Activity of antioxidant enzyme during in vitro organogenesis in Crocus sativus
- 441 Downloads
The effect of various hormonal combinations on regeneration of shoots and roots from meristem-derived callus of Crocus sativus L. and activities of antioxidant enzymes have been studied. The most efficient regeneration occurred with 1.0 mg dm−3 1-naphthaleneacetic acid (NAA) + 1.0 mg dm−3 thidiazuron and 1.0 mg dm−3 NAA + 2.0 mg dm−3 kinetin. For sprouting, regenerated shoot were subcultured on Murashige and Skoog medium containing 1.0 mg dm−3 NAA + 1.0 mg dm−3 benzylaminopurine (BAP). Protein content and superoxide dismutase activity decreased in regenerated shoots and roots and increased in sprouting shoots, while catalase (CAT), peroxidase (POX) and polyphenol oxidase (PPO) activities increased during organogenesis and decreased in sprouting shoots. High CAT and PPO activities were detected in regenerated roots, whereas high POX activity was observed in regenerated shoot.
Additional key wordscatalase peroxidase polyphenol oxidase protein saffron superoxide dismutase
Murashige and Skoog
polyacrylamide gel electrophoresis
picloram (4-amino-3,5-trichloropicolinic acid)
reactive oxygen species
sodium dodecyl sulfate
Unable to display preview. Download preview PDF.
The financial support of this research was provided by University of Tehran. The authors are grateful to the anonymous reviewers for very helpful and valuable advices.
- Aebi, H.: Catalases. — In: Bergmeyer, H.U. (ed.): Methods of Enzymatic Analysis. Vol. 2. Pp. 673–684. Academic Press, New York 1974.Google Scholar
- Basker, D., Negbi, M.: Uses of saffron. — Econ. Bot. 37: 228–236, 1983.Google Scholar
- Benson, E.E.: Do free radicals have a role in plant tissue culture recalcitrance? — In Vitro cell. dev. Biol. Plant 36: 163–170, 2000.Google Scholar
- Ding, B.Z., Bai, S.H., Wu, Y., Fan, X.P.: Induction of callus and regeneration of plantlets from corm of Crocus sativus L. — Acta bot. sin. 23: 434–440, 1981.Google Scholar
- Gaspar, T.: The concept of cancer in in vitro plant cultures and the implication of habituation to hormones and hyperhydricity. — Plant Tissue Cult. Biotechnol. 1: 126–136, 1995.Google Scholar
- Ilahi, I., Jabeen, M., Firdous, N.: Morphogenesis with saffron tissue culture. — J. Plant Physiol. 128: 227–232, 1987.Google Scholar
- Van Loon, L.C., Geelen, J.L.M.C.: The relation of polyphenol oxidase and peroxidase to symptom expression in tobacco var. “Samsun NN“ after infection with tobacco mosaic virus. — Acta phytopathol. Acad. Sci. hung. 6: 9–20, 1971.Google Scholar
- Wendel, J.F., Weeden, N.F.: Visualization and interpretation of plant isozymes. — In: Soltis, D.E., Soltis, P.S. (ed.): Isozymes in Plant Biology. Pp. 4–45. Dioscorides Press, Portland 1989.Google Scholar