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Biologia Plantarum

, 53:685 | Cite as

Comparison of drought-induced polypeptides and ion leakage in three tomato cultivars

  • R. Roy
  • V. Agrawal
  • S. C. Gupta
Original Papers

Abstract

An attempt has been made to determine if drought-induced proteins could be used as a selection marker to differentiate between tolerant and sensitive cultivars. Three Indian tomato (Lycopersicon esculentum Mill.) cultivars (Pusa Ruby, Arka Vikas and Pusa Early Dwarf) were subjected to drought stress in vivo as well as in vitro and the pattern of polypeptide expression was determined using one-dimensional SDS-PAGE. In all the three cultivars, a new 29 kDa polypeptide accumulated in leaves, in response to gradual drought stress and its accumulation was fastest in Pusa Ruby. Drought stress also resulted in an increase in ion leakage from leaf discs of all the three cultivars but the rate was lower in Pusa Ruby than in other two. Therefore, it was concluded that Pusa Ruby is most tolerant to drought stress among the three tomato cultivars investigated.

Additional key words

drought tolerance Lycopersicon esculentum mannitol SDS-PAGE water stress 

Abbreviations

ABA

abscisic acid

BA

N6-benzyladenine

BSA

bovine serum albumin

DTT

dithiothreitol

EDTA

ethylenediaminetetra-acetic acid

IAA

indole-3-acetic acid

LEA

late embryogenesis abundant

MS

Murashige and Skoog

PAGE

polyacrylamide gel electrophoresis

SDS

sodium dodecyl sulphate

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  1. 1.Department of BotanyUniversity of DelhiDelhiIndia
  2. 2.Dr. B.R. Ambedkar Centre for Biomedical ResearchUniversity of DelhiDelhiIndia

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