Abstracts
An efficient propagation system via somatic embryogenesis and shoot organogenesis and plant regeneration system for endangered species Primulina tabacum Hance was established. Thidiazuron (TDZ) was the key plant growth regulator for inducing somatic embryogenesis and kinetin (KIN) and 6-benzylaminopurine (BAP) were the key cytokinins for inducing shoot organogenesis from leaf explants. TDZ combined with BAP or KIN in the induction Murashige and Skoog medium induced both somatic embryos and adventitious shoots. Leaf explants with abaxial site in contact with the medium induced less somatic embryos or adventitious shoots compared to inversely placed leaf explants and the optimum pH was 6.5–7.0. Secondary somatic embryos or adventitious shoot could be induced from primary somatic embryos using TDZ and BAP. Shoots developed adventitious roots on rooting medium containing 0.5 μM indole-3-butyric acid and 0.2 % activated carbon. Over 90 % of plantlets survived following acclimatization and transfer to potting mixture (sand:Vermiculite:limestone; 1:2:1).
Abbreviations
- BAP:
-
6-benzylaminopurine
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- IBA:
-
indole-3-butyric acid
- KIN:
-
kinetin
- NAA:
-
α-naphthalene acetic acid
- TDZ:
-
thidiazuron
References
Divya, K., Swathi, A.T., Jami, S.K., Kirtip, P.B.: Efficient regeneration from hypocotyl explants in three cotton cultivars. — Biol. Plant. 52: 201–208, 2008.
Faisal, M., Anis, M.: Thidiazurun induced high frequency axillary shoot multiplication in Psoralea corylifolia. — Biol. Plant. 50: 437–440, 2006.
Kumari, G.K., Ganesan, M., Jayabalan, N.: Somatic organogenesis and plant regeneration in Ricinus communis. — Biol. Plant. 52: 17–25, 2008.
Landi, L., Mezzetti, B.: TDZ, auxin and genotype effects on leaf organogenesis in Fragaria. — Plant Cell Rep. 25: 281–288, 2006.
Ledbetter, D.I., Preece, J.E.: Thidiazuron stimulates adventitious shoot production from Hydrangea quercifolia Bartr. leaf explants. — Sci. Hort. 101: 121–126, 2004.
Ma, G.H., Li, Y., Jiao, G.L., Fu, X.P., Lin, Y.R.: Adventitious shoots and callus formation in vitro from young leaves of Melastoma affine. — Floricult. Ornam. Biotechnol. 1: 27–29, 2007.
Ma, G.H., Wu, G.J.: Direct shoot organogenesis from cotyledon explants in Ochna integerrima. — Propag. Ornam. Plant 6: 145–148, 2006.
Ma, G.H., Xu, Q.S.: Induction of somatic embryogenesis and adventitious shoot formation from immature leaves of cassava. — Plant Cell Tissue Organ Cult. 70: 281–288, 2002.
Ma, G.H., Xu, Q.S., Xian, Y.L.: [Direct primary somatic embryogenesis and shoot formation in Manihot esculenta.] — Acta bot. sin. 40: 503–507, 1998. [In Chin.]
Mathews, H., Schopke, C., Carcamo, R., Chavarriaga, P., Fauquet, C., Beachy, R.N.: Improvement of somatic embryogenesis and plant-recovery in cassava. — Plant Cell Rep. 12: 328–333, 1993.
Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue culture. — Physiol. Plant. 15: 473–497, 1962.
Murch, S.J., Saxena, P.K.: The role of proline in thidiazuron induced somatic embryogenesis of peanut. — In Vitro cell. dev. Biol. Plant 5: 102–105, 1999.
Murthy, B.N.S., Saxena, P.K.: Somatic embryogenesis and plant regeneration of neem (Azadirachta indica A. Juss). — Plant Cell Rep. 17: 469–475, 1998.
Nhut, D.T., Vu, N.H., Xuan, L.T.T.: The application of a bag culture system for the in vitro propagation of Sinningia spp. — Propag. Ornam. Plant 5: 45–50, 2005.
Raemakers, C.J.J.M., Amati, M., Staritsky, G., Jacobsen, E., Visser, R.G.F.: Cyclic somatic embryogenesis and plant regeneration in cassava. — Ann. Bot. 71: 289–294, 1993.
Raemakers, K., Jacobsen, E., Visser, R.: The use of somatic embryogenesis for plant propagation in cassava. — Mol. Biotechnol. 14: 215–221, 2000.
Ren, H., Peng, S.L., Zhang, J.X., Jian, S.G., Wei, Q., Zhang, Q.M., Liu, N., Li, S.J., Chen, W.B., Zhuang, Y.Z.: [The ecological and biological characteristics of an endangered plant, Primulina tabacum Hance.] — Acta ecol. sin. 23: 1012–1017, 2003. [In Chin.]
Scaramuzzi, F., Apollonio, G., D’Emerico, S.: Adventitious shoot regeneration from Sinningia speciosa leaf discs in vitro and stability of ploidy level in subcultures. — In Vitro cell. dev. Biol. Plant 35: 217–221, 1999.
Tang, Z.H., Lin, H.H., Shi, L., Chen, W.L.: Rapid in vitro multiplication of Chirita longgangensis WT Wang: an endemic and endangered gesneriaceae species in China. — HortScience 42: 638–641, 2007.
Xing, F.W.: [Rare Plants of China.] — Hunan Education Publisher, Changsha 2005. [In Chin.]
Acknowledgement
Supports from the Corporation Program of National Basic Research Program of China (973 Program) (2007CB411600), the Program for Tropical & Subtropical Plant Germplasm Construction of Guangdong Province (2005B60301001, 2006B60101034) and Guangdong Scientific Program (2007A060306011) are gratefully acknowledged.
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Ma, G.H., He, C.X., Ren, H. et al. Direct somatic embryogenesis and shoot organogenesis from leaf explants of Primulina tabacum . Biol Plant 54, 361–365 (2010). https://doi.org/10.1007/s10535-010-0064-5
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DOI: https://doi.org/10.1007/s10535-010-0064-5