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Biologia Plantarum

, Volume 53, Issue 1, pp 145–150 | Cite as

Differential expression of LEA proteins in two genotypes of mulberry under salinity

  • G. Jyothsnakumari
  • M. Thippeswamy
  • G. Veeranagamallaiah
  • C. Sudhakar
Brief Communication

Abstract

The relative water content (RWC), cell membrane integrity, protein pattern and the expression of late embryogenesis abundant proteins (LEA; group 1, 2, 3 and 4) under different levels of salt stress (0, 1.0, 1.5 and 2.0 % NaCl) were investigated in mulberry (Morus alba L.) cultivars (S1 and ATP) with contrasting salt tolerance. RWC and membrane integrity decreased with increase in NaCl concentration more in cv. ATP than in cv. S1. SDS-PAGE protein profile of mulberry leaves after the NaCl treatments showed a significant increase in 35, 41, 45 and 70 kDa proteins and significant decrease in 14.3, 18, 23, 28, 30, 42, 47 and 65 kDa proteins. Exposure of plants to NaCl resulted in higher accumulation of LEA proteins in S1 than ATP. The maximum content of LEA (group 3 and 4) was detected in S1 at 2.0 % NaCl, which correlates with its salt tolerance.

Additional key words

cell membrane stability Morus alba NaCl stress RWC 

Abbreviations

ABA

abscisic acid

DHN

dehydrin

LEA

late embryogenesis abundant

PR

pathogen related

RAB

responsive to ABA

SDS-PAGE

sodium dodecyl sulfate polyacrylamide gel electrophoresis

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • G. Jyothsnakumari
    • 1
  • M. Thippeswamy
    • 2
  • G. Veeranagamallaiah
    • 2
  • C. Sudhakar
    • 3
  1. 1.Department of BotanyAcharya Nagarjuna UniversityNagarjuma NagarIndia
  2. 2.Department of BotanySri Krishnadevaraya UniversityAnantapurIndia
  3. 3.BiotechnologySri Krishnadevaraya UniversityAnantapurIndia

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