Abstract
Agrobacterium tumefaciens mediated vacuum infiltration transformation in planta has been established in pakchoi, a kind of Chinese cabbage, but the transformation frequency in harvested seeds has varied in the range of 0.5 to 3.0 × 10−4 over several years and is much lower than the transformation frequency in Arabidopsis thaliana. To understand that, the distribution and vitality changes of A. tumefaciens in plant tissues were examined. Results revealed that there was a majority of A. tumefaciens in the flower compared with that in the stem and in the leaf at all times after infiltration. As fact of transformants in the upper part of the treated plant (T0) stalk and fact of the survival of A. tumefaciens in the plant were proved, possibilities of optimizing the transformation conditions to increase the transformation frequency in pakchoi was discussed.
References
Azpiroz-Leehan, R., Feldmann, K.A.: T-DNA insertion mutagenesis in Arabidopsis: going back and forth.-Trends Genet. 13: 152–156, 1997.
Bechtold, N., Ellis, J., Pelletier, G.: In planta agrobacteria mediated gene transfer by infiltration of adult Arabidopsis thaliana plants.-Compt. rend. Acad. Sci. Paris Life Sci. 316: 1194–1199, 1993.
Bechtold, N., Jaudeau, B., Jolivet, S., Maba, B., Vezon, D., Voisin, R., Pelletier, G.: The maternal chromosome set is the target of the T-DNA in the in planta transformation of Arabidopsis thaliana.-Genetics 155: 1875–1887, 2000.
Cao, M.Q., Liu, F., Yao, L., Bouchez, D., Tourneur, C., Li, Y., Robaglia, C.: Transformation of pakchoi (Brassica rapa L. ssp. chinensis) by Agrobacterium infiltration.-Mol. Breed. 6: 67–72, 2000.
Chang, S.S., Park, S.K., Kim, B.C., Kang, B.J., Kim, D.U., Nam, H.G.: Stable genetic transformation of Arabidopsis thaliana by Agrobacterium inoculation in planta.-Plant J. 5: 551–558, 1994.
Chung, M.H., Chen, M.K., Pan, S.M.: Floral spray transformation can efficiently generate Arabidopsis transgenic plants.-Transgenic Res. 9: 471–476, 2000.
Clough, S.J., Bent, A.F.: Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana.-Plant J. 16: 735–743, 1998.
Clough, S.J., Fengler, K.A., Yu, I.C., Lippok, B., Roger, K.S., Jr., Bent, A.F.: The Arabidopsis dnd1 “defense no death” gene encodes a mutated cyclic nucleotide-gate ion channel.-Proc. nat. Acad. Sci. USA 97: 9323–9328, 2000.
Curtis, I.S., Nam, H.G.: Transgenic radish (Raphanus sativus L. ssp. longipinnatus Bailey) by floral dip method — plant development and surfactant are important in optimizing transformation efficiency.-Transgenic Res. 10: 363–371, 2001.
Desfeux, C., Clough, S.J., Bent, A.F.: Female reproductive tissues are the primary target of Agrobacterium-mediated transformation by the Arabidopsis floral-dip method.-Plant Physiol. 123: 895–904, 2000.
Fang, Z.D.: Method for Study of Plant Disease. 3rd Ed.-China Agricultural Press, Beijing 1998.
Feldmann, K.A., Marks, M.D.: Agrobacterium-mediated transformation of germinating seeds of Arabidopsis thaliana: a non-tissue culture approach.-Mol. gen. Genet. 208: 1–9, 1987.
Guo, X., Huang, C., Jin, S., Liang, S., Nie, Y., Zhang, X.: Agrobacterium-mediated transformation of Cry1C, Cry2A and Cry9C genes into Gossypium hirsutum and plant regeneration.-Biol. Plant. 51: 242–248, 2007.
Katavic, V., Haughn, G.W., Reed, D., Martin, M., Kunst, L.: In planta transformation of Arabidopsis thaliana.-Mol. gen. Genet. 245: 363–370, 1994.
Koncz, C., Schell, J.: The promoter of the TL-DNA gene 5 controls the tissue-specific expression of chimeric genes carried by a novel type of Agrobacterium binary vector.-Mol. gen. Genet. 204: 383–396, 1986.
Krysan, P.J., Young, J.C., Sussman, M.R.: T-DNA as an insertional mutagen in Arabidopsis.-Plant Cell 11: 2283–2290, 1999.
Liu, F., Cao, M.Q., Yao, L., Robaglia, C., Tourneur, C.: In planta transformation of pakchoi (Brassica campestris L. ssp. chinensis) by infiltration of adult plants with Agrobacterium.-Acta Hort. 467: 187–192, 1998.
Nandakumar, R., Babu, S., Kalpana, K., Raguchander, T., Balasubramanian, P., Samiyappan, R.: Agrobacterium-mediated transformation of indica rice with chitinase gene for enhanced sheath blight resistance.-Biol Plant. 51: 142–148, 2007.
Narasimhulu, S.B., Chopra, V.L.: Species specific shoot regeneration response of cotyledonary explants of Brassicas.-Plant Cell Rep. 7: 104–106, 1988.
Richardson, K., Fowler, S., Pullen, C., Skelton, C., Morris, B., Putterill, J.: T-DNA tagging of a flowering-time gene and improved gene transfer by in planta transformation of Arabidopsis.-Aust. J. Plant Physiol. 25: 125–130, 1998.
Sambrook, J., Fritsch, E.F., Maniatis, T.: Molecular Cloning: a Laboratory Manual. 2nd Ed.-Cold Spring Harbor Laboratory Press, New York 1989.
Trieu, A.T., Burleigh, S.H., Kardailsky, L.V., Maldonado-Mendoza, L.E., Versaw, W.K., Blaylock, L.A., Shin, H., Chiou, T.J., Dewbre, G.R., Weigel, D., Harrison, M.J.: Transformation of Medicago Truncatula via infiltration of seedlings or flowering plants with Agrobacterium.-Plant J. 22: 531–541, 2000.
Yang, L., Xu, C.J., Hu, G.B.: Establishment of an Agrobacterium-mediated transformation system for Fortunella crassifolia.-Biol. Plant. 51: 541–545, 2007.
Ye, G.N., Stone, D., Pang, S.Z., Creely, W., Gonzalez, K., Hinchee, M.: Arabidopsis ovule is the target for Agrobacterium in planta vacuum infiltration transformation.-Plant J. 19: 249–257, 1999.
Zhang, F.L., Takahata, Y., Watanabe, M.: Agrobacterium-mediated transformation of cotyledonary explants of Chinese cabbage (Brassica campestris L. ssp. pekinensis).-Plant Cell Rep. 19: 569–575, 2000.
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Xu, HJ., Zhao, H., Wang, XF. et al. Exploration on the vacuum infiltration transformation of pakchoi. Biol Plant 52, 763–766 (2008). https://doi.org/10.1007/s10535-008-0148-7
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DOI: https://doi.org/10.1007/s10535-008-0148-7