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Biologia Plantarum

, Volume 53, Issue 4, pp 657–662 | Cite as

QTLs mapping of physiological traits related to salt tolerance in young rice seedlings

  • H. Sabouri
  • A. M. Rezai
  • A. Moumeni
  • A. Kavousi
  • M. Katouzi
  • A. Sabouri
Original Papers

Abstract

Oryza sativa L. F2 population and F2:3 derived from a cross between salt tolerance cv. Tarommahali and salt sensitive cv. Khazar were used in this study. A linkage map based on F2 population was constructed (74 SSR markers on 192 individuals), which covered a total of 1231.50 cM with an average two locus interval of 19.83 cM. Two QTLs related to Na+/K+ ratio were found on chromosome 3 and 6. qDM-3 and qDM-8 (for dry mass of shoot) are major QTLs with very large effects explained 20.90 and 17.72 % of the total phenotypic variance, respectively. Major locus for DM (qDM-3) was bracketed by RM1022 — RM6283 spread over 13.6 cM on chromosome 3. Major part of the variability for standard tolerance ranking (STR) was explained by the qSTR-6 flanked by RM3727 — RM340 on chromosome 6, which exhibited phenotypic variance of 17.25 % and peak likelihood ratio (LR) of 17.51. The length of this QTL is 8.8 cM and identification of any tightly linked markers in this region will serve as a candidate gene for fine-mapping. qSTR-3 overlapped with qNA-3 and qNAK-3. The qSTR-3 may contain a new major gene for salt stress tolerance at seedling stage in rice. Major QTLs identified in this paper, after fine-mapping, could be used for marker assisted selection.

Additional key words

marker-assisted selection Oryza sativa SSR 

Abbreviations

CIM

composite interval mapping

LOD

logarithmic odds

LR

likelihood ratio

MAS

marker assisted selection

QTL

quantitative trait locus

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • H. Sabouri
    • 1
  • A. M. Rezai
    • 2
  • A. Moumeni
    • 3
  • A. Kavousi
    • 3
  • M. Katouzi
    • 3
  • A. Sabouri
    • 4
  1. 1.Department of Plant Production, Gonbad High Education CenterGorgan University of Agriculture Science and Natural ResourceGonbadIran
  2. 2.Faculty of Agriculture SciencesIsfahan University of TechnologyIsfahanIran
  3. 3.Researchers in Plant BreedingRice Research Institute of IranRashtIran
  4. 4.Faculty of Agriculture SciencesUniversity of GuilanRashtIran

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