Biologia Plantarum

, Volume 54, Issue 4, pp 753–756 | Cite as

The effect of 5-azacytidine on wheat seedlings responses to NaCl stress

  • L. Zhong
  • Y. H. Xu
  • J. B. Wang
Brief Communication


The effect of 5-azacytidine (5-azaC) on the alleviation of damaging effects of NaCl treatment was studied in two wheat (Triticum aestivum L.) cultivars differing in salt tolerance (salt-tolerant Dekang-961 and sensitive Lumai-15). The plants were pre-treated or not with 50 μM 5-azaC and then subjected to salt stress induced by 100 or 150 mM NaCl. Salinity caused reduction in biomass accumulation and increase in malondialdehyde content in root tissues in both cultivars, but less in pre-treated seedlings. The activities of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) in the roots of both cultivars increased during salt stress, but the rate of increase was higher in Dekang-961. Plants treated with 5-azaC had higher root SOD, CAT and POD activities under salt stress than untreated plants. Content of 5-methylcytosine (5mC) decreased in both cultivars under salt stress, and the level of demethylation was higher in Dekang-961 than that in Lumai-15. Moreover, the degree of methylation was lower in both cultivars under salt stress after 5-azaC application compared to only salt-treated groups. These findings suggested that 5-azaC could protect plants from salt stress.

Additional key words

antioxidant enzymes DNA methylation lipid peroxidation 







analysis of variance


catalase (EC






nitroblue tetrazolium


peroxidase (EC


reactive oxygen species


superoxide dismutase (EC


thiobarbituric acid


trichloroacetic acid


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This work was carried out with the financial support from the National Natural Science Foundation of China (No. 30821064) and the Chinese 111 Project (B06018).


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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  1. 1.Key Laboratory of the MOE for Plant Development Biology, College of Life SciencesWuhan UniversityWuhanP.R. China

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