Effects of leaf soluble sugars content and net photosynthetic rate of quince donor shoots on subsequent morphogenesis in leaf explants
- 151 Downloads
The effects of different growth conditions (ventilated and closed vessels, medium with 0, 15 and 30 g dm−3 sucrose) during proliferation of donor quince (Cydonia oblonga Mill.) shoots (stage I) on net photosynthetic rate and soluble sugars content were evaluated. In order to assess the influence of these physiological parameters on morphogenesis, leaf explants harvested from donor shoots were induced to form somatic embryos and adventitious roots under ventilated and closed Petri dishes (stage II). Natural ventilation and low sucrose contents (0–15 g dm−3) promoted the photosynthetic rate of quince shoots whereas biomass accumulation was the highest in those shoots cultured with 30 g dm−3 sucrose in both vessel types and 15 g dm−3 sucrose under natural ventilation. Increasing sucrose content in the medium induced greater accumulation of sucrose in leaf tissues of donor shoots. The content of reducing sugars was higher than that of sucrose, and it appeared to be higher in shoots cultured under natural ventilation compared to those in closed vessels. Somatic embryogenesis and root regeneration were influenced by stage I and II treatments. A significant correlation between sucrose content in the leaves of donor shoots and the number of somatic embryos regenerated was found, suggesting that identification of biochemical and physiological characteristics of donor shoots associated with increased regeneration ability might be helpful for improving morphogenesis in plant tissue culture.
Additional key wordsadventitious roots Cydonia oblonga reducing sugars somatic embryos sucrose ventilated vessels
net photosynthetic rate
photon flux density
Unable to display preview. Download preview PDF.
We would like to thank Dr. A. Pardossi for advices on soluble sugars analysis and F. Rocco and G. Fiaschi for excellent technical assistance.
- Ahkami, A.H., Lischewski, S., Haensch, K.-T., Porfirova, S., Hofmann, J., Rolletschek, H., Melzer, M., Franken, P., Hause, B., Druege, U., Hajirezaei, M.R.: Molecular physiology of adventitious root formation in Petunia hybrida cuttings: involvement of wound response and primary metabolism. — New Phytol. 181: 613–625, 2008.PubMedCrossRefGoogle Scholar
- Brown, D.C.W., Thorpe, T.A.: Plant regeneration by organogenesis. — In: Vasil, I. K. (ed.): Cell Culture and Somatic Cell Genetics of Plants. Vol. 3: Plant Regeneration and Genetic Variability. Pp. 49–65. Academic Press, Orlando 1986.Google Scholar
- Desjardins, Y.: Carbon nutrition in vitro — regulation and manipulation of carbon assimilation in micropropagated system. — In: Aitken-Christie, J., Kozai, T., Smith, M.A.L. (ed.) Automation and Environmental Control in Plant Tissue Culture. Pp 441–471. Kluwer Academic Publishers, Dordrecht 1995.Google Scholar
- Fisichella, M., Morini, S.: Somatic embryo and root regeneration from quince leaves cultured in ventilated vessels or under different oxygen and carbon dioxide levels. — In Vitro cell. dev. Biol. Plant 39: 402–408, 2003.Google Scholar
- Fujiwara, K., Kozai, T., Watanabe, I.: Fundamental studies on environments in plant tissue culture. (3) Mesasurements of carbon dioxide gas concentration in closed vessels containing tissue cultured plantlets and estimates of net photosynthetic rates of plantlets. — J. agr. Metereol. 43: 21–30, 1987.Google Scholar
- George, E.F., Sherrington, P.D. (ed.): Plant Propagation by Tissue Culture. — Eastern Press, Reading 1984.Google Scholar
- Hawk, P.B., Bergham, O. (ed.): Practical Physiological Chemistry. 7th Edition. — Blakiston, Philadelphia 1943.Google Scholar
- Kozai, T.: Photoautotrophic micropropagation. — In Vitro cell. dev. Biol. Plant 27: 47–51, 1991.Google Scholar
- Kozai, T., Fujiwara, K., Watanabe, I.: Fundamental studies of environments in plant tissue culture vessels. (2) Effects of stoppers and vessels on gas exchange rates between inside and outside of vessels closed with stoppers. — J. agr. Metereol. 42: 119–127, 1986.Google Scholar
- Kozai, T., Iwabuchi, K., Watanabe, K., Watanabe, I.: Photoautotrophic and photomixotrophic growth of strawberry plantlets in vitro and changes in nutrient composition of the medium. — Plant Cell Tissue Organ Cult. 25: 107–115, 1991.Google Scholar
- Kubota, C., Ezawa, M., Kozai, T., Wilson, S.B.: In situ estimation of carbon balance of in vitro sweetpotato and tomato plantlets cultured with varying initial sucrose concentrations in the medium. — J. amer. Soc. hort. Sci. 127: 963–970, 2002.Google Scholar
- Li, M., Leung, D.W.M.: Starch accumulation is associated with adventitious root formation in hypocotyl cuttings of Pinus radiata. — J. Plant Growth Regul. 19: 423–428, 2000.Google Scholar
- Martin, A.B., Cuadrado, Y., Guerra, H., Gallego, P., Hita, O., Martin, L., Dorado, A., Villalobos, N.: Differences in the contents of total sugars, reducing sugars, starch and sucrose in embryogenic and non-embryogenic calli from Medicago arborea L. — Plant Sci. 154: 143–151, 2000.PubMedCrossRefGoogle Scholar
- Nhut, D.T., Huong, N.T.D., Van Le, B., Da Silva, J.T., Fukai, S., Tanaka, M.: The changes in shoot regeneration potential of protocorm-like bodies derived from Lilium longiflorum young stem explants exposed to medium volume, pH, light intensity and sucrose concentration pretreatment. — J. hort. Sci. Biotechnol. 77: 79–82, 2002.Google Scholar
- Nieves, N., Segura-Nieto, M., Blanco, M.A., Sanchez, M., Gonzalez, A., Gonzalez, J.L., Castillo, R.: Biochemical characterization of embryogenic and non-embryogenic calluses of sugarcane. — In Vitro cell. dev. Biol. Plant 39: 343–345, 2003.Google Scholar
- Zimmermann, M.H., Ziegler, H.: List of sugar alcohols in sievetube exudates. — In: Zimmermann, M.H., Milburn, J.A. (ed.) Encyclopedia of Plant Physiology. New Series. Pp. 480–503. Springer-Verlag, Berlin 1975.Google Scholar