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Biologia Plantarum

, Volume 55, Issue 1, pp 27–34 | Cite as

Silencing of the LeSGR1 gene in tomato inhibits chlorophyll degradation and exhibits a stay-green phenotype

  • Z. -L. Hu
  • L. Deng
  • B. Yan
  • Y. Pan
  • M. Luo
  • X. -Q. Chen
  • T. -Z. Hu
  • G. -P. Chen
Article

Abstract

The full-length cDNA of LeSGR1 was cloned from tomato by RT-PCR and RACE. The cDNA encoded a protein of 272 amino acid residues and was deposited in GenBank (accession No. DQ100158). Northern analysis suggests that LeSGR1 gene specifically expresses in senescent leaves and mature fruits of tomatoes. Desiccation and flooding induce the expression of LeSGR1 in tomato leaves and stems. Both in ethylene-insensitive mutants (Nr) and ripening inhibitor mutants (rin), the expression of LeSGR1 is markedly decreased compared with that in the wild type. Alignment of the nucleotide sequence of SGR1 cloned from the tomato green flesh (gf) mutant with that from the wild type tomato shows a single nucleotide change leading to an amino acid substitution in gf mutant. Furthermore, LeSGR1 gene silencing by RNA interference results in inhibited chlorophyll degradation similar to the phenotype in gf mutant. Thus, we conclude that LeSGR1 is crucial to chlorophyll degradation and the mutation of SGR1 protein might be responsible for gf tomato properties.

Additional key words

desiccation flooding Lycopersicon esculentum mutants senescence 

Abbreviations

AC++

Ailsa Craig

B

breaker

Chl

chlorophyll

dpa

days post anthesis

gf

green flesh

IMG

immature green

MG

mature green

ML

mature leaf

Nr

never ripe

ORF

open reading frame

RACE

rapid amplification of cDNA ends

rin

ripening inhibitor

RT PCR

reverse transcriptase polymerase chain reaction

SL

senescent leaf

UTR

untranslated region

YL

young leaf

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Notes

Acknowledgments

This work was supported by the National Natural Science Foundation of China (No.30771464 and No.30871709.) and by China Postdoctoral Science Foundation (No.20060390677 and No.20070420717). We thank Prof. J.R. Botella (University of Queensland) for critical reading of the manuscript.

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Z. -L. Hu
    • 1
  • L. Deng
    • 1
  • B. Yan
    • 1
  • Y. Pan
    • 1
  • M. Luo
    • 1
  • X. -Q. Chen
    • 2
  • T. -Z. Hu
    • 1
  • G. -P. Chen
    • 1
  1. 1.Key Laboratory of Biorheological Science and Technology, Ministry of Education, Bioengineering CollegeChongqing UniversityChongqingP.R. China
  2. 2.Beijing Agro-Biotechnology Research CenterBeijingP.R. China

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