Biologia Plantarum

, 53:565 | Cite as

Effect of esculetin on activities of pumpkin glutathione S-transferases and growth of pumpkin seedlings

  • M. D. Hossain
  • M. Fujita
Brief Communication


The effect of esculetin and some related coumarins (coumarin, 7-hydroxycoumarin and scopoletin) on growth of pumpkin (Cucurbita maxima Duch.) seedlings and the activities of pumpkin glutathione S-transferases (GSTs) were investigated. Coumarin and esculetin affected the growth of seedlings. The hypocotyls of affected seedlings became weak and bent at the mid region, roots became very soft with brownish discoloration, and finally seedlings died. Among the compounds tested, only esculetin inhibited CmGSTU3 and CmGSTU2 activities measured with 1-chloro-2,4-dinitrobenzene (CDNB) and at a concentration of 22 µM, it inhibited the activity of CmGSTU3 by 50 %.

Additional key words

coumarins Cucurbita maxima detoxification fluorescent substance inhibitor 



1-chloro-2,4- dinitrobenzene




glutathione S-transferase


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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  1. 1.Department of Plant Sciences, Faculty of AgricultureKagawa UniversityKagawaJapan

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