Exploration on the vacuum infiltration transformation of pakchoi
- 93 Downloads
Agrobacterium tumefaciens mediated vacuum infiltration transformation in planta has been established in pakchoi, a kind of Chinese cabbage, but the transformation frequency in harvested seeds has varied in the range of 0.5 to 3.0 × 10−4 over several years and is much lower than the transformation frequency in Arabidopsis thaliana. To understand that, the distribution and vitality changes of A. tumefaciens in plant tissues were examined. Results revealed that there was a majority of A. tumefaciens in the flower compared with that in the stem and in the leaf at all times after infiltration. As fact of transformants in the upper part of the treated plant (T0) stalk and fact of the survival of A. tumefaciens in the plant were proved, possibilities of optimizing the transformation conditions to increase the transformation frequency in pakchoi was discussed.
Additional key wordsAgrobacterium tumefaciens Arabidopsis Brassica rapa ssp. chinensis
Unable to display preview. Download preview PDF.
- Bechtold, N., Ellis, J., Pelletier, G.: In planta agrobacteria mediated gene transfer by infiltration of adult Arabidopsis thaliana plants.-Compt. rend. Acad. Sci. Paris Life Sci. 316: 1194–1199, 1993.Google Scholar
- Fang, Z.D.: Method for Study of Plant Disease. 3rd Ed.-China Agricultural Press, Beijing 1998.Google Scholar
- Liu, F., Cao, M.Q., Yao, L., Robaglia, C., Tourneur, C.: In planta transformation of pakchoi (Brassica campestris L. ssp. chinensis) by infiltration of adult plants with Agrobacterium.-Acta Hort. 467: 187–192, 1998.Google Scholar
- Sambrook, J., Fritsch, E.F., Maniatis, T.: Molecular Cloning: a Laboratory Manual. 2nd Ed.-Cold Spring Harbor Laboratory Press, New York 1989.Google Scholar
- Trieu, A.T., Burleigh, S.H., Kardailsky, L.V., Maldonado-Mendoza, L.E., Versaw, W.K., Blaylock, L.A., Shin, H., Chiou, T.J., Dewbre, G.R., Weigel, D., Harrison, M.J.: Transformation of Medicago Truncatula via infiltration of seedlings or flowering plants with Agrobacterium.-Plant J. 22: 531–541, 2000.PubMedCrossRefGoogle Scholar