Biologia Plantarum

, Volume 54, Issue 4, pp 614–620 | Cite as

Overexpression of tomato tAPX gene in tobacco improves tolerance to high or low temperature stress

  • W. -H. Sun
  • M. Duan
  • F. Li
  • D. -F. Shu
  • S. Yang
  • Q. -W. Meng
Original Papers


In order to investigate the function of chloroplast ascorbate peroxidase under temperature stress, the thylakoid-bound ascorbate peroxidase gene from tomato leaf (TtAPX) was introduced into tobacco. Transformants were selected for their ability to grow on medium containing kanamycin. RNA gel blot analysis confirmed that TtAPX in tomato was induced by chilling or heat stress. Over-expression of TtAPX in tobacco improved seed germination under temperature stress. Two transgenic tobacco lines showed higher ascorbate peroxidase activity, accumulated less hydrogen peroxide and malondialdehyde than wild type plants under stress condition. The photochemical efficiency of photosystem 2 in the transgenic lines was distinctly higher than that of wild type plants under chilling and heat stresses. Results indicated that the over-expression of TtAPX enhanced tolerance to temperature stress in transgenic tobacco plants.

Additional key words

Lycopersicum esculentum malondialdehyde Nicotiana tabacum photosystem 2 thylakoid-bound ascorbate peroxidase transgenic tobacco 



ascorbate peroxidase


ascorbic acid






initial fluorescence


variable to maximum fluorescence ratio (maximum photochemical efficiency of PS 2)




superoxide radical


phosphate-buffered saline


net photosynthetic rate

PS 2

photosystem 2


relative electrolyte leakage


reactive oxygen species


sodium dodecyl sulfate


superoxide dismutase


standard saline citrate


thylakoid-bound ascorbate peroxidase


Tris-buffered acetate


trichloroacetic acid


tomato thylakoid-bound ascorbate peroxidase gene


wild type plant


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This research was supported by the State Key Basic Research and Development Plan of China (2009CB118500), the Natural Science Foundation of China (30871458) and Program for Changjiang Scholars and Innovative Research Team in University (Grant IRT0635).


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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • W. -H. Sun
    • 1
  • M. Duan
    • 1
  • F. Li
    • 1
  • D. -F. Shu
    • 1
  • S. Yang
    • 1
  • Q. -W. Meng
    • 1
  1. 1.College of Life Science, State Key Laboratory of Crop BiologyShandong Agricultural UniversityTai’anP.R. China

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