Storage and conversion of Eclipta alba synseeds and RAPD analysis of the converted plantlets
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The encapsulated shoot tips and nodal segments of Eclipta alba were stored at 4, 12 and 20 °C under irradiance of 1.5 gmmol m−2 s−1 and high conversion was observed in synseeds stored at 4 °C for 8 weeks. Duration of storage was extended up to 12 weeks by decreasing sucrose concentration in the alginate matrix from 3 to 1 or 2 % and conversion frequency was 71.2–76.1 %. Synseed-derived plantlets survived by 100 % in ex vitro conditions. RAPD analysis revealed uniform amplification profile in donor and synseed derived plantlets.
Additional key wordsalginate matrix encapsulated explants ex vitro transfer genetic uniformity sucrose
encapsulated nodal segment
polymerase chain reaction
random amplified polymorphic DNA
encapsulated shoot tip
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The research was supported by the University Grants Commission, New Delhi. We also thank Mr. Jadab Ghosh for providing technical assistance.
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