both vessel types and 15 g dm−3 sucrose under natural ventilation. Increasing sucrose content in the medium induced greater accumulation of sucrose in leaf tissues of donor shoots. The content of reducing sugars was higher than that of sucrose, and it appeared to be higher in shoots cultured under natural ventilation compared to those in closed vessels. Somatic embryogenesis and root regeneration were influenced by stage I and II treatments. A significant correlation between sucrose content in the leaves of donor shoots and the number of somatic embryos regenerated was found, suggesting that identification of biochemical and physiological characteristics of donor shoots associated with increased regeneration ability might be helpful for improving morphogenesis in plant tissue culture.
Additional key wordsadventitious roots Cydonia oblonga reducing sugars somatic embryos sucrose ventilated vessels
net photosynthetic rate
photon flux density
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We would like to thank Dr. A. Pardossi for advices on soluble sugars analysis and F. Rocco and G. Fiaschi for excellent technical assistance.
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