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QTLs mapping of physiological traits related to salt tolerance in young rice seedlings

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Biologia Plantarum

Abstract

Oryza sativa L. F2 population and F2:3 derived from a cross between salt tolerance cv. Tarommahali and salt sensitive cv. Khazar were used in this study. A linkage map based on F2 population was constructed (74 SSR markers on 192 individuals), which covered a total of 1231.50 cM with an average two locus interval of 19.83 cM. Two QTLs related to Na+/K+ ratio were found on chromosome 3 and 6. qDM-3 and qDM-8 (for dry mass of shoot) are major QTLs with very large effects explained 20.90 and 17.72 % of the total phenotypic variance, respectively. Major locus for DM (qDM-3) was bracketed by RM1022 — RM6283 spread over 13.6 cM on chromosome 3. Major part of the variability for standard tolerance ranking (STR) was explained by the qSTR-6 flanked by RM3727 — RM340 on chromosome 6, which exhibited phenotypic variance of 17.25 % and peak likelihood ratio (LR) of 17.51. The length of this QTL is 8.8 cM and identification of any tightly linked markers in this region will serve as a candidate gene for fine-mapping. qSTR-3 overlapped with qNA-3 and qNAK-3. The qSTR-3 may contain a new major gene for salt stress tolerance at seedling stage in rice. Major QTLs identified in this paper, after fine-mapping, could be used for marker assisted selection.

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Abbreviations

CIM:

composite interval mapping

LOD:

logarithmic odds

LR:

likelihood ratio

MAS:

marker assisted selection

QTL:

quantitative trait locus

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Authors and Affiliations

  1. Department of Plant Production, Gonbad High Education Center, Gorgan University of Agriculture Science and Natural Resource, Shahid Fallahi Ave, Gonbad, 49717-99151, Iran

    H. Sabouri

  2. Faculty of Agriculture Sciences, Isfahan University of Technology, Isfahan, 84156-83111, Iran

    A. M. Rezai

  3. Researchers in Plant Breeding, Rice Research Institute of Iran, Rasht, 41996-13475, Iran

    A. Moumeni, A. Kavousi & M. Katouzi

  4. Faculty of Agriculture Sciences, University of Guilan, Rasht, 41635-1314, Iran

    A. Sabouri

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  1. H. Sabouri
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  2. A. M. Rezai
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  3. A. Moumeni
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  4. A. Kavousi
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  5. M. Katouzi
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  6. A. Sabouri
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Correspondence to H. Sabouri.

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Acknowledgements: This research was supported by Isfahan University of Technology and Rice Research Institute of Iran. The authors are grateful to Ahmadreza Dadras and Ali Akbar Ebadi for their excellent technical assistance and greenhouse management.

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Sabouri, H., Rezai, A.M., Moumeni, A. et al. QTLs mapping of physiological traits related to salt tolerance in young rice seedlings. Biol Plant 53, 657–662 (2009). https://doi.org/10.1007/s10535-009-0119-7

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  • DOI: https://doi.org/10.1007/s10535-009-0119-7

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