In vitro regeneration of an endangered medicinal plant Picrorhiza scrophulariiflora
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A reproducible in vitro regeneration system for Nepalese kutki (Picrorhiza scrophulariiflora Pennell) was developed from in vitro leaf derived callus. Induction of more than seven shoot buds per explant was achieved on Woody plant medium (WPM) supplemented with 0.53 μM α-napthaleneacetic acid (NAA) and 0.23 μM kinetin (KIN). The shoots were elongated on WPM supplemented with 0.44 μM 6-benzylaminopurine (BAP) and rooted on WPM supplemented with 5.3 μM NAA within 2 weeks. The random amplified polymorphic DNA (RAPD) analysis indicated genetic uniformity of the micropropagated plants with its donor plants.
Additional key wordsauxins callus induction cytokinins picroside
Woody plant medium
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The authors are thankful to Department of Biotechnology and Department of Science and Technology, Government of India for financial assistance, Mr. Bijoy Gurung for his help to conduct the survey and Mr. Kamal Das for his assistance.
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