Biologia Plantarum

, 53:583 | Cite as

The promoter-elements of some abiotic stress-inducible genes from cereals interact with a nuclear protein from tobacco

  • A. Roychoudhury
  • D. N. Sengupta
Brief Communication


In this communication, we report the binding of abscisic acid responsive elements (ABREs) of rice Osem, namely motif A and motif B, with a cognate trans-acting factor present in the nuclear extract of tobacco leaf. The binding is specific as both the complexes were disrupted with an excess of homologous non-radioactive DNA like motif A or motif B themselves or with cis-elements of rice Rab16A, motif I (ABRE) and motif IIa (non-ACGT ABRE-like sequences). Four tandem repeats of ABRE from wheat Em (4X ABRE) or two tandem repeats of Em ABRE, plus two copies of coupling element (CE1) from barley HVA22 (2X ABRC), also showed specific complexes, that were competed out by an excess of homologous competitors like motif I, motif IIa, motif A, motif B, 4X ABRE and 2X ABRC, but not by the unrelated 4X DRE sequence. Elution of the protein from all the complexes showed a single 26 kDa polypeptide band. Introgression of two of the above synthetic promoters 4X ABRE and 2X ABRC, each fused with minimal promoter of cauliflower mosaic virus 35S (CaMV 35S), could induce the expression of the reporter gene β-glucuronidase (gus) in transgenic tobacco in response to high NaCl concentration, dehydration or abscisic acid, but not at the constitutive level, proving that they can be used as efficient stress-inducible promoters. Our work shows both in vivo and in vitro activity of the promoters from monocot genes in the model dicot plant tobacco.

Additional key words

abscisic acid responsive complex abscisic acid responsive elements barley coupling elements Hordeum vulgare Nicotiana tabacum Oryza sativa rice transgenic tobacco 



abscisic acid responsive element


abscisic acid responsive complex

CaMV 35S

cauliflower mosaic virus 35S


coupling element


dehydration responsive element


electrophoretic mobility shift assay


β-glucuronidase gene


polyethylene glycol


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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  1. 1.Department of BotanyBose InstituteKolkataIndia
  2. 2.Department of Botany, Plant Molecular Biology and Biotechnology LaboratoryUniversity of CalcuttaKolkataIndia

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