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Abscisic acid and auxin accumulation in Catasetum fimbriatum roots growing in vitro with high sucrose and mannitol content

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Biologia Plantarum

Abstract

Endogenous contents of indolyl-3-acetic acid (IAA) and abscisic acid (ABA) were quantified in excised roots of Catasetum fimbriatum (Orchidaceae) cultured in vitro on solidified Vacin and Went medium with 1, 2, 4, 6, 8 and 10 % sucrose, as well as 2 % sucrose plus mannitol. Maximum root growth was observed in media with 4 % sucrose and 2 % sucrose plus 2.2 % mannitol, suggesting that a moderate water or osmotic stress promotes orchid root growth. Contents of both ABA and IAA increased in parallel to increasing sucrose concentration and a correlation between root elongation and the ABA/IAA ratio was observed. Incubating isolated C. fimbriatum roots with radiolabeled tryptophan, we showed an accumulation of IAA and its conjugates.

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Abbreviations

ABA:

abscisic acid

ELISA:

enzyme linked immunosorbent assay

HPLC:

high performance liquid chromatography

IAA:

indole-3-acetic acid

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Authors and Affiliations

  1. Department of Biological Sciences (LCB), Escola Superior de Agricultura “Luiz de Queiroz” (ESALQ), Universidade de São Paulo, 13418-900, Piracicaba, SP, Brazil

    L. E. P. Peres & A. Zsögön

  2. Departament of Botany, Institute of Biosciences, Universidade de São Paulo, 05422-970, São Paulo, SP, Brazil

    G. B. Kerbauy

Authors
  1. L. E. P. Peres
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  2. A. Zsögön
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  3. G. B. Kerbauy
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Corresponding author

Correspondence to L. E. P. Peres.

Additional information

Acknowledgements: We thank FAPESP and CNPq (Brazil) for financial support and Dr. B. Sotta (Université Paris VI) for the donation of the antibodies and conjugates for ELISA.

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Peres, L.E.P., Zsögön, A. & Kerbauy, G.B. Abscisic acid and auxin accumulation in Catasetum fimbriatum roots growing in vitro with high sucrose and mannitol content. Biol Plant 53, 560–564 (2009). https://doi.org/10.1007/s10535-009-0101-4

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  • DOI: https://doi.org/10.1007/s10535-009-0101-4

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