Genetic diversity in important members of Cucurbitaceae using isozyme, RAPD and ISSR markers
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Biochemical and molecular markers have been used on eleven species of Cucurbitaceae collected from lower Gangetic plains. Six enzyme systems were selected. Among 40 primers examined, 14 random amplified polymorphic DNA (RAPD) and 10 inter-simple sequence repeat (ISSR) primers were selected for the analysis. Generated RAPD (100) and ISSR (100) fragments showed high variations among the species. Jaccard similarity coefficients were used for the evaluation of pairwise genetic divergence; cluster analysis of the similarity matrices was performed to estimate interspecific diversity. Further, principal coordinate analysis was performed to evaluate the resolving power of the three marker systems to differenciate among the species.
Additional key wordscluster analysis Jaccard similarity coefficient molecular marker primer
inter-simple sequence repeat
polymerase chain reaction
random amplified polymorphic DNA
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B. Sikdar is gratefully acknowledged to TWAS for financial support to the postdoctoral research.
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