Differential expression of LEA proteins in two genotypes of mulberry under salinity
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The relative water content (RWC), cell membrane integrity, protein pattern and the expression of late embryogenesis abundant proteins (LEA; group 1, 2, 3 and 4) under different levels of salt stress (0, 1.0, 1.5 and 2.0 % NaCl) were investigated in mulberry (Morus alba L.) cultivars (S1 and ATP) with contrasting salt tolerance. RWC and membrane integrity decreased with increase in NaCl concentration more in cv. ATP than in cv. S1. SDS-PAGE protein profile of mulberry leaves after the NaCl treatments showed a significant increase in 35, 41, 45 and 70 kDa proteins and significant decrease in 14.3, 18, 23, 28, 30, 42, 47 and 65 kDa proteins. Exposure of plants to NaCl resulted in higher accumulation of LEA proteins in S1 than ATP. The maximum content of LEA (group 3 and 4) was detected in S1 at 2.0 % NaCl, which correlates with its salt tolerance.
Additional key wordscell membrane stability Morus alba NaCl stress RWC
late embryogenesis abundant
responsive to ABA
sodium dodecyl sulfate polyacrylamide gel electrophoresis
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- Dure, L.: The LEA proteins of higher plants.-In: Verma, D.P.S. (ed.): Control of Plant Gene Expression. Pp. 325–369. CRC Press, Boca Raton 1992.Google Scholar
- Dure, L.: Structural motifs in LEA proteins of higher plants.-In: Close, T.J., Bray, E.A. (ed.): Response of Plants to Cellular Dehydration during Environmental Stress. Pp. 48–61. American Society of Plant Physiologists, Rockville 1993.Google Scholar
- Jayaprakash, T.L., Ramamohan, G., Krishnaprasad, B.T., Kumar, G., Prasad. T.G., Mathew, M.K., Udaya Kumar, M.: Genotypic variability in differential expression of lea2 and lea3 genes and proteins in response to salinity stress in finger millet (Eleusine coracana Gaertn.) and rice (Oryza sativa L.) seedlings.-Ann. Bot. 82: 513–522, 1998.CrossRefGoogle Scholar
- Jyothsnakumari, G.: Studies on biochemical responses and proteome analysis of two high yielding genotypes of mulberry (Morus alba L.) with differential salt sensitivity.-Ph.D thesis, Sri Krishnadevaraya University, Anantapur, 2005.Google Scholar
- Kumari, G.J., Reddy, A.M., Naik, S.T., Kumar, S.G., Prasanthi, J., SriRanganayakulu, G., Reddy, P.C., Sudhakar, C.: Jasmonic acid induced changes in protein pattern, antioxidative enzyme activity and peroxidase isozyme in peanut (Arachis hypogaea L.) seedlings.-Biol. Plant. 50: 219–226, 2006.CrossRefGoogle Scholar
- Laemmli, U.K.: Cleavage of structural protein during the assembly of the head of the bacteriophage T4.-Nature 83: 90–94, 1970.Google Scholar
- Pareek, A., Singla, S.L., Grover, A.: Salt responsive proteins/genes in crop plants.-In: P.K. Jaiwal, R.P. Singh, A. (ed.): Strategies for Improving Salt Tolerance in Higher Plants. Pp. 365–391. Gulati Oxford and IBH Publication Co., New Delhi 1997.Google Scholar
- Singh, N.K., Nelson, D.E., La Rosa, P.S., Bracker, C.E., Handa, A.K., Hasegawa, P.M., Bressan, R.A.: Osmotin: a protein associated with osmotic stress adaptation in plant cells.-In: Cherry, J.H. (ed.): Environmental Stress in Plants. Pp. 67–87. Springer-Verlag, Berlin 1989.Google Scholar