Biologia Plantarum

, Volume 55, Issue 1, pp 169–172 | Cite as

In vitro regeneration of an endangered medicinal plant Picrorhiza scrophulariiflora

  • P. Bantawa
  • O. Saha-Roy
  • S. Kumar Ghosh
  • T. Kumar Mondal
Brief Communication


A reproducible in vitro regeneration system for Nepalese kutki (Picrorhiza scrophulariiflora Pennell) was developed from in vitro leaf derived callus. Induction of more than seven shoot buds per explant was achieved on Woody plant medium (WPM) supplemented with 0.53 μM α-napthaleneacetic acid (NAA) and 0.23 μM kinetin (KIN). The shoots were elongated on WPM supplemented with 0.44 μM 6-benzylaminopurine (BAP) and rooted on WPM supplemented with 5.3 μM NAA within 2 weeks. The random amplified polymorphic DNA (RAPD) analysis indicated genetic uniformity of the micropropagated plants with its donor plants.

Additional key words

auxins callus induction cytokinins picroside 





2,4-dichlorophenoxyacetic acid




α-napthaleneacetic acid


Woody plant medium


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The authors are thankful to Department of Biotechnology and Department of Science and Technology, Government of India for financial assistance, Mr. Bijoy Gurung for his help to conduct the survey and Mr. Kamal Das for his assistance.


  1. Alagumanian, S., Perumal, V.S., Balachandra, R., Rameshkannan, K., Rao, M.V.: Plant regeneration from leaf and stem explants of Solanum tribolatum L. — Curr. Sci. 86: 1478–1480, 2004.Google Scholar
  2. Bantawa, P.: Identification of chemically superior line and analysis of genetic diversity by PCR based markers of Picrorhiza and Gaultheria, two most endangered medicinal plants of Eastern Himalayas. — Ph.D. Thesis, Kalyani University, Kalyani 2009.Google Scholar
  3. Bantawa, P., Ghosh, S.K., Bhandari, P., Singh, B., Ghosh, P.D., Ahuja, P.S., Mondal, T.K.: Micropropagation of an elite line of Picrorhiza scrophulariiflora, Pennell, an endangered high valued medicinal plant of the Indo-China Himalayan region. — Med. aromat. Plant Sci. Biotechnol. (in press), 2010.Google Scholar
  4. Bantawa, P., Maitra, S., Ghosh, S.K., Ghosh, P.D., Mondal, T.K.: Status and conservation threats of Picrorhiza scrophulariiflora Pennell. (Scrophulariaceae): an endangered high valued medicinal plant of Indo-China Himalayan region. — Bioremediation, Biodiversity, Bioavailability 3: 15–22, 2009a.Google Scholar
  5. Bantawa, P., Saha-Roy, O., Ghosh, P.D., Mondal, T.K.: Effect of bavistin and adenine sulphate on shoot multiplication of Picrorhiza scrophulariiflora Pennell.: an endangered medicinal plant of Indo-China Himalayan regions. — Plant Tissue Cult. Biotechnol. 19: 237–245, 2009b.Google Scholar
  6. Das, M., Pal, A.: Clonal propagation and production of genetically uniform regenerants from axillary meristems of adult bamboo. — J. Plant Biochem. Biotechnol. 14: 185–188, 2005.Google Scholar
  7. Chandra, B., Palni, L.M.S., Nandi, S.K.: Propagation and conservation of Picrorhiza Kurroa Royle ex Benth: an endangered Himalayan medicinal herb of high commercial value. — Biodiversity Conservation 15: 2325–2338, 2006.CrossRefGoogle Scholar
  8. Hara, H., Chater, A.O., Williams, L.H.J.: An Enumeration of the Flowering Plants of Nepal. — Trustees of British Museum, London 1982.Google Scholar
  9. Huang, W.J., Ning, G.G., Liu, G.F., Bao, M.Z.: Determination of genetic stability of long-term micropropagated plantlets of Platanus acerifolia using ISSR markers. — Biol. Plant. 53: 159–163, 2009.CrossRefGoogle Scholar
  10. Jia, Q., Hong, M.F., Minter, D.: Pikuroside: a novel iridoid from Picrorhiza kurroa. — J. natur. Prod. 62: 901–903, 1999.CrossRefGoogle Scholar
  11. Lal, N., Ahuja, P.S., Kukreja, A.K., Pandey, B.: Clonal propagation of Picrorhiza kurroa Royle ex Benth. by shoot tip culture. — Plant Cell Rep. 7: 202–205, 1988.CrossRefGoogle Scholar
  12. Li, J.X., Li, P., Tezunka, Y., Namba, T., Kadota, S.: Three phenylethanoid glycosides and an iridoid glycoside from Picrorhiza scrophulariiflora. — Phytochemistry 48: 537–542, 1998.CrossRefPubMedGoogle Scholar
  13. Lloyd, G., McCown, W.: Commercially feasible micropropagation of mountain laurel Kalmia latifolia by use of shoot tips culture. — Combined Proc. int. Plant Soc. 30: 421–427, 1980.Google Scholar
  14. Mondal, T.K., Chand, P.K.: Detection of genetic instability among the miocropropagated tea (Camellia sinensis) plants. — In vitro cell. dev. Biol. Plant 37: 1–5, 2002.Google Scholar
  15. Naika, H.R., Krishna, V.: Plant regeneration from callus culture of Clenatis gouriana Roxb. — a rare medicinal plant. — Turk. J. Biol. 32: 99–103, 2008.Google Scholar
  16. Pandey, A., Bag, N., Chandra, B., Palni, L.M.S.: Biological hardening: a promising technology for tissue culture industry. — In: Nandi, S.K., Plani, L.M.S, Kumar, A. (ed.): Role of Plant Tissue Culture in Biodiversity Conservation and Economic Development. Pp. 565–577. Gyanodaya Prakashan, Nainital 2002.Google Scholar
  17. Saha, S., Mukhopadhyay, M.J., Mukhopadhyay, S.: In vitro clonal propagation through bud culture of Hemidesmus indicus (L) R. Br: an important medicinal herb. — J. Plant Biochem. Biotechnol. 12: 61–64, 2003.Google Scholar
  18. Sivakumar, G., Krishnamurthy, K.V.: Micropropagation of Gloriosa superba L., an endangered species of Asia and Africa. — Curr. Sci. 78: 30–32, 2000.Google Scholar
  19. Smith, H.F., Kroes, B.H., Van den Berg, A.J.J., Van der Wal, D., Van den Worm, E., Beukelman, C.J., Van Dijk, H., Labadie, R.P.: Immunomodulatory and anti-inflammatory activity of Picrorhiza scrophulariiflora, Pennell. — J. Ethnopharmacol. 73: 101–109, 2000.CrossRefGoogle Scholar
  20. Sokal, R., Rohlf, FJ.: Introduction to Biostatistics. 2nd Ed. — Freeman WH, New York 1987.Google Scholar
  21. Upadhyay, R., Arumugam, N., Bhojwani, S.S.: In vitro propagation of Picrorhiza kurroa Royle ex Benth. — an endangered species of medicinal importance. — Phytomorphology 39: 235–242, 1989.Google Scholar

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • P. Bantawa
    • 1
  • O. Saha-Roy
    • 1
  • S. Kumar Ghosh
    • 1
  • T. Kumar Mondal
    • 1
  1. 1.Biotechnology Laboratory, Faculty of HorticultureUttar Banga Krishi ViswavidalayaPundibari, Cooch BeharIndia

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