Advertisement

Biologia Plantarum

, 53:583 | Cite as

The promoter-elements of some abiotic stress-inducible genes from cereals interact with a nuclear protein from tobacco

  • A. Roychoudhury
  • D. N. Sengupta
Brief Communication

Abstract

In this communication, we report the binding of abscisic acid responsive elements (ABREs) of rice Osem, namely motif A and motif B, with a cognate trans-acting factor present in the nuclear extract of tobacco leaf. The binding is specific as both the complexes were disrupted with an excess of homologous non-radioactive DNA like motif A or motif B themselves or with cis-elements of rice Rab16A, motif I (ABRE) and motif IIa (non-ACGT ABRE-like sequences). Four tandem repeats of ABRE from wheat Em (4X ABRE) or two tandem repeats of Em ABRE, plus two copies of coupling element (CE1) from barley HVA22 (2X ABRC), also showed specific complexes, that were competed out by an excess of homologous competitors like motif I, motif IIa, motif A, motif B, 4X ABRE and 2X ABRC, but not by the unrelated 4X DRE sequence. Elution of the protein from all the complexes showed a single 26 kDa polypeptide band. Introgression of two of the above synthetic promoters 4X ABRE and 2X ABRC, each fused with minimal promoter of cauliflower mosaic virus 35S (CaMV 35S), could induce the expression of the reporter gene β-glucuronidase (gus) in transgenic tobacco in response to high NaCl concentration, dehydration or abscisic acid, but not at the constitutive level, proving that they can be used as efficient stress-inducible promoters. Our work shows both in vivo and in vitro activity of the promoters from monocot genes in the model dicot plant tobacco.

Additional key words

abscisic acid responsive complex abscisic acid responsive elements barley coupling elements Hordeum vulgare Nicotiana tabacum Oryza sativa rice transgenic tobacco 

Abbreviations

ABRE

abscisic acid responsive element

ABRC

abscisic acid responsive complex

CaMV 35S

cauliflower mosaic virus 35S

CE

coupling element

DRE

dehydration responsive element

EMSA

electrophoretic mobility shift assay

gus

β-glucuronidase gene

PEG

polyethylene glycol

References

  1. An, G.: Binary ti vectors for plant transformation and promoter analysis. — Methods Enzymol. 153: 292–293, 1987.CrossRefGoogle Scholar
  2. Blum, H., Beier, H., Gross, H.J.: Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gel. — Electrophoresis 8: 93–99, 1987.CrossRefGoogle Scholar
  3. Bradford, M.M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. — Anal. Biochem. 72: 248–254, 1976.CrossRefPubMedGoogle Scholar
  4. Busk, P.K., Pages, M.: Regulation of abscisic acid-induced transcription. — Plant mol. Biol. 37: 425–435, 1998.CrossRefPubMedGoogle Scholar
  5. Cherian, S., Reddy, M.P., Ferreira, R.B.: Transgenic plants with improved dehydration-stress tolerance: progress and future prospects. — Biol. Plant. 50: 481–495, 2006.CrossRefGoogle Scholar
  6. Cousson, A.: Involvement of phospholipase C-independent calcium-mediated abscisic acid signalling during Arabidopsis response to drought. — Biol. Plant. 53: 53–62, 2009.CrossRefGoogle Scholar
  7. Furtado, A., Henry, R.J.: The wheat Em promoter drives reporter gene expression in embryo and aleurone tissue of transgenic barley and rice. — Plant Biotechnol. J. 3: 421–434, 2005.CrossRefPubMedGoogle Scholar
  8. Guiltinan, M.J., Marcotte, W.R., Jr., Quatrano, R.S.: A plant leucine zipper protein that recognizes an abscisic acid response element. — Science 250: 267–271, 1990.CrossRefPubMedGoogle Scholar
  9. Hattori, T., Terada, T., Hamasuna, S.: Regulation of the Osem gene by abscisic acid and the transcriptional activator VP1: analysis of cis-acting promoter elements required for regulation by abscisic acid and VP1. — Plant J. 7: 913–925, 1995.CrossRefPubMedGoogle Scholar
  10. Hattori, T., Totsuka, M., Hobo, T., Kagaya, Y., Yamamoto-Toyoda, A.: Experimentally determined sequence requirement of ACGT-containing abscisic acid response element. — Plant Cell Physiol. 43: 136–140, 2002.CrossRefPubMedGoogle Scholar
  11. Hobo, T., Asada, M., Kowyama, Y., Hattori, T.: ACGTcontaining abscisic acid response element (ABRE) and coupling element 3 (CE3) are functionally equivalent. — Plant J. 19: 679–689, 1999.CrossRefPubMedGoogle Scholar
  12. Huang, C., Guo, T., Zheng, S.C., Feng, Q.L., Liang, J.H., Li, L.: Increased cold tolerance in Arabidopsis thaliana transfromed with Choristoneura fumiferana glutahione Strasferase gene. — Biol. Plant. 53: 183–187, 2009.CrossRefGoogle Scholar
  13. Ishige, F., Takaichi, M., Foster, R., Chua, N.H., Oeda, K.: A Gbox motif (GCCACGTGCC) tetramer confers high-level constitutive expression in dicot and monocot plants. — Plant J. 18: 443–448, 1999.CrossRefGoogle Scholar
  14. Katagiri, F., Lam, E., Chua, N.H.: Two tobacco DNA-binding proteins with homology to the nuclear factor CREB. — Nature 340: 727–730, 1989.CrossRefPubMedGoogle Scholar
  15. Kosova, K., Vitámvás, P., Prášil, I.T.: The role of dehydrins in plant response to cold. — Biol. Plant. 51: 601–617, 2007.CrossRefGoogle Scholar
  16. Longhurst, T., Lee, E., Hinde, R., Brady, C., Speirs, J.: Structure of the tomato Adh2 gene and Adh2 pseudogenes, and a study of Adh2 gene expression in fruit. — Plant mol. Biol. 26: 1073–1084, 1994.CrossRefPubMedGoogle Scholar
  17. Marcotte, W.R., Jr., Russell, S.H., Quatrano, R.S.: Abscisic acid-responsive sequences from the em gene of wheat. — Plant Cell 1: 969–976, 1989.CrossRefPubMedGoogle Scholar
  18. Mukherjee, K., Choudhury, A.R., Gupta, B., Gupta, S., Sengupta, D.N.: An ABRE-binding factor, OSBZ8, is highly expressed in salt tolerant cultivars than in salt sensitive cultivars of indica rice. — BMC Plant Biol. 6: 18, 2006.CrossRefPubMedGoogle Scholar
  19. Mundy, J., Chua, N.H.: Abscisic acid and water-stress induce the expression of a novel rice gene. — EMBO J. 7: 2279–2286, 1988.PubMedGoogle Scholar
  20. Nakagawa, H., Ohmiya, K., Hattori, T.: A rice bZIP protein, designated OSBZ8, is rapidly induced by abscisic acid. — Plant J. 9: 217–227, 1996.CrossRefPubMedGoogle Scholar
  21. Nantel, A., Quatrano, R.S.: Characterization of three rice basic/leucine zipper factors, including two inhibitors of EmBP-1 DNA binding activity. — J. biol. Chem. 271: 31296–31305, 1996.CrossRefPubMedGoogle Scholar
  22. Oeda, K., Salinas, J., Chua, N.H.: A tobacco bZip transcription activator (TAF-1) binds to a G-box-like motif conserved in plant genes. — EMBO J. 10: 1793–1802, 1991.PubMedGoogle Scholar
  23. Ono, A., Izawa, T., Chua, N.H., Shimamoto, K.: The rab16B promoter of rice contains two distinct abscisic acidresponsive elements. — Plant Physiol. 112: 483–491, 1996.CrossRefPubMedGoogle Scholar
  24. RoyChoudhury, A., Roy, C., Sengupta, D.N.: Transgenic tobacco plants overexpressing the heterologous lea gene Rab16A from rice during high salt and water deficit display enhanced tolerance to salinity stress. — Plant Cell Rep. 26: 1839–1859, 2007.CrossRefPubMedGoogle Scholar
  25. RoyChoudhury, A., Gupta, B., Sengupta, D.N.: Trans-acting factor designated OSBZ8 interacts with both typical abscisic acid responsive elements as well as abscisic acid responsive element-like sequences in the vegetative tissues of indica rice cultivars. — Plant Cell Rep. 27: 779–794, 2008.CrossRefPubMedGoogle Scholar
  26. Sambrook, J., Russell, D.W.: Molecular Cloning: A Laboratory Manual. 3rd Ed. — Cold Spring Harbor Laboratory Press, Cold Spring Harbor 2001.Google Scholar
  27. Shen, Q., Ho, T.H.D.: Functional dissection of an abscisic acid (ABA)-inducible gene reveals two independent ABAresponsive complexes, each containing a G-box and a novel cis-acting element. — Plant Cell 7: 295–307, 1995.CrossRefPubMedGoogle Scholar
  28. Shen, Q., Zhang, P., Ho, T.H.D.: Modular nature of abscisic acid (ABA) response complexes: composite promoter units that are necessary and sufficient for ABA induction of gene expression in barley. — Plant Cell 8: 1107–1119, 1996.CrossRefPubMedGoogle Scholar
  29. Singh, K.B.: Transcriptional regulation in plants: the importance of combinatorial control. — Plant Physiol. 118: 1111–1120, 1998.CrossRefPubMedGoogle Scholar
  30. Skriver, K., Olsen, F.L., Rogers, J.C., Mundy, J.: Cis-acting DNA elements responsive to gibberellin and its antagonist abscisic acid. — Proc. nat. Acad. Sci. USA. 88: 7266–7270, 1991.CrossRefPubMedGoogle Scholar
  31. Yamaguchi-Shinozaki, K., Mundy, J., Chua, N.H.: Four tightly linked rab genes are differentially expressed in rice. — Plant mol. Biol. 14: 29–39, 1990.CrossRefPubMedGoogle Scholar
  32. Yamaguchi-Shinozaki, K., Shinozaki, K.: A novel cis-acting element in an Arabidopsis gene is involved in responsiveness to drought, low-temperature, or high-salt stress. — Plant Cell 6: 251–264, 1994.CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  1. 1.Department of BotanyBose InstituteKolkataIndia
  2. 2.Department of Botany, Plant Molecular Biology and Biotechnology LaboratoryUniversity of CalcuttaKolkataIndia

Personalised recommendations