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Biologia Plantarum

, Volume 55, Issue 1, pp 169–172 | Cite as

In vitro regeneration of an endangered medicinal plant Picrorhiza scrophulariiflora

  • P. Bantawa
  • O. Saha-Roy
  • S. Kumar Ghosh
  • T. Kumar Mondal
Brief Communication

Abstract

A reproducible in vitro regeneration system for Nepalese kutki (Picrorhiza scrophulariiflora Pennell) was developed from in vitro leaf derived callus. Induction of more than seven shoot buds per explant was achieved on Woody plant medium (WPM) supplemented with 0.53 μM α-napthaleneacetic acid (NAA) and 0.23 μM kinetin (KIN). The shoots were elongated on WPM supplemented with 0.44 μM 6-benzylaminopurine (BAP) and rooted on WPM supplemented with 5.3 μM NAA within 2 weeks. The random amplified polymorphic DNA (RAPD) analysis indicated genetic uniformity of the micropropagated plants with its donor plants.

Additional key words

auxins callus induction cytokinins picroside 

Abbreviations

BAP

6-benzylaminopurine

2,4-D

2,4-dichlorophenoxyacetic acid

KIN

kinetin

NAA

α-napthaleneacetic acid

WPM

Woody plant medium

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Notes

Acknowledgments

The authors are thankful to Department of Biotechnology and Department of Science and Technology, Government of India for financial assistance, Mr. Bijoy Gurung for his help to conduct the survey and Mr. Kamal Das for his assistance.

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • P. Bantawa
    • 1
  • O. Saha-Roy
    • 1
  • S. Kumar Ghosh
    • 1
  • T. Kumar Mondal
    • 1
  1. 1.Biotechnology Laboratory, Faculty of HorticultureUttar Banga Krishi ViswavidalayaPundibari, Cooch BeharIndia

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